The use of Marrubium vulgare L. plant extracts in the control of fungal plant pathogens
Abstract
In this study, we investigated anti-fungal properties of Marrubium vulgare L. plant extracts dissolved in four different solvents against five different plant pathogenic fungi Fusarium oxysporum f. sp. lycopersici, Macrophamina phaseolina, Phytopthora infestans, Sclerotinia sclerotiorum and Rhizoctania solani. We used 1.0%, 2.5% and 5.0% plant extract concentrations against these fungi species which cause significant decrease in production yield of certain plants. In all pathogenic fungi species analyzed, we observed an increase in the anti-fungal effect of Marrubium vulgare L. plant extract preparations in a dose-dependent manner. Marrubium vulgare L. extracts dissolved in chloroform and dichloromethane at 2.5% concentration showed higher inhibition against Fusarium oxysporum compared to other solvents at this concentration. At the same concentration, extracts dissolved in methanol resulted in higher anti-fungal activity against Phytopthora infestans and intermediate activity against Macrophamina phaseolina and Sclerotinia sclerotiorum. As a result, it can be stated that Marrubium vulgare L. plant extracts dissolved in different solvents display anti-fungal properties against certain plant pathogens.
Keywords
Marrubium Vulgare L, anti-fungal activity, plant extracts, pathogen
Supporting Institution
Scientific Research Projects Commission of Tokat Gaziosmanpasa University
Project Number
2018/62
References
- Amri, B., Martino, E., Vitulo, F., Corana, F., Kaab, L.B., Rui, M., Rossi, D., Mori, M., Rossi, S. and Collina, S. (2017). Marrubium vulgare L. leave extract: phytochemical composition, antioxidant and wound healing properties. Molecules, 22, 1851-1869. https://doi.org/10.3390/molecules22111851
- Argyropoulou, A., Samara, P., Tsitsilonis, O. and Skaltsa, H. (2012) Polar constituents of Marrubium thessalum Boiss. & Heldr. (Lamiaceae) and their cytotoxic/cytostatic activity. Phytotheraphy Research, 26, 1800-1806. https://doi.org/10.1002/ptr.4654
- Bardai, S.E., Lyoussi, B., Wibo, M. and Morel, N. (2001). Pharmacological evidence of hypotensive activity of Marrubium vulgare and Foeniculum vulgare in spontaneously hypertensive rat. Clinical and Experimental Hypertension, 23, 329-343. https://doi.org/10.1081/CEH-100102671 Benner, J.P. (1996). Pesticides from nature. Part I. Crop protection agents from higher plants-an overview. In: Copping LG (Ed). The Royal Society of Chemistry, London, pp. 217-229.
- Bokaeian, M., Saboori, E., Saeidi, S., Niazi, A.A., Amini-Borojeni, N., Khaje, H. and Bazi, S. (2014). Phytochemical analysis, antibacterial activity of Marrubium vulgare L. against Staphylococcus aureus in vitro. Zahedan Journal of Research in Medical Sciences, 16, 60-64. https://www.sid.ir/en/journal/ViewPaper.aspx?id=470153
- Boudjelal, A., Henchiri, C., Siracusa, L., Sari, M. and Ruberto, G. (2012). Compositional analysis and in vivo anti-diabetic activity of wild Algerian Marrubium vulgare L. infusion. Fitoterapia, 83, 286-292. https://doi.org/10.1016/j.fitote.2011.11.005
- Bouterfas, K., Mehdadi, Z., Maliha Elaoufi, M., Aouad, L., Latreche, A., and Benchiha, W. (2018). In vitro antibacterial activity of flavonoids extracts from three Algerian horehound (Marrubium vulgare L.) leaves. Oriental Pharmacy and Experimental Medicine, 18, 59-66. https://doi.org/10.1007/s13596-017-0287-5
- Copping, L.G. and Menn, J.J. (2000). Biopesticides: a review of their action, applications and efficacy. Pest Management Science, 56, 651-676. https://doi.org/10.1002/1526-4998(200008)56:8<651::AID-PS201>3.0.CO;2-U
- Deans, S.G. and Sobada, K.P. (1990). Antimicrobial properties of Marjoram (Origanum marjorana L.) volatile oil. Flavour and Fragrance Journal, 5, 187-190. https://doi.org/10.1002/ffj.2730050311
- Gautam, K., Rao, P.B. and Chauhan, S.V.S. (2003). Efficacy of some botanicals of the family compositae against Rhizoctonia solani Kuhn. Indian Journal of Mycology and Plant Pathology 33, 230-235. https://agris.fao.org/agris-search/search.do?recordID=IN2004000123
- Gonza´lez, M.J. and Marioli, J.M. (2010). Antibacterial activity of water extracts and essential oils of various aromatic plants against Paenibacillus larvae, the causative agent of American Foulbrood. Journal of Invertebrate Pathology, 104, 209-213. https://doi.org/10.1016/j.jip.2010.04.005
