Effects of Proline and Seaweed on Growth Parametres and of Green Onion Cultivation under Greenhouse Conditions
Year 2025,
Volume: 9 Issue: 3, 663 - 669, 27.09.2025
Haydar Balcı
,
Murat Kara
,
Muhsin Yıldız
Abstract
This study was conducted to investigate the effects of proline (PR), an amino acid, and seaweed (SW), which has biostimulant properties, on the growth and development of green onion (Allium cepa L.) plants. The experiment was conducted in an unheated glass greenhouse at Van Yüzüncü Yıl University using a randomized block experimental design. Key growth parameters including plant height, root length, stem diameter, number of leaves, total fresh and dry weight, and SPAD value were assessed. The results revealed that the highest plant height (29.24 cm) was recorded in the PR2+ SW1 (100 ppm proline + 3 g/L seaweed) treatment, demonstrating a significant synergistic effect compared to individual applications (p < 0.001). The longest root length was observed in the PR1+ SW2 (50 ppm proline + 5 g/L seaweed) treatment (17.02 cm), suggesting enhanced root development. The highest stem diameter (5.40 mm) was measured in the SW2 (5 g/L seaweed) treatment. Regarding biomass accumulation, the PR1+ SW2 treatment resulted in the highest total fresh weight (172.67 g), while the control group had the lowest (144.00 g). The highest dry weight (22.91 g) was recorded in the PR2+ SW1 treatment. For chlorophyll content, the PR2+ SW1 treatment yielded the highest SPAD value (69.53), though the differences were not statistically significant (p = 0.1467). These findings suggest that proline and seaweed applications can enhance growth and yield in green onion production, with the PR2+ SW1 combination providing the most favorable results. However, further studies are needed to determine the optimal application rates, assess their effectiveness under different environmental conditions, and evaluate their long-term impact on agricultural production.
Thanks
This study, entitled “Effects of Proline and Seaweed Application on Yield and Plant Growth in Green Onion Cultivation,” was presented as an oral presentation at the 5th International Symposium of Scientific Research and Innovative Studies (ISSRIS’25) held on March 21, 2025, at Bandırma Onyedi Eylül University, Balıkkesir,Turkey.
References
-
Abdelkader, M., Voronina, L., Puchkov, M., Shcherbakova, N., Pakina, E., Zargar, M. & Lyashko, M. (2023). Seed priming with exogenous amino acids improves germination rates and enhances photosynthetic pigments of onion seedlings (Allium cepa L.). Horticulturae, 9(1), 80. https://doi.org/10.3390/horticulturae9010080
-
Ananieva, E. A., Christov, K. N. & Popova, L. P. (2004). Exogenous treatment with salicylic acid leads to increased antioxidant capacity in leaves of barley plants exposed to paraquat. Physiologia Plantarum, 122(2), 221–228. https://doi.org/10.1111/j.1399-3054.2004.00405.x
-
Ashraf, M. & Foolad, M. R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59(2), 206–216. https://doi.org/10.1016/j.envexpbot.2005.12.006
-
Biancucci, M., Mattioli, R., Moubayidin, L., Sabatini, S., Costantino, P. & Trovato, M. (2015). Proline affects the size of the root meristematic zone in Arabidopsis. BMC Plant Biology, 15, 1–14. https://doi.org/10.1186/s12870-015-0641-1
-
Björkman, O. & Demmig, B. (1987). Photon yield of O₂ evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. Planta, 170(4), 489–504. https://doi.org/10.1007/BF00402983
-
Brewster, J. L. (2008). Onions and Other Vegetable Alliums. 2nd Edition. CABI Publishing. ISBN: 978-1-84593-399-9. https://doi.org/10.1079/9781845933999.0000
-
Butt, M., Ayyub, C. M., Amjad, M. & Ahmad, R. (2016). Proline application enhances growth of chili by improving physiological and biochemical attributes under salt stress. Pakistan Journal of Agricultural Sciences, 53(1), 43-49. https://doi.org/10.21162/PAKJAS/16.4539
-
Craigie, J. S. (2011). Seaweed extract stimuli in plant science and agriculture. Journal of Applied Phycology, 23(3), 371–393. https://doi.org/10.1007/s10811-010-9560-4
-
Çavuşoğlu, D. & Çavuşoğlu, K. (2020). Functions of exogenously applied proline against the negative effects of salt stress in onion (Allium cepa L.). International Journal of Environment, Agriculture and Biotechnology (IJEAB), 5(1), 197-202. https://doi.org/10.22161/ijeab/5.1.25
-
Dancey, C. P. & Reidy, J. (2004). Statistics without maths for psychology (4th ed.). Pearson Education.
