Research Article
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Year 2021, Volume 5, Issue 3, 398 - 404, 15.09.2021
https://doi.org/10.31015/jaefs.2021.3.19

Abstract

References

  • Adams RP.(1995). Identification of essential oil components by gas chromatography/masss spectrometry. Allured Publishing: Carol Stream, IL,.
  • Adwan G, Mhanna, M. (2009). Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Asian Pacific Journal of Tropical Medicine,2: 3, 46-51.
  • Ates DA, Erdogrul OT.(2003). Antimicrobial activity of various medicinal and commercial plants extracts. Turkish Journal of Biology, 27: 157-162. Retrieved from https://dergipark.org.tr/tr/pub/tbtkbiology/issue/11734/140074
  • Baser K.H.C, N. Kirimer and G. Tümen.(2014). Composition of the essential oil of Origanum majorana L. from Turkey. Journal of Essential Oil Research,5,577-579. Doi: https://doi.org/10.1080/10412905.1993.9698283
  • Baydar H, Sagdic O, Ozkan G, Karadogan T.(2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control, 15,169–72. Doi: https://doi.org/10.1016/S0956-7135(03)00028-8
  • Ben A.A., Combes S, Preziosi-Belloy L, Gontard N, Chalier P.(2006). Antimicrobial activity of carvacrol related to its chemical structure. Letters in Applied Microbiology,.Aug43(2):149-54. Doi: https://doi.org/10.1111/j.1472-765X.2006.01938.x
  • Ben, H. E. N., M. M. Abdelkefi, R. B. Aissa and M. M. Chabouni.(2001). Antibacterial screening of Origanum majorana L. oil from Tunisia. Journal of Essential Oil Research, 13, 295-297. Bnyan I.A., Aumaima
  • Tariq Abid, Hamid Naji Obied.(2014). Antibacterial Activity of Carvacrol against Different Types of. Bacteria. Journal of Natural Sciences Research, 4(9), 13-16.
  • Burt S.(2000). Essential oils: their antibacterial properties andpotential applications in foods - a review. International Journal of Food Microbiology, 94, 223-253. Doi: https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  • Busatta C, Vidal R. S, Popiolski A. S, Mossi A. J, Dariva C, Rodrigues M. R. A, Corazza F. C, Corazza M. L, Oliveira J. V & Cansian, R. L. (2008). Application of (Origanum majorana L.) essential oil as an antimicrobial agent in sausage. Food Microbiology, 25(1), 207-211. Doi: https://doi.org/10.1016/j.fm.2007.07.003
  • Daferea D. J, Ziogas, B. N. and Polissiou, M. G.(2000). GC-MS analysis of essential oils from some Greek aromatic plants and their fungitoxicity on Penicillium digitatum. Journal of Agriculture Food Chemistry, 48, 2576-2581. Doi: https://doi.org/10.1021/jf990835x
  • Deans SG, and Svoboda KP. (1990). The antimicrobial properties of Marjoram (Origanum majorana L.) volatile oil. Flavour and Fragrance Journal; 5, 187–190. Doi: https://doi.org/10.1002/ffj.2730050311
  • Dorman HJD, Deans SG.(2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology, 88,308-316. Doi: http://dx.doi.org/10.1046/j.1365-2672.2000.00969.x
  • Ezzeddine N.B, Abdelkafi M.M, Ben Aissa R & Chaabouni, M.M.(2001). Antibacterial screening of (Origanum majorana L.), oil from Tunisia. Journal of Essential Oil Research,13,295-297.
  • Farooqi A. A. and B. S. Sreeramu.(2004). Cultivation of medicinal and aromatic crops. Universities Press, India pp. 465-470. Doi: https://doi.org/10.1080/10412905.2001.9699698
  • Hammer KA, Carson CF, Riley TV. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbioogyl, 86,985–90. Doi: https://doi.org/10.1046/j.1365-2672.1999.00780.x
  • Helal G. A, Sarhan M. M, Abu Shahla A. N. K, Abou El-Khair E. K.(2006). Antimicrobial activity of some essential oils against microorganisms deteriorating fruit juices. Mycobiology, 34(4), 219-229. Doi: https://dx.doi.org/10.4489%2FMYCO.2006.34.4.219
  • Holley R.A, Patel D.(2005). Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiology, 22, 273-292. Doi: https://doi.org/10.1016/j.fm.2004.08.006
  • Janssen A. M, Scheffer, J. J. C. and Baerheim-Svendeaen A. (1987). Antimicrobial activity of essential oils: a 1976-86 literature review. Aspects of the test methods. Doi: https://doi.org/10.1007/BF02029329
