The use of Marrubium vulgare L. plant extracts in the control of fungal plant pathogens

Year 2020, , 476 - 482, 15.12.2020

Oğuz Özbek , Yakup Budak , Çağlar Berkel , Çiğdem Özyiğit , Yusuf Yanar

https://doi.org/10.31015/jaefs.2020.4.11

Cited By: 10

Abstract

In this study, we investigated anti-fungal properties of Marrubium vulgare L. plant extracts dissolved in four different solvents against five different plant pathogenic fungi Fusarium oxysporum f. sp. lycopersici, Macrophamina phaseolina, Phytopthora infestans, Sclerotinia sclerotiorum and Rhizoctania solani. We used 1.0%, 2.5% and 5.0% plant extract concentrations against these fungi species which cause significant decrease in production yield of certain plants. In all pathogenic fungi species analyzed, we observed an increase in the anti-fungal effect of Marrubium vulgare L. plant extract preparations in a dose-dependent manner. Marrubium vulgare L. extracts dissolved in chloroform and dichloromethane at 2.5% concentration showed higher inhibition against Fusarium oxysporum compared to other solvents at this concentration. At the same concentration, extracts dissolved in methanol resulted in higher anti-fungal activity against Phytopthora infestans and intermediate activity against Macrophamina phaseolina and Sclerotinia sclerotiorum. As a result, it can be stated that Marrubium vulgare L. plant extracts dissolved in different solvents display anti-fungal properties against certain plant pathogens.

