Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions

Year 2024, Volume: 8 Issue: 4, 760 - 767

Heydem Ekinci , Necla Şaşkın , Birgül Dikmetaş , Bekir Erol Ak

https://doi.org/10.31015/jaefs.2024.4.4

Abstract

Drought stress is a significant threat to plant cultivation in arid and semi-arid regions, negatively affecting growth and leading to plant mortality. This study evaluated the in vitro drought tolerance of Aronia melanocarpa ‘Nero’ by exposing micropropagated plantlets to nutrient media containing different concentrations of PEG-8000 (0%, 1%, and 2%) during the rooting stage. Results showed that increasing PEG concentrations led to a reduction in survival, rooting, plantlet growth, and physiological parameters. The 0% PEG treatment resulted in the highest survival rate (95.83%), root number, and chlorophyll content, while the 2% PEG treatment significantly hindered these parameters. These findings indicate that Aronia melanocarpa 'Nero' is sensitive to drought stress, with reduced growth and physiological activity under higher PEG concentrations.

Keywords

Chlorophyll, Drought stress, In vitro, Micropropagation, Growth

References

• Albiski, F., Najla, S., Sanoubar, R., Alkabani, N. & Murshed, R. (2012). In vitro screening of potato lines for drought tolerance. Physiology and Molecular biology of plants, 18, 315-321. https://doi.org/10.1007/s12298-012-0127-5
• Almokar, H.M.M. & Pirlak, L. (2018). Propagation of Aronia (Aronia melanocarpa) with tissue culture. Selcuk Journal of Agriculture and Food Sciences, 32(3), 549-558. https://doi.org/10.15316/SJAFS.2018.136
• Altıncı, N.T. & Cangi, R. (2019). Drought Tolerance of Some Wine Grape Cultivars under In Vitro Conditions. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 36(2), 145-152. https://doi.org/10.13002/jafag4633
• Anjum, S.A., Xie, X.Y., Wang, L. C., Saleem, M.F., Man, C. & Lei, W. (2011). Morphological, physiological and biochemical responses of plants to drought stress. African journal of agricultural research, 6(9), 2026-2032. https://doi.org/10.5897/AJAR10.027
• Babu, G.A., Mosa Christas, K., Kowsalya, E., Ramesh, M., Sohn, S. I., & Pandian, S. (2022). Improved sterilization techniques for successful in vitro micropropagation. In Commercial Scale Tissue Culture for Horticulture and Plantation Crops (pp. 1-21). Singapore: Springer Nature Singapore.
• Bilir Ekbic, H., Gecene, İ. & Ekbic, E. (2022). Tilki Üzümlerinin (Vitis labrusca L.) Kuraklık Stresine Toleransının in vitro PEG Uygulaması ile Belirlenmesi. Erwerbs-Obstbau, 64 (Ek 1), 87-94 (in Turkish). https://doi.org/10.29278/azd.1059957
• Celik, H., Karabulut, B., & Uray, Y. (2022). Growth-Development, yield and quality characteristics of Aronia varieties grown in pots. International Journal of Innovative Approaches in Agricultural Research, 6(3), 246-254. https://doi.org/10.29329/ijiaar.2022.475.8
• Dobránszki, J., Magyar-Tábori, K., & Takács-Hudák, Á. (2003). Growth and developmental responses of potato to osmotic stress under in vitro conditions. Acta biologica hungarica, 54, 365-372.
• Ekinci, H., Saskin, N., Korkmaz, Ş., Ak, B.E. & Aydınlık, Y. (2024). The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13542-13542. https://doi.org/10.15835/nbha52113542
• Gecene, İ. (2020). Kokulu Üzümün (Vitis labrusca L.) Kuraklık Stresine Toleransının Peg Uygulamasıyla In Vitro Koşullarda Belirlenmesi. Ordu Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı, Yüksek Lisans Tezi, Ordu, Türkiye, 52 s.
• Gomez, K.A., & Gomez, A. A. (1984) Statistical procedures for agricultural research, 2nd edn. John Wiley & Sons, New York, 680 pp.