  • Kim K.J, Yu, H.H., Cha J.D, Seo S.J, Choi, N.Y., You, YO.(2005) Antibacterial activity of Curcuma longa L. against methicillin-resistant Staphylococcus aureus. Phytotheraphy Research, 19(7),599-604. Doi: https://doi.org/10.1002/ptr.1660
  • Kirtikar K. R. and B. D. Basu.(1985). Indian medicinal plants. (Eds.: E. Blatter, J.F.Caius and S.K. Mhaskar). Bishen Singh and Mahendra Pal Sing,Dehradun. 1985; Vol. 3, pp. 250.
  • Lambert RJW, Skandamis PN, Coote PJ, Nychas GJE.(2001). A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology,91,453–62. Doi: https://doi.org/10.1046/j.1365-2672.2001.01428.x
  • Leeja L& Thoppil J.E.(2007). Antimicrobial activity of methanol extract of (Origanum majorana L.) (sweet marjoram). Journal of Environmental Biology, 28 (1),145-146.
  • Mishra, A. K. and Dubey, N. K. (1994). Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Applied and Environmental. Microbiology, 60, 1101-1105.
  • Mohamed N, Yasmen SH, Nohir G. (2011). Antimicrobial activity of water and ethanol Marjoram (Origanum marjorana L.) extract. The 6th Arab and 3rd International Annual Scientific Conference, 2350-2366.
  • Novak J, F. Pank, J. Langbehn, W.D. Blüthner, C. Vender, L.V.Niekerk, W. Junghanns, C. Franz.(2004). Determination of growing location of marjoram (Origanum majorana L.) samples by comparison of essential oil profiles. Flavour Fragrance Journal, 19, 263-267.
  • Ozdemir G, Karabay NU, Conk Dalay M, Pazarbası B.(2004). Antimicrobial activity of volatile component and various extracts of Spirulina plantensis. Phototherapy Research, 18,754-757. Doi: https://doi.org/10.1002/ptr.1541
  • Pernin A, Guillier L, Dubois-Brissonnet F.(2019). Inhibitory activity of phenolic acids against Listeria monocytogenes: deciphering the mechanisms of action using three different models.  Food Microbiology,  80,18–24. Doi: https://doi.org/10.1016/j.fm.2018.12.010
  • Phadke SA and Kulkarni SD.(1989). Screening of in vitro antibacterial activity of Terminalia chebula, Eclapta alba and Ocimum sanctum. Indian Journal of Medical Sciences,43(5), 113-117.
  • Pino J.A., A. Rosado, M. Estarron, V. Fuentes.(1997). Essential oil of Marjoram (Origanum majorana L.) grown in Cuba. Journal of Essential Oil Research,9, 481-482.
  • Prudent D, Perineau F, Bessiere J. M, Michel, G. M. and Baccou,J. C(1995). Analysis of the essential oil of wild oregano form Martinique (Coleus aromaticus Benth.). Evaluation of its bacteriostatic and fungistatic properties. J. Essent. Oil Res, 7,165-173. Doi: https://doi.org/10.1080/10412905.1995.9698492
  • Ramos S, Luis B. Rojas, Maria Eugenia Lucena, Gina Meccia & Alfredo Usubillaga. (2011). Chemical composition and antibacterial activity of Origanum majorana L. essential oil from the Venezuelan Andes, Journal of Essential Oil Research,23:5,45-49. Doi: https://doi.org/10.1080/10412905.2011.9700481
  • Selim S.A., Mohamed H. Abdel Aziz, Mona S. Mashait and Mona F. Warrad.(2013). Antibacterial activities, chemical constitutes and acute toxicity of Egyptian Origanum majorana L., Peganum harmala L. and Salvia officinalis L. essential oils. African Journal of Pharmcy and Pharmacology,7(13), 725-735. Doi: http://dx.doi.org/10.5897/AJPP2013.3518
  • Shu C. K. and Lawrence, B. M.(1997). Reasons for the variation in composition of some commercial essential oils. pp 138-159.In: S. J. Risch and C. T. Ho. Eds. ACS symposium series: Col.660. Spices, Flavor chemistry and antioxidant properties.
  • Vági E, B. Simándi, A. Suhajda, E. Héthelyi.(2005). Essential oil composition and antimicrobial activity of Origanum majorana L. extracts obtained with ethyl alcohol and supercritical carbon dioxide. Food Research International, 38, 51-57. Doi: http://dx.doi.org/10.1016/j.foodres.2004.07.006
  • Vardar-Ünlü G, Candan F, Sokmen A, Daferera D, PolissiouM, Sokmen M, Donmez, E. and Tepe B. (2003). Antibacterial and antioxidant activity of the essential oil and methanol extracts of Thymus pectinatus Fisch. et Mey var. Pectinatus (Lamiaceae). Journal of Agriculture and Food Chemistry, 51, 63-67. Doi: https://doi.org/10.1021/jf025753e

Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey

Year 2021, Volume 5, Issue 3, 398 - 404, 15.09.2021
https://doi.org/10.31015/jaefs.2021.3.19

Abstract

To determine the chemical composition and antimicrobial potential of marjoram (Origanum majorana L.) leaves extratcs obtained from Muğla, Turkey. The extract of ethanol, methanol, hexane, chloroform, isopropanol and water of marjoram were tested for antimicrobial activity against eleven bacterial strains and one yeast by disc diffusion method. The minimum inhibitory concentrations (MICs) of isopropanolic extract of marjoram leaves were determined using broth serial dilution method. The constitituents were analyzed by gas chromatography-mass spectrometry (GC/MS). The Shapiro-Wilk of Normal Distribution Test, Kruskal-Wallis and Mann-Whitney Tests were applied for statistical analysis in the study. All of the tested extracts, isopropanolic extracts of marjoram leaves showed the best inhibition zones against Klebsiella pneumoniae CCM 2318 (24 mm) and Candida albicans ATCC 10239 (28 mm), methanolic extracts against Staphylococcus aureus ATCC 6538/P (26 mm). In our study, the results of the inhibition zones are varied 6 to 26 mm against tested bacteria. The results of Mann-Whitney Tests showed that isopropanolic extract is a significant different from chloroform, water and hexane extracts, statistically (Pisopropanolic-chloroform=0.003, Pisopropanolic-water=0.000, Pisopropanolic-hexane=0.000). Isopropanolic extracts of O.marjorana were among the most active with the MIC values ranging from 0.008-64 mg/mL. MICs of O.marjorana L. different extracts obtained by the broth serial dilution method. The GC/MS analyses allowed fifteen compounds to be determined; the main constituents of the of isopropanolic extract of marjoram leaves was carvacrol %86.33. Carvacrol, major constituent of the marjoram isopropanolic leaf extract in our study, had possible antimicrobial activity by testing commercial carvacrol. It has been observed that the extracts can be used in the food industry to inhibit the growth of pathogens or as natural preservatives in foods. 

