In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L.

Year 2022, Volume: 6 Issue: 3, 389 - 395, 23.09.2022

Aybeniz Yıldırım Ali Şen Mayram Tuysuz Ayşe Seher Birteksöz Tan İsmail Şenkardeş Leyla Bitiş

https://doi.org/10.31015/jaefs.2022.3.7

Cited By: 1

Abstract

In this study, in vitro biological activities and total phenol/flavonoid contents of methanol extract (ISM) and its hexane (ISH), chloroform (ISC), ethyl acetate (ISEA) and aqueous methanol (ISAM) fractions obtained from aerial parts of Inula salicina were investigated. ISEA showed the highest antioxidant activity against DPPH and ABTS radicals with an IC50 value of 0.014 mg ml-1for both assays. ISEA exhibited a good anti-inflammatory activity with an IC50 value of 0.060 mg ml-1. ISEA was found to exhibit a moderate level of antidiabetic activity against α amylase enzyme with an IC50 value of 0.290 mg ml-1. ISEA and ISM presented low and moderate inhibitory activity against acetylcholinesterase and butyrylcholinesterase enzymes with IC50 values of 0.577 and 0.279 mg ml-1, respectively. ISC with MIC values of 78 and 156 µg ml-1 displayed a significant antimicrobial activity against Staphylococcus aureus and S. epidermidis, respectively. Almost all extracts had moderate effect against Candida species. The highest total phenolic and flavonoid contents were determined in ISEA with 574.8 mg GAE (gallic acid equivalent) g-1 extract and 30.48 mg QE (quercetin equivalent) g-1 extract, respectively. These results showed that ISEA had a good antioxidant and anti-inflammatory activity with moderate α-amylase and butyrylcholinesterase inhibitory activity. Also, ISC exhibited a significant antimicrobial activity against Staphylococcus species.

