Use of the radicle emergence test (RE) to estimate germination and emergence potential in sponge gourd (Luffa aegyptiaca Mill.) seed genotypes

Year 2024, Volume: 8 Issue: 4, 779 - 785

Kazım Mavi , Ahmet Hakan Eker , İbrahim Demir

https://doi.org/10.31015/jaefs.2024.4.6

Abstract

The study was carried out to test whether the radicle emergence (RE) test could be used to rank 12 Luffa (Luffa aegyptiaca Mill.) seed genotypes according to total (TG, %) and normal (NG, %) germination percentages in laboratory conditions (25°C, 14 days, dark), and mean germination time (MGT, day), seedling emergence percentage (SE, %), seedling shoot weight (SSW, g/plant) and hypocotyl thickness (HT, mm/plant) in climatic room conditions (23±2°C, 70% relative humidity), in peat moss medium after 15 days. RE (2 mm radicle) counts were made at 24, 48, 72, 96, 120 and 144 hours at 25°C in the dark after germination was set up. RE values were correlated to seed germination and seedling quality parameters. The highest correlation values occurred after 72h and onwards. RE 72h and seed quality parameters were regressed and were found to be significantly related to TG (R2=0.732, P<0.001), NG (R2=0.751, P<0.001), MGT (R2=0.842, P<0.001), SE (R2=0.754, P<0.001), SSW (R2=0.349, P<0.05), and HT (R2=0.757, P<0.001). The potential of the RE test to discriminate between Luffa genotypes according to their germination and emergence potential is discussed.