References

  • Adams RP.(1995). Identification of essential oil components by gas chromatography/masss spectrometry. Allured Publishing: Carol Stream, IL,.
  • Adwan G, Mhanna, M. (2009). Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Asian Pacific Journal of Tropical Medicine,2: 3, 46-51.
  • Ates DA, Erdogrul OT.(2003). Antimicrobial activity of various medicinal and commercial plants extracts. Turkish Journal of Biology, 27: 157-162. Retrieved from https://dergipark.org.tr/tr/pub/tbtkbiology/issue/11734/140074
  • Baser K.H.C, N. Kirimer and G. Tümen.(2014). Composition of the essential oil of Origanum majorana L. from Turkey. Journal of Essential Oil Research,5,577-579. Doi: https://doi.org/10.1080/10412905.1993.9698283
  • Baydar H, Sagdic O, Ozkan G, Karadogan T.(2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control, 15,169–72. Doi: https://doi.org/10.1016/S0956-7135(03)00028-8
  • Ben A.A., Combes S, Preziosi-Belloy L, Gontard N, Chalier P.(2006). Antimicrobial activity of carvacrol related to its chemical structure. Letters in Applied Microbiology,.Aug43(2):149-54. Doi: https://doi.org/10.1111/j.1472-765X.2006.01938.x
  • Ben, H. E. N., M. M. Abdelkefi, R. B. Aissa and M. M. Chabouni.(2001). Antibacterial screening of Origanum majorana L. oil from Tunisia. Journal of Essential Oil Research, 13, 295-297. Bnyan I.A., Aumaima
  • Tariq Abid, Hamid Naji Obied.(2014). Antibacterial Activity of Carvacrol against Different Types of. Bacteria. Journal of Natural Sciences Research, 4(9), 13-16.
  • Burt S.(2000). Essential oils: their antibacterial properties andpotential applications in foods - a review. International Journal of Food Microbiology, 94, 223-253. Doi: https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  • Busatta C, Vidal R. S, Popiolski A. S, Mossi A. J, Dariva C, Rodrigues M. R. A, Corazza F. C, Corazza M. L, Oliveira J. V & Cansian, R. L. (2008). Application of (Origanum majorana L.) essential oil as an antimicrobial agent in sausage. Food Microbiology, 25(1), 207-211. Doi: https://doi.org/10.1016/j.fm.2007.07.003
  • Daferea D. J, Ziogas, B. N. and Polissiou, M. G.(2000). GC-MS analysis of essential oils from some Greek aromatic plants and their fungitoxicity on Penicillium digitatum. Journal of Agriculture Food Chemistry, 48, 2576-2581. Doi: https://doi.org/10.1021/jf990835x
  • Deans SG, and Svoboda KP. (1990). The antimicrobial properties of Marjoram (Origanum majorana L.) volatile oil. Flavour and Fragrance Journal; 5, 187–190. Doi: https://doi.org/10.1002/ffj.2730050311
  • Dorman HJD, Deans SG.(2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology, 88,308-316. Doi: http://dx.doi.org/10.1046/j.1365-2672.2000.00969.x
  • Ezzeddine N.B, Abdelkafi M.M, Ben Aissa R & Chaabouni, M.M.(2001). Antibacterial screening of (Origanum majorana L.), oil from Tunisia. Journal of Essential Oil Research,13,295-297.
  • Farooqi A. A. and B. S. Sreeramu.(2004). Cultivation of medicinal and aromatic crops. Universities Press, India pp. 465-470. Doi: https://doi.org/10.1080/10412905.2001.9699698
  • Hammer KA, Carson CF, Riley TV. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbioogyl, 86,985–90. Doi: https://doi.org/10.1046/j.1365-2672.1999.00780.x
  • Helal G. A, Sarhan M. M, Abu Shahla A. N. K, Abou El-Khair E. K.(2006). Antimicrobial activity of some essential oils against microorganisms deteriorating fruit juices. Mycobiology, 34(4), 219-229. Doi: https://dx.doi.org/10.4489%2FMYCO.2006.34.4.219
  • Holley R.A, Patel D.(2005). Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiology, 22, 273-292. Doi: https://doi.org/10.1016/j.fm.2004.08.006
  • Janssen A. M, Scheffer, J. J. C. and Baerheim-Svendeaen A. (1987). Antimicrobial activity of essential oils: a 1976-86 literature review. Aspects of the test methods. Doi: https://doi.org/10.1007/BF02029329
  • Kim K.J, Yu, H.H., Cha J.D, Seo S.J, Choi, N.Y., You, YO.(2005) Antibacterial activity of Curcuma longa L. against methicillin-resistant Staphylococcus aureus. Phytotheraphy Research, 19(7),599-604. Doi: https://doi.org/10.1002/ptr.1660
  • Kirtikar K. R. and B. D. Basu.(1985). Indian medicinal plants. (Eds.: E. Blatter, J.F.Caius and S.K. Mhaskar). Bishen Singh and Mahendra Pal Sing,Dehradun. 1985; Vol. 3, pp. 250.
