Reduction of gossypol in cottonseed meal using halophilic archaeal fermentation for enhanced feed safety and nutritional value

Year 2024, Volume: 8 Issue: 4, 980 - 985, 28.12.2024

Fevziye Işıl Kesbiç

https://doi.org/10.31015/jaefs.2024.4.27

Abstract

This study investigated the capacity of halophilic archaea fermentation to detoxify gossypol, a poisonous polyphenolic compound present in cottonseed meal, a major protein source in animal feed. The objective of the study was to investigate the ability of the fermentation process of using halophilic archaeal fermentation to decrease the level of gossypol in cottonseed meal as well as to increase the protein content of the diets. Halophilic microorganisms are able to survive in harsh environmental conditions, offer a potential answer for difficult industrial operations. Cottonseed meal contains high quantities of gossypol, was subjected to fermentation with the halophilic archaeon Halorubrum ezzemoulense in order to reduce gossypol levels and enhance protein content. The study entailed the fermentation of cottonseed meal with H. ezzemoulense, and the amounts of gossypol in the feeds before and after fermentation were assessed using HPLC. Results showed that there was significant (p<0.05) reduction (5.59±0.17 mg/kg) in the gossypol level after microbial fermentation compared to the control which was (139.03±7.17 mg/kg). The protein and lipid content of substrate increased significantly (p<0.05). These analyses revealed modifications in the nutritional values as a result of the fermentation process. The findings indicated a substantial decrease in gossypol levels, coupled with a remarkable rise in protein content. This novel technology not only tackled the drawbacks linked to cottonseed meal but also highlighted the capacity of halophilic archaea fermentation as a sustainable and efficient technique for enhancing the nutritional value of animal feed. Further research could focus on optimizing fermentation, exploring scale-up possibilities, and evaluating broader implications for the livestock industry.

Keywords

Cottonseed meal, Fermentation, Halophiles, Archaea, Gossypol

Supporting Institution

Kastamonu University

Project Number

KUBAP01-2023-30

Thanks

The author thanks to Scientific Research Projects Committee of Kastamonu University.

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