Keywords

Asteraceae, Biological activity, Extracts, Fractions, Inula salicina

References

• Abuhamdah, S., Abuhamdah, R., Shakya, A., Al-Olimat, S. M., Chazot, P. (2014). Neuropharmacological evaluation of selected Jordanian traditional herbal medicines. African Journal of Pharmacy and Pharmacology, 8(48), 1235-1241. Doi: https://doi.org/10.5897/AJPP2014.4097
• Anonymous, (2021). Bizim bitkiler. Retrieved from https://www.bizimbitkiler.org.tr/v2/hiyerarsi.php?c=Inula. Access date: 29.04.2021 [in Turkish]
• Berk, S., Tepe, B., Arslan, S. (2000). Screening of the antioxidant, antimicrobial and DNA damage protection potentials of the aqueous extract of Inula oculus-christi. African Journal of Pharmacy and Pharmacology, 5(14), 1695-1702. Retrieved from https://academicjournals.org/journal/AJPP/article-abstract/62A2AE730444
• Bitis, L., Sen, A., Ozsoy, N., Birteksoz-Tan, S., Kultur, S., Melikoglu, G. (2017). Flavonoids and biological activities of various extracts from Rosa sempervirens leaves. Biotechnology & Biotechnological Equipment, 31(2), 299-303. Doi: https://doi.org/10.1080/13102818.2016.1277956
• Brahmi-Chendouh, N., Piccolella, S., Crescente, G., Pacificoa, F., Boulekbache, L., Hamri-Zeghichi, S., Akkal, S., Madani, K., Pacificoa, S. (2019). A nutraceutical extract from Inula viscosa leaves: UHPLC-HR-MS/MS based polyphenol profile and antioxidant and cytotoxic activities. Journal of Food and Drug Analysis, 27(3), 692-702. Doi: https://doi.org/10.1016/j.jfda.2018.11.006
• Bucchini, A., Ricci, D., Messina, F., Marcotullio, M. C., Curini, M., Giampe, L. (2015). Antioxidant and antifungal activity of different extracts obtained from aerial parts of Inula crithmoides L. Natural Product Research, 29(12), 1173–1176. Doi: https://doi.org/10.1080/14786419.2014.983102
• Cafarchia, C., De Laurentis, N., Milillo, M. A., Losacco, V., Puccini, V. (2002). Antifungal activity of essential oils from leaves and flowers of Inula viscosa (Asteraceae) by Apulian region. Parassitologia, 44, 153-156. Retrieved from https://pubmed.ncbi.nlm.nih.gov/12701377/
• Clinical and Laboratory Standards Institute (CLSI) (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: Approved Standard M7-A7, CLSI, Wayne, Pennsylvania.
• Clinical and Laboratory Standards Institute (CLSI) (2008). Reference method for broth dilution antifungal susceptbility testing of yeasts; Approved Standart M27-A3, CLSI, Wayne, Pennsylvania.
• Clinical and Laboratory Standarts Institute (2014) Performance standarts for antimicrobial susceptibility testing; 24th informational supplement. M 100-S24, CLSI, Wayne, Pennsylvania.
• Davis, P. H. (1975). Flora of Turkey and The East Aegean Islands. Volume 5, Edinburgh University Press, Edinburgh
• Deriu, A., Zanetti, S., Sechi, L. A., Marongiu, B., Piras, A., Porcedda, S., Tuveri, E. (2008). Antimicrobial activity of Inula helenium L. essential oil against Gram-positive and Gram-negative bacteria and Candida spp. International Journal of Antimicrobial Agents, 31, 581–592. Doi: https://doi.org/10.1016/j.ijantimicag.2008.02.006
• Dorn, D. C., Alexenizer, M., Hengstler, J. G., Dorn, A. (2006). Tumor cell specific toxicity of Inula helenium extracts. Phytotherapy Research, 20, 970-980. Doi: https://doi.org/10.1002/ptr.1991
• Gao, X., Ohlander, M., Jeppsson, N., Björk, L., Trajkovski, V. (2000). Changes in Antioxidant Effects and Their Relationship to Phytonutrients in Fruits of Sea Buckthorn (Hippophae rhamnoides L.) during Maturation. Journal of Agricultural and Food Chemistry, 48, 1485-1490. Doi: https://doi.org/10.1021/jf991072g
• Gokbulut, A., Gunal, S., Sarer, E. (2016). Antioxidant and Antimicrobial Activities and Phenolic Compounds of Inula peacockiana and Inula thapsoides ssp. thapsoides. Chemistry of Natural Compounds, 52, 119-122. Doi: https://doi.org/10.1007/s10600-016-1564-0
• Gökbulut, A., Özhana, O., Satılmiş, B., Batçioğlu, K., Günal, S., Şarer, E. (2013). Antioxidant and antimicrobial activities, and phenolic compounds of selected Inula species from Turkey. Natural Product Communications, 8(4), 475-478. Doi: https://doi.org/10.1177/1934578X1300800417
• Im, K. H., Nguyen, T. K., Choi, J., and Lee, T. S. (2016). In vitro antioxidant, anti-diabetes, anti-dementia, and inflammation inhibitory effect of Trametes pubescens fruiting body extracts. Molecules, 21(5), 639. Doi: https://doi.org/10.3390/molecules21050639
• Ivanova, V., Trendafilova, A., Todorova, M., Danova, K., Dimitrov, D. (2017). Phytochemical profile of Inula britannica from Bulgaria. Natural Product Communications, 12(2), 153-154. Doi: https://doi.org/10.1177/1934578X1701200201
• İduğ, T., Hızlı Güldemir, H. , Şen, A., Güldemir, O. (2022). Investigation on the effects of cooking methods on anti-inflammatory and antioxidant activities of five mostly consumed vegetables in winter. International Journal of Agriculture Environment and Food Sciences, 6 (1), 182-188 . Doi: https://doi.org/10.31015/jaefs.2022.1.23
• Jallali, I., Zaouali, Y., Missaoui, I., Smeoui, A., Abdelly, C., Ksouri, R. (2014). Variability of antioxidant and antibacterial effects of essential oils and acetonic extracts of two edible halophytes: Crithmum maritimum L. and Inula crithmoїdes L. Food Chemistry, 145(15), 1031-1038. Doi: https://doi.org/10.1016/j.foodchem.2013.09.034
