Year 2020, Volume 4 , Issue 4, Pages 394 - 399 2020-12-15

The antioxidant and antialzhemier activities of the Diplotaenia turcica with phytochemical analysis

Ugur OZDEK [1]


Diplotaenia turcica is an endemic plant that grows in eastern Turkey. This herb is used in herbal cheese, in meals and in traditional therapies. In this study, we aimed to examine some of the biochemical activities of this plant. Liquid chromatography-mass spectrometry (LC-MS) analysis was conducted on hydro alcohol extract of aerial part of DT. This analysis was applied to determine the total phenolic and flavonoid content, antioxidant and anti-alzheimer activities. LC-MS analysis showed that malic acid and quinic acid were found to be major compounds. The key flavonoids detected were hesperidine and rutine. The end of examination, the total amount of phenolic compound of extract was measured as 27.54 μg PEs/mg. And the total flavonoid amount was measured as 7.31 μg KEs/mg. β-carotene-linoleic acid test, DPPH free radical scavenging method, and ABTS cation radical scavenging outcomes were determined as IC50 of 169.71 μg/mL, 164.42 and 68.74 μg/mL, respectively. Cholinesterase inhibition test which indicate of alzheimer's did not inhibit acetylcholinesterase (AChE), but butyrylcholinesterase (BCHE) enzyme inhibition was 76.57%. As a result, further studies are needed in order to use Diplotaenia turcica plant for treatment or support purposes in the health field.
Diplotaenia turcica, Antialzhemier, Antioxidant, Activities
  • Apak, R., Güçlü, K., Özyürek, M., and Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of agricultural and food chemistry, 52(26), 7970-7981. Doi: https://doi.org/10.1021/jf048741x
  • Boğa, M., Hacıbekiroğlu, I., and Kolak, U. (2011). Antioxidant and anticholinesterase activities of eleven edible plants. Pharmaceutical biology, 49(3), 290-295. Doi: https://www.tandfonline.com/doi/full/10.3109/13880209.2010.517539
  • Çölçimen, N., Özdek, U., Değer, Y., Altındağ, F., and Arihan, O. (2019). Investigation of the effects of diplotaenia turcica plant root extract on rat over tissue by histological and stereological methods. Fresenius Environmental Bulletin, 28(4), 2407-2412. Retrieved from https://www.prt-parlar.de/download_feb_2019/
  • Ertaş, A., Boğa, M., Yılmaz, M. A., Yeşil, Y., Haşimi, N., Kaya, M. S. e., . . . Kolak, U. (2014). Chemical compositions by using LC-MS/MS and GC-MS and biological activities of Sedum sediforme (Jacq.) Pau. Journal of agricultural and food chemistry, 62(20), 4601-4609. Doi: https://pubs.acs.org/doi/abs/10.1021/jf500067q
  • Frankel, E. N., Huang, S.-W., Kanner, J., and German, J. B. (1994). Interfacial phenomena in the evaluation of antioxidants: bulk oils vs emulsions. Journal of agricultural and food chemistry, 42(5), 1054-1059. Doi: https://pubs.acs.org/doi/10.1021/jf00041a001
  • Kähkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J.-P., Pihlaja, K., Kujala, T. S., and Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of agricultural and food chemistry, 47(10), 3954-3962. Doi: https://pubs.acs.org/doi/10.1021/jf990146l
  • Kaval, I., Behçet, L., and Cakilcioglu, U. (2014). Ethnobotanical study on medicinal plants in Geçitli and its surrounding (Hakkari-Turkey). Journal of Ethnopharmacology, 155(1), 171-184. Doi: https://doi.org/10.1016/j.jep.2014.05.014
  • Koleva, I. I., Van Beek, T. A., Linssen, J. P., Groot, A. d., and Evstatieva, L. N. (2002). Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 13(1), 8-17. Retrieved from https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.611
  • Kosanić, M., Ranković, B., and Dašić, M. (2012). Mushrooms as possible antioxidant and antimicrobial agents. Iranian journal of pharmaceutical research: IJPR, 11(4), 1095. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813146/
  • Meydan, İ., Kizil, G., Demir, H., Toptanci, B. C., and Kizil, M. (2020). In vitro DNA damage, protein oxidation protective activity and antioxidant potentials of almond fruit (Amygdalus trichamygdalus) parts (hull and drupe) using soxhlet ethanol extraction. Advances in Traditional Medicine, 1-9. Doi: https://doi.org/10.1007/s13596-020-00491-1
  • Moreno, M. N., Isla, M., Sampietro, A. R., and Vattuone, M. A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71(1), 109-114. Retrieved from https://pubmed.ncbi.nlm.nih.gov/10904153/