  • Lambert RJW, Skandamis PN, Coote PJ, Nychas GJE.(2001). A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology,91,453–62. Doi: https://doi.org/10.1046/j.1365-2672.2001.01428.x
  • Leeja L& Thoppil J.E.(2007). Antimicrobial activity of methanol extract of (Origanum majorana L.) (sweet marjoram). Journal of Environmental Biology, 28 (1),145-146.
  • Mishra, A. K. and Dubey, N. K. (1994). Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Applied and Environmental. Microbiology, 60, 1101-1105.
  • Mohamed N, Yasmen SH, Nohir G. (2011). Antimicrobial activity of water and ethanol Marjoram (Origanum marjorana L.) extract. The 6th Arab and 3rd International Annual Scientific Conference, 2350-2366.
  • Novak J, F. Pank, J. Langbehn, W.D. Blüthner, C. Vender, L.V.Niekerk, W. Junghanns, C. Franz.(2004). Determination of growing location of marjoram (Origanum majorana L.) samples by comparison of essential oil profiles. Flavour Fragrance Journal, 19, 263-267.
  • Ozdemir G, Karabay NU, Conk Dalay M, Pazarbası B.(2004). Antimicrobial activity of volatile component and various extracts of Spirulina plantensis. Phototherapy Research, 18,754-757. Doi: https://doi.org/10.1002/ptr.1541
  • Pernin A, Guillier L, Dubois-Brissonnet F.(2019). Inhibitory activity of phenolic acids against Listeria monocytogenes: deciphering the mechanisms of action using three different models.  Food Microbiology,  80,18–24. Doi: https://doi.org/10.1016/j.fm.2018.12.010
  • Phadke SA and Kulkarni SD.(1989). Screening of in vitro antibacterial activity of Terminalia chebula, Eclapta alba and Ocimum sanctum. Indian Journal of Medical Sciences,43(5), 113-117.
  • Pino J.A., A. Rosado, M. Estarron, V. Fuentes.(1997). Essential oil of Marjoram (Origanum majorana L.) grown in Cuba. Journal of Essential Oil Research,9, 481-482.
  • Prudent D, Perineau F, Bessiere J. M, Michel, G. M. and Baccou,J. C(1995). Analysis of the essential oil of wild oregano form Martinique (Coleus aromaticus Benth.). Evaluation of its bacteriostatic and fungistatic properties. J. Essent. Oil Res, 7,165-173. Doi: https://doi.org/10.1080/10412905.1995.9698492
  • Ramos S, Luis B. Rojas, Maria Eugenia Lucena, Gina Meccia & Alfredo Usubillaga. (2011). Chemical composition and antibacterial activity of Origanum majorana L. essential oil from the Venezuelan Andes, Journal of Essential Oil Research,23:5,45-49. Doi: https://doi.org/10.1080/10412905.2011.9700481
  • Selim S.A., Mohamed H. Abdel Aziz, Mona S. Mashait and Mona F. Warrad.(2013). Antibacterial activities, chemical constitutes and acute toxicity of Egyptian Origanum majorana L., Peganum harmala L. and Salvia officinalis L. essential oils. African Journal of Pharmcy and Pharmacology,7(13), 725-735. Doi: http://dx.doi.org/10.5897/AJPP2013.3518
  • Shu C. K. and Lawrence, B. M.(1997). Reasons for the variation in composition of some commercial essential oils. pp 138-159.In: S. J. Risch and C. T. Ho. Eds. ACS symposium series: Col.660. Spices, Flavor chemistry and antioxidant properties.
  • Vági E, B. Simándi, A. Suhajda, E. Héthelyi.(2005). Essential oil composition and antimicrobial activity of Origanum majorana L. extracts obtained with ethyl alcohol and supercritical carbon dioxide. Food Research International, 38, 51-57. Doi: http://dx.doi.org/10.1016/j.foodres.2004.07.006
  • Vardar-Ünlü G, Candan F, Sokmen A, Daferera D, PolissiouM, Sokmen M, Donmez, E. and Tepe B. (2003). Antibacterial and antioxidant activity of the essential oil and methanol extracts of Thymus pectinatus Fisch. et Mey var. Pectinatus (Lamiaceae). Journal of Agriculture and Food Chemistry, 51, 63-67. Doi: https://doi.org/10.1021/jf025753e

Details

Primary Language English
Subjects Food Science and Technology
Published Date September 2021
Journal Section Research Articles
Authors

Nur CEYHAN GÜVENSEN (Primary Author)
EGE UNIVERSITY, TIRE VOCATIONAL SCHOOL
0000-0001-8753-2664
Türkiye


Dilek KESKİN
ADNAN MENDERES UNIVERSITY, KÖŞK VOCATIONAL SCHOOL
0000-0003-2479-0070
Türkiye

Publication Date September 15, 2021
Application Date March 29, 2021
Acceptance Date July 9, 2021
Published in Issue Year 2021, Volume 5, Issue 3

Cite

APA Ceyhan Güvensen, N. & Keskin, D. (2021). Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey . International Journal of Agriculture Environment and Food Sciences , 5 (3) , 398-404 . DOI: 10.31015/jaefs.2021.3.19