-
Elsaied, M. S., Doklega, S. M., Rakha, M. & Elaidy, F. (2024). Enhancing head lettuce growth and quality under water stress with proline, melatonin, and vermicompost applications. Egyptian Journal of Soil Science, 64(3), 1207–1217. https://doi.org/10.21608/ejss.2024.123456
-
FAO. (2023). Food and Agriculture Organization statistical database. Retrieved from http://www.fao.org
-
Faraz, A., Faizan, M., Ahmad, A., Hayat, S. & Tahir, I. (2017). Foliar spray of proline enhanced the photosynthetic efficiency and antioxidant system in Brassica juncea. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(1), 112–119. https://doi.org/10.15835/nbha45110695
-
Frimpong, F., Anokye, M., Windt, C. W., Naz, A. A., Frei, M., Dusschoten, D. V. & Fiorani, F. (2021). Proline-mediated drought tolerance in barley (Hordeum vulgare L.) is associated with lateral root growth at the early seedling stage. Plants, 10(10), 2177. https://doi.org/10.3390/plants10102177
-
García, S. M. L., De Luna Vega, A., Zúñiga, C. C., Bañuelos Gutiérrez, O. A. & Silva Echeverría, M. (2014). Efecto de algas marinas en el desarrollo de Gerbera jamesonii (Asteraceae). e-CUCBA Universidad de Guadalajara, 2, 39–45.
-
Ghafoor, R., Akram, N. A., Rashid, M., Ashraf, M., Iqbal, M. & Lixin, Z. (2019). Exogenously applied proline induced changes in key anatomical features and physio-biochemical attributes in water-stressed oat (Avena sativa L.) plants. Physiology and Molecular Biology of Plants, 25, 1121–1135. https://doi.org/10.1007/s12298-019-00698-0
-
Griffiths, G., Trueman, L., Crowther, T., Thomas, B. & Smith, B. (2002). Onions: A global benefit to health. Phytotherapy Research, 16(7), 603–615. https://doi.org/10.1002/ptr.1222
-
Hernández-Herrera, R. M., Santacruz-Ruvalcaba, F., Zañudo-Hernández, J., Hernández-Carmona, G. & Vázquez-Medrano, J. (2014). Effect of liquid seaweed extracts on growth of tomato seedlings (Solanum lycopersicum L.). Journal of Applied Phycology, 26(1), 619–628.
-
Hareem, M., Danish, S., Pervez, M., Irshad, U., Fahad, S., Dawar, K. ... & Datta, R. (2024). Optimizing chili production in drought stress: Combining Zn-quantum dot biochar and proline for improved growth and yield. Scientific Reports, 14(1), 6627.
-
Hayat, S., Hayat, Q., Alyemeni, M. N., Wani, A. S., Pichtel, J. & Ahmad, A. (2012). Role of proline under changing environments: A review. Plant Signaling & Behavior, 7(11), 1456–1466. https://doi.org/10.4161/psb.21949
-
Hussain, H. I., Kasinadhuni, N. & Arioli, T. (2021). The effect of seaweed extract on tomato plant growth, productivity, and soil. Journal of Applied Phycology, 33(2), 1305–1314. https://doi.org/10.1007/s10811-021-02387-2
-
Irshad, I., Anwar-Ul-Haq, M., Akhtar, J. & Maqsood, M. (2024). Enhancing maize growth and mitigating salinity stress through foliar application of proline and glycine betaine. Pakistan Journal of Botany, 56(1), 9–17.