Keywords

Marrubium Vulgare L, anti-fungal activity, plant extracts, pathogen

Supporting Institution

Scientific Research Projects Commission of Tokat Gaziosmanpasa University

Project Number

2018/62

References

• Amri, B., Martino, E., Vitulo, F., Corana, F., Kaab, L.B., Rui, M., Rossi, D., Mori, M., Rossi, S. and Collina, S. (2017). Marrubium vulgare L. leave extract: phytochemical composition, antioxidant and wound healing properties. Molecules, 22, 1851-1869. https://doi.org/10.3390/molecules22111851
• Argyropoulou, A., Samara, P., Tsitsilonis, O. and Skaltsa, H. (2012) Polar constituents of Marrubium thessalum Boiss. & Heldr. (Lamiaceae) and their cytotoxic/cytostatic activity. Phytotheraphy Research, 26, 1800-1806. https://doi.org/10.1002/ptr.4654
• Bardai, S.E., Lyoussi, B., Wibo, M. and Morel, N. (2001). Pharmacological evidence of hypotensive activity of Marrubium vulgare and Foeniculum vulgare in spontaneously hypertensive rat. Clinical and Experimental Hypertension, 23, 329-343. https://doi.org/10.1081/CEH-100102671 Benner, J.P. (1996). Pesticides from nature. Part I. Crop protection agents from higher plants-an overview. In: Copping LG (Ed). The Royal Society of Chemistry, London, pp. 217-229.
• Bokaeian, M., Saboori, E., Saeidi, S., Niazi, A.A., Amini-Borojeni, N., Khaje, H. and Bazi, S. (2014). Phytochemical analysis, antibacterial activity of Marrubium vulgare L. against Staphylococcus aureus in vitro. Zahedan Journal of Research in Medical Sciences, 16, 60-64. https://www.sid.ir/en/journal/ViewPaper.aspx?id=470153
• Boudjelal, A., Henchiri, C., Siracusa, L., Sari, M. and Ruberto, G. (2012). Compositional analysis and in vivo anti-diabetic activity of wild Algerian Marrubium vulgare L. infusion. Fitoterapia, 83, 286-292. https://doi.org/10.1016/j.fitote.2011.11.005
• Bouterfas, K., Mehdadi, Z., Maliha Elaoufi, M., Aouad, L., Latreche, A., and Benchiha, W. (2018). In vitro antibacterial activity of flavonoids extracts from three Algerian horehound (Marrubium vulgare L.) leaves. Oriental Pharmacy and Experimental Medicine, 18, 59-66. https://doi.org/10.1007/s13596-017-0287-5
• Copping, L.G. and Menn, J.J. (2000). Biopesticides: a review of their action, applications and efficacy. Pest Management Science, 56, 651-676. https://doi.org/10.1002/1526-4998(200008)56:8<651::AID-PS201>3.0.CO;2-U
• Deans, S.G. and Sobada, K.P. (1990). Antimicrobial properties of Marjoram (Origanum marjorana L.) volatile oil. Flavour and Fragrance Journal, 5, 187-190. https://doi.org/10.1002/ffj.2730050311
• Gautam, K., Rao, P.B. and Chauhan, S.V.S. (2003). Efficacy of some botanicals of the family compositae against Rhizoctonia solani Kuhn. Indian Journal of Mycology and Plant Pathology 33, 230-235. https://agris.fao.org/agris-search/search.do?recordID=IN2004000123
• Gonza´lez, M.J. and Marioli, J.M. (2010). Antibacterial activity of water extracts and essential oils of various aromatic plants against Paenibacillus larvae, the causative agent of American Foulbrood. Journal of Invertebrate Pathology, 104, 209-213. https://doi.org/10.1016/j.jip.2010.04.005
• Gruenwald, J., Brendler, T., Jaenicke, C., Fleming, T., Deutsch, M. and Hamid, M. (1998). PDR for Herbal Medicines.
• Gürdere, M. B., Aydın, A., Yencilek, B., Ertürk, F., Özbek, O., Erkan, S., Budak, Y. and Ceylan, M. (2020). Synthesis, antiproliferative and cytotoxic activities, DNA binding features and molecular docking study of novel enamine derivatives. Chemistry & Biodiversity, 17(7), e2000139. https://doi.org/10.1002/cbdv.202000139
• Kassambara, A. (2020). ggpubr: 'ggplot2' Based publication ready plots. R package version 0.4.0. https://CRAN.R-project.org/package=ggpubr
• Montvale Koul, P. and Dhaliwal, G.S. (2001). Microbial Biopesticides. New York
• Marcin, N., Majid, F. and Nowakow, M.R. (2012). Potato and tomato late blight caused by Phytophthora infestans: An overview of pathology and resistance breeding. Plant Disease, 96, 4-17 .https://doi.org/10.1094/PDIS-05-11-0458
• Meyre-Silva, C., Yunes, R., Schlemper, V., Campos-Buzzi, F. and Cechinel Filho, V. (2005). Analgesic potential of marrubiin derivatives, a bioactive diterpene present in Marrubium vulgare (Lamiaceae). II Farmaco 60, 321-326. https://doi.org/10.1016/j.farmac.2005.01.003
• Özbek, O., Usta, N.C., Gürdere, M.B., Aslan, O.N., Budak, Y. and Ceylan, M. (2017). Synthesis and antibacterial screening of novel 2-(4-(aryl) thiazol-2-yl)-3a,4,7,7a-tetrahydro-1H-4,7-ethanoisoindole-1,3(2H)-dione derivatives. Phosphorus, Sulfur, and Silicon and the Related Elements, 192(10), 1153–1157. https://doi.org/10.1080/10426507.2017.1354209
• Özbek, O. and Gürdere, M.B. (2020). A review on the synthesis and applications of molecules as anticonvulsant drug agent candidates. Medicinal Chemistry Research, 29, 1153-1178. https://doi.org/10.1007/s00044-020-02595-4
• R Core Team (2018). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/
• Rai, G.K., Kumar, R., Singh, J., Rai, P.K. and Rai, S.K. (2011). Peroxidase, Polyphenol oxidase activity. protein profile and phenolic content in tomato cultivars tolerant susceptible to Fusarium oxysporum f.sp.lycopersici. Pakistan Journal of Botany, 43, 2987-2990.
• Roman Ramos, R., Alarcon-Aguilar, F., Lara-Lemus, A. and Flores-Saenz, J.L. (1992). Hypoglycemic effect of plants used in Mexico as antidiabetics. Archives of Medical Research, 23, 59-64.
• Schlemper, V., Ribas, A., Nicolau, M. and Cechinel-Filho, V. (1996). Antispasmodic effects of hydroalcoolic extract of Marrubium vulgare on isolated tissues. Phytomedicine, 3, 211-216.https://doi.org/10.1016/S0944-7113(96)80038-9
• Shuping, D. and Eloff, J.N. (2017). The use of plants to protect plants and food against fungal pathogens: a review. African Journal of Traditional, Complementary, and Alternative Medicines, 14(4), 120-127. https://doi.org/10.21010/ajtcam.v14i4.14
• Yamaguchi, K., Liggett, J.L., Kim, N-C. and Beak, S.J. (2006). Anti-proliferative effect of horehound leaf and wild cherry bark extracts on human colorectal cancer cells. Oncology Reports, 15, 275-282. https://doi.org/10.3892/or.15.1.275
• Visser, D.D. (2007). Studies on Sclerotinia sclerotiorum (Sclerotinia stem rot) on soybeans (MSc Thesis), University of KwaZulu-Natal, South Africa.
• Weel, K.G.C., Venskutonis, P.R., Pukalskas, A., Gruzdiene, D. and Linssen, J.P.H. (1999). Antioxidant activity of horehound (Marrubium vulgare L.) grown in Lithuani. European Journal of Lipid Science and Technology 101, 395-400. https://doi.org/10.1002/(SICI)1521-4133(199910)101:10<395::AID-LIPI395>3.0.CO;2-L
• Wickham, H., Averick, M., Bryan, J., Chang, W., McGowan, L.D., François, R., Garrett, G., Hayes, A., Henry, L., Hester, J., Kuhn, M., Pedersen, T.L., Miller, E., Bache, S.M., Müller, K., Ooms, J., Robinson, D., Seidel, D.P., Spinu, V., Takahashi, K., Vaughan, D., Wilke, C., Woo, K. and Yutani, H. (2019). Welcome to the tidyverse. Journal of Open Source Software, 4, 1686-1692. https://doi.org/10.21105/joss.01686
• Wickham, H. and Bryan, J. (2019). readxl: Read Excel Files. R package version 1.3.1. https://CRAN.R-project.org/package=readxl