A promising method for selecting imazamox-resistant sunflower plants

Year 2024, Volume: 8 Issue: 2, 479 - 485, 27.06.2024

Pınar Harmancı , Elif Yaman , Mehmet Demir Kaya

https://doi.org/10.31015/jaefs.2024.2.21

Abstract

This study was conducted to investigate the potential of using the germination test as a model for screening imazamox resistance in sunflower plants . A standard germination test was performed by applying different doses of imazamox (control, 0.1, 0.2, 0.4, 0.8, and 1.6 mM) to imidazoline-resistant (IMI-R) and susceptible (IMI-S) sunflower hybrids. Germination percentage, mean germination time, germination index, shoot length, root length, seedling fresh and dry weight, and phytotoxicity percentage for seedling growth parameters were investigated. The results showed that imazamox concentrations slightly affected the germination percentage of two sunflower hybrids at only 1.6 mM. Increasing doses of imazamox led to an increase in the mean germination time and a decrease in the germination index for both sunflower hybrids, following a similar trend. Seedling growth parameters such as shoot length, root length, and seedling fresh weight were significantly decreased by increasing imazamox doses. In addition, the differences between IMI-R and IMI-S sunflower hybrids were very evident for these parameters. The IMI-S sunflower hybrid showed sensitivity in the presence of imazamox, while no changes in the IMI-R hybrid were determined up to 0.4 mM. The inhibition percentage was higher in the IMI-S hybrid than in the IMI-R. It was concluded that the optimal dose of imazamox for the selection of resistant plants during the early growth stage was found to be 0.2 mM. The germination parameters were not good criteria for imazamox resistance, while root length, shoot length, and seedling fresh weight should be considered as selection criteria for resistance to imazamox in sunflower.

Keywords

Helianthus annuus L., Herbicide, Resistant, Germination, Root length

Thanks

The author is thankful to Ph.D. student A. Onrat for their kind help during the measurement of investigated parameters in the study.

References

• Archana & Pandey, N. (2016). Physiological and biochemical effects of boron toxicity in mustard during the seedling stage. Journal of Plant Nutrition, 39(6), 820-827. https://doi.org/10.1080/01904167.2015.1047523.
• Breccia, G., Bisio, M.B., Picardi, L. & Nestares, G., (2018). A rapid phenotyping method for imazamox resistance in wheat. Planta Daninha 36, 1–8. https://doi.org/10.1590/S0100-83582018360100142.
• Breccia, G., Picardi, L. & Nestares, G. (2020). Cultivar variation for imazamox resistance in wheat (Triticum aestivum L.): Insights into enzymatic assays for early selection. Plant Physiology and Biochemistry, 151, 438-442. https://doi.org/10.1016/j.plaphy.2020.03.045.
• Fernández-Martínez, J., Domínguez J., Pérez-Vich, B. & Velasco, L. (2009). Current research strategies for sunflower broomrape control in Spain. Helia, 32(51), 47–56. https://doi.org/10.2298/hel0951047f.
• Haliloğlu, K., Türkoğlu, A. & Aydin, M. (2022). Determination of imazamox herbicide dose in in vivo selection in wheat (Triticum aestivum L.). Eregli journal of Agricultural Science. http://dx.doi.org/10.54498/etbd.2022.6.
• Harrison, G.W., Weber, J.B. & Baird, J.V. (1976). Herbicide phytotoxicity as affected by selected properties of North Carolina soils. Weed Science, 24(1), 120-126. https://doi.org/10.1017/S0043174500065528.
• ISTA (2018). International rules for seed testing. International Seed Testing Association. Bassersdorf, Switzerland.
• Kaya, M.D. & Kolsarıcı, Ö. (2005). A new goal in sunflower breeding: Resistance to imidazolinone. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 9(2), 69-72.
• Malidza, G., Jocic, S. & Skoric, D. (2003). Weed and broomrape (O. cernua) control in Clearfield in Sunflower. Proceeding of European Weed Research Society 7th Mediterranean Symposium. 6-9 May. Cukurova University, Adana, Turkey, pp. 51–52.
• Mitkov, A., Yanev, M., Neshev, N., Tonev, T., Joita-Pacureanu, M. & Cojacaru, F. (2019). Efficacy against broomrape and selectivity of imazamox-containing herbicides in sunflower. Romanian Agricultural Research, 36, 201-207.
• Neshev, N., Yanev, M., Mitkov, A. & Tonev, T. (2020). Efficacy and selectivity of imazamox-containing herbicides at Clearfield® and Clearfield® plus sunflower hybrids. Scientific Papers Series A. Agronomy, 63(1), 450-457.
• Salehzade H., Shishvan M.I., Ghiyasi M., Forouzin F. & Siyahjani A.A. (2009). Effect of seed priming on germination and seedling growth of wheat (Triticum aestivum L.). Research Journal of Biological Sciences, 4, 629-631.
• Shaner, D.L., Stidham, M., Singh, B. & Tan, S. (2019). Imidazolinones herbicides. In: Peter, Y., Jeschke, P., Witschel, M., Krämer, W. & Schirmer, U. (Eds.), Modern Crop Protection Compounds. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, pp. 95–106. https://doi.org/10.1002/9783527699261.ch2.
• Simic, M., Dragicevic, V., Knezevic, S., Radosavljevic, M., Dolijanovic, Z. & Filipovic, M. (2011). Effects of applied herbicides on crop productivity and on weed infestation in different growth stages of sunflower (Helianthus annuus L.). Helia, 34, 27–38. https://doi.org/10.2298/hel1154027s.
• Stefanic, E., Rasic, S., Lucic, P., Zimmer, D., Mijic, A., Antunovic, S., ... & Stefanic, I. (2023). The critical period of weed control influences Sunflower (Helianthus annuus L.) yield, yield components but not oil content. Agronomy, 13(8), 2008. https://doi.org/10.3390/agronomy13082008.