Keywords

Luffa genotypes, RE test, Germination, Seedling emergence, Seed vigour

References

• Demir, I., Ermis, S., Mavi, K. & Matthews, S. (2008). Mean germination time of pepper seed lots (Capsicum annuum L.) predicts size and uniformity of seedlings in germination tests and transplant modules. Seed Science and Technology, 36(1), 21-30.
• Demir, I., Özden, E., Gökdaş, Z., Njie, E. S. & Aydin, M. (2020). Radicle emergence test predicts normal germination percentages of onion seed lots with different cultivars and genotypes. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 25(3), 434-442.
• Eren, E., Ermis, S., Oktem, G. & Demir, I. (2023). Seed longevity potential predicted by radicle emergence (RE) vigor test in watermelon seed cultivars. Horticulturae, 9(2), 280.
• Ermis, S., Oktem, G., Mavi, K., Hay, F. R. & Demir, I. (2022). The radicle emergence test and storage longevity of cucurbit rootstock seed lots. Seed Science and Technology, 50(1), 1-10.
• ISTA (2022). International Rules for Seed Testing, International Seed Testing Association, Bassersdorf, Switzerland.
• Jang, Y., Moon, J. H., Kim, S. G., Kim, T., Lee, O. J., Lee, H. J. & Wi, S. H. (2022). Effect of low-temperature tolerant rootstocks on the growth and fruit quality of watermelon in semi-forcing and retarding culture. Agronomy, 13(1), 67.
• Khajeh-Hosseini, M., Lomholt, A. & Matthews, S. (2009). Mean germination time in the laboratory estimates the relative vigour and field performance of commercial seed lots of maize (Zea mays L.). Seed Science and Technology, 37(2), 446-456.
• Khajeh-Hosseini, M, Nasehzadeh, M. & Matthews, S. (2010). Rate of physiological germination relates to the percentage normal seedlings in standard germination tests of naturally aged seed lots of oilseed rape. Seed Science and Technology 38 (3), 602-611
• Lee, J. M., Kubota, C., Tsao, S. J., Bie, Z., Echevarria, P. H., Morra, L. & Oda, M. (2010). Current status of vegetable grafting: diffusion, grafting techniques, automation. Scientia Horticulturea, 127, 93-105. https://doi.org/10.1016/j.scienta.2010.08.003
• Li, H., Wang, F., Chen, X. J., Shi, K., Xia, X. J., Considine, M. J., Yu, J. Q. & Zhou, Y. H. (2014). The sub/supra-optimal temperature-induced inhibition of photosynthesis and oxidative damage in cucumber leaves are alleviated by grafting onto figleaf gourd/luffa rootstocks. Physiologia Plantarum, 152, 571–584.
• Marr, K. L., Bhattarai, N. K. & Xia, Y. M. (2005) Allozymic, morphological, and phenological diversity in cultivated Luffa acutangula (Cucurbitaceae) from China, Laos, and Nepal, and allozyme divergence between L. acutangula and L. aegyptiaca. Economic Botany, 59, 154–165.
• Matthews, S. & Khajeh Hosseini, M. (2006). Mean germination time as an indicator of emergence performance in soil of seed lots of maize (Zea mays). Seed Science and Technology, 34(2), 339-347.
• Matthews, S., Beltrami, E., El-Khadem, R., Khajeh-Hosseini, M., Nasehzadeh, M. & Urso, G. (2011). Evidence that time for repair during early germination leads to vigour differences in maize. Seed Science and Technology, 39(2), 501-509.
• Matthews, S., Wagner, M. H., Kerr, L. & Powell, A. A. (2018). Potential for early counts of radicle emergence and leakage of electrolytes as quick tests to predict the percentage of normal seedlings. Seed Science and Technology, 46(1), 1-18.
• Matthews, S., Wagner, M. H., Kerr, L., McLaren, G. & Powell, A. A. (2012). Automated determination of germination time courses by image capture and early counts of radicle emergence lead to a new vigour test for winter oilseed rape (Brassica napus). Seed Science and Technology, 40(3), 413-424.
• Mavi, K. & Demir, I. (2007). Controlled deterioration and accelerated aging tests predict relative seedling emergence potential of melon seed lots. HortScience, 42(6), 1431-1435.
• Mavi, K., Demir, I. & Matthews, S. (2010). Mean germination time estimates the relative emergence of seed lots of three cucurbit crops under stress conditions. Seed Science and Technology, 38(1), 14-25.
• Mavi, K., Ermis, S. & Demir, I. (2006). The effect of priming on tomato rootstock seeds in relation to seedling growth. Asian Journal of Plant Sciences, 5(6), 940-947.
• Mavi, K., Gündüz, K., Uzunoğlu, F. & Karaat, F. E. (2021). Morphological characterization of sponge gourd (Luffa aegyptiaca Mill.) genotypes from the eastern mediterranean region of Turkey. Genetika, 53(3), 1043-1064.
• Mavi, K., Gündüz, K., Yıldırım, D. & Uzunoğlu, F. (2018). Seed characteristics diversity in sponge gourd (Luffa aegyptiaca Mill.) germplasms from Hatay region in Turkey. Bulletin UASVM Horticulture, 75: 40-46.
• Mavi, K., Powell, A. A. & Matthews, S. (2016). Rate of radicle emergence and leakage of electrolytes provide quick predictions of percentage normal seedlings in standard germination tests of radish (Raphanus sativus). Seed Science and Technology, 44(2), 393-409.
• Mis, S., Ermis, S., Powell, A. A. & Demir, I. (2022). Radicle emergence (RE) test identifies differences in normal germination percentages (NG) of watermelon, lettuce and carrot seed lots. Seed Science and Technology, 50(2), 257-267.
• Ozden, E., Ozdamar, C. & Demir, I. (2018). Radicle emergence test estimates predictions of percentage normal seedlings in standard germination tests of aubergine (Solanum melongena L.) seed lots. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(1), 177-182.
• Powell, A. A. & Mavi, K. (2016). Application of the radicle emergence test to radish (Raphanus sativus) seed. In Method Validation Reports 2016, pp. 65-72, International Seed Testing Association, Bassersdorf, Switzerland.
• Powell, A. A. (2022). Seed vigour in the 21st century. Seed Science and Technology, 50(2), 45-73.
• Rivard, C. L. & Louws, F. J. (2008). Grafting to manage soilborne diseases in heirloom tomato production. HortScience, 43(7), 2104-2111.
• Savvas, D., Colla, G., Rouphael, Y. & Schwarz, D. (2010). Amelioration of heavy metal and nutrient stress in fruit vegetables by grafting. Scientia Horticulturea, 127(2), 156-161.
• Schwarz, D., Rouphael, Y., Colla, G. & Venema, J. H. (2010). Grafting as a tool to improve tolerance of vegetables to abiotic stresses: thermal stress, water stress and organic pollutants. Scientia Horticulturae, 127, 162–171.
• Shinohara, T., Ducournau, S., Matthews, S., Wagner, M. H. & Powell, A. A. (2021). Early counts of radicle emergence, counted manually and by image analysis, can reveal differences in the production of normal seedlings and the vigour of seed lots of cauliflower. Seed Science and Technology, 49(3), 219-235.
• Yetisir, H., Kurt, S., Sari, N. & Tok, F. M. (2007). Rootstock potential of Turkish Lagenaria siceraria germplasm for watermelon: plant growth, graft compatibility, and resistance to Fusarium. Turkish Journal of Agriculture and Forestry, 31, 381-388.