  • Nordberg, J., and Arner, E. S. (2001). Reactive oxygen species, antioxidants, and the mammalian thioredoxin system1. Free Radical Biology and Medicine, 31(11), 1287-1312. Retrieved from https://www.sciencedirect.com/science/article/pii/S0891584901007249
  • Özdek, U., Başbuğan, Y., Yıldırım, S., Fırat, M., and Değer, Y. (2018). Activity, acute and sub-acute toxicity and safety assesment of the hydroalcholic root extract of Diplotaenia turcica. Indian Journal of Animal Research, 52(12), 1688-1694. Retrieved from https://arccjournals.com/uploads/Final-attachment-published-B-901.pdf
  • Özdek, U., Seçkin, H., and çibuk, S. (2020). Investigation of Antimicrobial Effects of Amygdalus Trichamygdalus (Sweet Almond) and Amygdalus nana L.(Bitter Almond) Plants. Van Veterinary Journal, 31(1), 22-26. Doi: https://doi.org/10.36483/vanvetj.651515
  • Özdek, U., Yıldırım, S., and Değer, Y. (2020). The effect of Diplotaenia turcica root extract in streptozotocin-induced diabetic rats. Turkish Journal of Biochemistry, 45(2), 1-10. Retrieved from https://www.degruyter.com/view/journals/tjb/45/2/article-20180411.xml
  • Papetti, A., Daglia, M., Grisoli, P., Dacarro, C., Gregotti, C., and Gazzani, G. (2006). Anti-and pro-oxidant activity of Cichorium genus vegetables and effect of thermal treatment in biological systems. Food Chemistry, 97(1), 157-165. Doi: https://doi.org/10.1016/j.foodchem.2005.03.036
  • Pattanayak, P., Behera, P., Das, D., and Panda, S. K. (2010). Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacognosy reviews, 4(7), 95. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249909/
  • Raskind, M. A., Peskind, E. R., Truyen, L., Kershaw, P., and Damaraju, C. V. (2004). The cognitive benefits of galantamine are sustained for at least 36 months: a long-term extension trial. Arch Neurol, 61(2), 252-256. Retrieved from https://jamanetwork.com/journals/jamaneurology/article-abstract/785390
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. Doi: https://doi.org/10.1016/S0891-5849(98)00315-3
  • Rice-Evans, C., Miller, N., and Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends in plant science, 2(4), 152-159. Doi: https://doi.org/10.1016/S1360-1385(97)01018-2
  • Scandalios, J. G. (2002). The rise of ROS. Trends in biochemical sciences, 27(9), 483-486. Retrieved from https://www.cell.com/action/showPdf?pii=S0968-0004%2802%2902170-9
  • Simonian, N., and Coyle, J. (1996). Oxidative stress in neurodegenerative diseases. Annual review of pharmacology and toxicology, 36(1), 83-106. Retrieved from https://www.annualreviews.org/doi/abs/10.1146/annurev.pa.36.040196.000503?journalCode=pharmtox
  • Slinkard, K., and Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American journal of enology and viticulture, 28(1), 49-55. Retrieved from https://www.ajevonline.org/content/28/1/49.short
  • Uce, İ., and Tunçtürk, M. (2014). Wild Plants which Naturally Grown and Widely Used in Hakkari Research Journal of Biology Sciences, 7(2), 21-25. Retrieved from http://bibad.gen.tr/index.php/bibad/article/view/244
  • Winrow, V., Winyard, P., Morris, C., and Blake, D. (1993). Free radicals in inflammation: second messengers and mediators of tissue destruction. British Medical Bulletin, 49(3), 506-522. Doi: https://doi.org/10.1093/oxfordjournals.bmb.a072627
  • Yuksek, V., Cetin, S., Usta, A., Komuroglu, A. U., and Dede, S. (2017). Effect of Some Vitamins on Antioxidant/Prooxidant Parameters in Sodium Fluoride (NaF)-Treated Cell Line (hFOB 1.19). Turkish Journal of Veterinary Research, 1(1), 1-6. Retrieved from https://dergipark.org.tr/en/download/article-file/384088
Primary Language en
Subjects Environmental Sciences, Soil Science
Published Date December 2020
Journal Section Research Articles
Authors

Orcid: 0000-0002-0709-1545
Author: Ugur OZDEK (Primary Author)
Institution: Van Yuzuncu Yil University, Vocational School of Health Services, 65080 Van, Turkey
Country: Turkey


Thanks I would like to thank Mr. Mehmet Fırat for taxonomic examination of the plant and Mr. Mehmet Boğa for his support.
Dates

Application Date : July 24, 2020
Acceptance Date : September 23, 2020
Publication Date : December 15, 2020

APA Ozdek, U . (2020). The antioxidant and antialzhemier activities of the Diplotaenia turcica with phytochemical analysis . International Journal of Agriculture Environment and Food Sciences , 4 (4) , 394-399 . DOI: 10.31015/jaefs.2020.4.1