• Kim, S. H., Kwon, C. S., Lee, J. S., Son, K. H., Lim, J. K., and Him, J. S. (2002). Inhibition of carbohydrate - digesting enzymes and amelioration of glucose tolerance by Korean medicinal herbs. Journal of Food Science and Nutrition, 7(1), 62-66. Doi: https://doi.org/10.3746/jfn.2002.7.1.062
• Konıshı, T., Shımada, Y., Nagao, T., Okabe, H., Konoshıma, T. (2002). Antiproliferative sesquiterpene lactones from the roots of Inula helenium. Biological and Pharmaceutical Bulletin, 25(10), 1370-1372. Doi: https://doi.org/10.1248/bpb.25.1370
• Mahmoudı, H., Hosnı, K., Zaoualı, W., Amrı, I., Zargounı, H., Hamıda, N. B., Kaddour, R., Hamrounı, L., Nasrı, M. B., Ouerghı, Z. (2016). Comprehensive phytochemıcal analysis, antioxidant and antifungal activities of Inula viscosa Aiton leaves. Journal of Food Safety, 36, 77–88. Doi: https://doi.org/10.1111/jfs.12215
• Orhan, N., Gökbulut, A., Orhan, D. D. (2017). Antioxidant potential and carbohydrate digestive enzyme inhibitory effects of five Inula species and their major compounds. South African Journal of Botany, 111, 86–92. Doi: https://doi.org/10.1016/j.sajb.2017.03.040
• Phosrithong, N., Nuchtavorn, N. (2016). Antioxidant and anti-inflammatory activites of Clerodendrum leaf extracts collected in Thailand. European Journal of Integrative Medicine, 8(3), 281-285. Doi: https://doi.org/10.1016/j.eujim.2015.10.002
• Ramakrishna, R., Sarkar, D., Schwarz, P., Shetty, K. (2017). Phenolic linked anti-hyperglycemic bioactives of barley (Hordeum vulgare L.) cultivars as nutraceuticals targeting type 2 diabetes. Industrial Crops and Products, 107, 509-517. Doi: https://doi.org/10.1016/j.indcrop.2017.03.033
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. Doi: https://doi.org/10.1016/S0891-5849(98)00315-3
• Saraiva, A. M., Castro, R. H. A., Cordeiro, R. P., Peixoto Sobrinho, T. J. S., Castro, V. T. N. A., Amorim, E. L. C., Xavier, H. S., Pisciottano, M. N. C. (2011). In vitro evaluation of antioxidant, antimicrobial and toxicity properties of extracts of Schinopsis brasiliensi Engl. (Anacardiaceae). African Journal of Pharmacy and Pharmacology, 5(14), 1724–1731. Doi: https://doi.org/10.5897/AJPP11.428
• See, I., Ee, G. C. L., Mah, S. H., Jong, V. Y. M., Teh, S. S. (2017). Effect of solvents on phytochemical concentrations and antioxidant activity of Garcinia benthamiana stem bark extracts. Journal of Herbs, Spices & Medicinal Plants, 23, 117-127. Doi: https://doi.org/10.1080/10496475.2016.1272523
• Sen, A., Kurkcuoglu, M., Senkardes, I., Bitis, L., Baser, K. H. C. (2019). Chemical Composition, Antidiabetic, Anti-inflammatory and Antioxidant Activity of Inula ensifolia L. Essential Oil. Journal of Essential Oil Bearing Plants 22(4), 1048-1057. Doi: https://doi.org/10.1080/0972060X.2019.1662333
• Sevindik, E., Aydın, S., Paksoy, M. Y., Sokmen, B. B. (2020). Anti-Urease, total phenolic content and antioxidant activities of some Inula L. (Asteraceae) taxa in Turkey. Genetika, 52(3), 825-834. Doi: https://doi.org/10.2298/GENSR2003825S
• Stojanović-Radić, Z., Čomić, L., Radulović, N., Blagojević, P., Denić, M., Miltojević, A., Rajković, J., Mihajilov-Krstev, T. (2012). Antistaphylococcal activity of Inula helenium L. root essential oil: Eudesmane sesquiterpene lactones induce cell membrane damage. European Journal of Clinical Microbiology & Infectious Diseases, 31, 1015-1025. Doi: https://doi.org/10.1007/s10096-011-1400-1
• Tavares, W. R., Seca, A. M. L. (2019). Inula L. secondary metabolites against oxidative stress-related human diseases. Antioxidants, 8, 122. Doi: https://doi.org/10.3390/antiox8050122
• Tardio, J., Pardo-de-Santayana, M., Morales, R. (2006). Ethnobotanical review of wild edible plants in Spain. Botanical Journal of the Linnean Society, 152: 27-71. Doi: https://doi.org/10.1111/j.1095-8339.2006.00549.x
• Trendafilova, A., Ivanova, V., Rangelov, M., Todorova, M., Ozek, G., Yur, S., Ozek, T., Aneva, I., Veleva, R., Moskova-Doumanova, V., Doumanov, J., Topouzova-Hristovae, T. (2020). Caffeoylquinic acids, cytotoxic, antioxidant, acetylcholinesteraseand tyrosinase enzyme inhibitory activities of six Inula species from Bulgaria. Chemistry Biodiversity, 17(4), e2000051. Doi: https://doi.org/10.1002/cbdv.202000051
• Trendafilova, A., Todorova, M., Ozek, T., Ozek, G., Aneva, I. (2020). Volatile constituents of four Inula species of Bulgarian origin. Biochemical Systematics and Ecology, 90, 104035. Doi: https://doi.org/10.1016/j.bse.2020.104035
• Yıldırım, A., Şen, A., Doğan, A., Bitis, L. (2019). Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson. International Journal of Secondary Metabolite, 6(2), 211-222. Doi: https://doi.org/10.21448/ijsm.510316
• Zhang, R., Zeng, Q., Deng, Y., Zhang, M., Wei, Z., Zhang, Y., Tang, X. (2013). Phenolic profiles and antioxidant activity of litchipulp of different cultivars cultivated in Southern China. Food Chemistry, 136, 1169–1176. Doi: https://doi.org/10.1016/j.foodchem.2012.09.085
• Zou, Y., Chang, S. K., Gu, Y., Qian, S. Y. (2011). Antioxidant activity and phenolic compositions of lentil (Lens culinaris var. morton) extract and its fractions. Journal of Agricultural and Food Chemistry, 59(6), 2268-2276. Doi: https://doi.org/10.1021/jf104640k