-
Kavi Kishor, P. B., Hima Kumari, P., Sunita, M. S. L. & Sreenivasulu, N. (2015). Role of proline in cell wall synthesis and plant development and its implications in plant ontogeny. Frontiers in Plant Science, 6, 544. https://doi.org/10.3389/fpls.2015.00544
-
Khan, Z. H., Qadir, I., Yaqoob, S., Khan, R. A. & Khan, M. A. (2009). Response of range grasses to salinity levels at germination and seedling stage. J. Agric. Res. (Lahore), 47(2), 179–184.
-
Koç, A., Yılmaz, B. & Demir, C. (2024). Prolin uygulamasının pirinç (Oryza sativa L.)'de bitki boyu ve fizyolojik parametreler üzerine etkileri. Tarım ve Doğa Bilimleri Dergisi, 12(3), 45–56 (in Turkish).
-
Lynch, J. P. (2013). Steep, cheap, and deep: An ideotype to optimize water and N acquisition by maize root systems. Annals of Botany, 112(2), 347–357. https://doi.org/10.1093/aob/mcs293
-
Mukaka, M. M. (2012). A guide to appropriate use of Correlation coefficient in medical research. Malawi Medical Journal, 24(3), 69–71. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576830/
-
Nabti, E., Jha, B. & Hartmann, A. (2021). Impact of seaweed biofertilizers on tomato (Solanum lycopersicum) growth and soil microbial activity. Plants, 10(3), 621. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000310/
-
Pervaiz, A., Iqbal, A., Khalid, A., Manzoor, A., Noreen, S., Ayaz, A., Zafar, Z. U., Athar, H.-u.-R. & Ashraf, M. (2019). Proline induced modulation in physiological responses in wheat plants. Journal of Agriculture and Environmental Sciences, 8(1), 112–119.
-
Poorter, H., Niklas, K. J., Reich, P. B., Oleksyn, J., Poot, P. & Mommer, L. (2012). Biomass allocation to leaves, stems, and roots: Meta‐analyses of interspecific variation and environmental control. New Phytologist, 193(1), 30–50. https://doi.org/10.1111/j.1469-8137.2011.03952.x
-
Santos, J. M., Oliveira, L. F. & Pereira, R. S. (2024). Effects of Proline Applications on Plant Growth and Enzyme Activities in Forage Pea (Pisum sativum ssp. arvense L.) under Different Water Limit Conditions. Journal of Plant Physiology, 180(3), 245-253.
-
Semida, W. M., Abdelkhalik, A., Rady, M. M. & Abd El-Mageed, T. A. (2020). Exogenously applied proline enhances growth and productivity of drought-stressed onion by improving photosynthetic efficiency, water use efficiency, and up-regulating osmoprotectants. Scientia Horticulturae, 272, 109580. https://doi.org/10.1016/j.scienta.2020.109580
-
Shukla, P. S., Shotton, K., Norman, E., Neily, W., Critchley, A. T. & Prithiviraj, B. (2021). Seaweed extract improves sugarcane growth and yield by modulating plant hormone metabolism. Frontiers in Plant Science, 12, 659130. https://www.frontiersin.org/articles/10.3389/fpls.2021.659130/full
-
Soleimani, M., Rahimi, A. & Bahrami, H. (2024). Potential of Liquid Extracts of Sargassum wightii on Growth, Biochemical, and Yield Parameters of Cluster Bean Plant. Journal of Applied Phycology, 36(2), 1023-1035.
-
Szabados, L. & Savouré, A. (2010). Proline: a multifunctional amino acid. Trends in Plant Science, 15(2), 89-97. https://doi.org/10.1016/j.tplants.2009.11.009
-
Turkish Statistical Institute (TurkStat). (2023). Agricultural data of Turkey. Retrieved from https://www.tuik.gov.tr
-
Xu, X., Gianoli, E. & Zhang, L. (2018). Ecophysiological correlates of leaf traits in ferns: A comparative study across species and habitats. Frontiers in Ecology and Evolution, 6, 64. https://doi.org/10.3389/fevo.2018.00064
-
Yamaguchi-Shinozaki, K. & Shinozaki, K. (2006). Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annual Review of Plant Biology, 57, 781–803. https://doi.org/10.1146/annurev.arplant.57.032905.105444