• Granato, D., Santos, J. S., Escher, G.B., Ferreira, B.L. & Maggio, R.M. (2018). Use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) for multivariate association between bioactive compounds and functional properties in foods: A critical perspective. Trends in Food Science & Technology, 72, 83-90. https://doi.org/10.1016/j.tifs.2017.12.006
• Gullapalli, R.P. & Mazzitelli, C.L. (2015). Polyethylene glycols in oral and parenteral formulations—A critical review. International Journal of Pharmaceutics, 496(2), 219-239. https://doi.org/10.1016/j.ijpharm.2015.11.015
• Hancı, F. & Cebeci, E. (2014). Investigation of proline, chlorophyll and carotenoids changes under drought stress in some onion (Allium cepa L.) cultivars. Türk Tarım ve Doğa Bilimleri Dergisi, 1(Özel Sayı-2), 1499-1504.
• Herzberger, J., Niederer, K., Pohlit, H., Seiwert, J., Worm, M., Wurm, F.R. & Frey, H. (2016). Polymerization of ethylene oxide, propylene oxide, and other alkylene oxides: synthesis, novel polymer architectures, and bioconjugation. Chemical reviews, 116(4), 2170-2243. https://doi.org/10.1021/acs.chemrev.5b00441
• Jones, H. G. & Corlett, J. E. (1992). Current topics in drought physiology. The Journal of Agricultural Science, 119(3), 291-296. https://doi.org/10.1017/S0021859600012144
• Kabay, T. & Şensoy, S. (2016). Enzyme, chlorophyl and ion changes in some common bean genotypes by drought stress. Yuzuncu Yıl University Journal of Agricultural Sciences, 26(3), 380-395.
• Kapci, B., Neradová, E., Čížková, H., Voldřich, M., Rajchl, A. & Capanoglu, E. (2013). Investigating the antioxidant potential of chokeberry (Aronia melanocarpa) products. Journal of Food and Nutrition Research, 52, 219–229.
• Kara, Z., Yazar, K., Ekinci, H., Doğan, O. & Özer, A. (2022). The Effects of Ortho Silicone Applications on the Acclimatization Process of Grapevine Rootstocks. Selcuk Journal of Agriculture and Food Sciences, 36(2), 233-237. https://doi.org/10.15316/SJAFS.2022.030
• Kocaçalışkan, İ. (2003). Bitki fizyolojisi. DPÜ Fen-Edebiyat Fakültesi Yayını.
• Lee, I.; Yang, J. (2009). Common clustering algorithms. In Comprehensive Chemometrics (pp. 577–618). Elsevier: Oxford, UK. https://doi.org/10.1016/B978-044452701-1.00064-8
• Long, S.P., ZHU, X. G., Naidu, S.L., & Ort, D.R. (2006). Can improvement in photosynthesis increase crop yields?. Plant, cell & environment, 29(3), 315-330. https://doi.org/10.1111/j.1365-3040.2005.01493.x
• Lu, H.B., Qiao, Y.M., Gong, X.C., Li, H.Q., Zhang, Q., Zhao, Z.H. & Meng, L.L. (2015). Influence of drought stress on the photosynthetic characteristics and dry matter accumulation of hybrid millet. Photosynthetica, 53(2), 306-311. https://doi.org/10.1007/s11099-015-0120-7
• Mahajan, S. & Tuteja, N. (2005). Cold, salinity and drought stresses: an overview. Archives of biochemistry and biophysics, 444(2), 139-158. https://doi.org/10.1016/j.abb.2005.10.018
• Manivannan, P., Jaleel, C. A., Sankar, B., Kishorekumar, A., Somasundaram, R., Lakshmanan, G.A. & Panneerselvam, R. (2007). Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids and Surfaces B: Biointerfaces, 59(2), 141-149. https://doi.org/10.1016/j.colsurfb.2007.05.002
• Martínez-Santos, E., Cruz-Cruz, C.A., Spinoso-Castillo, J.L. & Bello-Bello, J.J. (2021). In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress. Scientific Reports, 11(1), 22611. https://doi.org/10.1038/s41598-021-02207-0
• Mengesha, B., Mekbib, F. & Abraha, E. (2016). In vitro screening of cactus [Opuntia ficus-indicia (L.) Mill] genotypes for drought tolerance. American Journal of Plant Sciences, 7(13), 1741. https://doi.org/10.4236/ajps.2016.713163
• Mese, N. & Tangolar, S. (2019). Bazı Amerikan asma anaçlarının kurağa dayanımının in vitro’da polietilen glikol kullanılarak belirlenmesi. Yuzuncu Yıl University Journal of Agricultural Sciences, 29(3), 466-475 (in Turkish). https://doi.org/10.29133/yyutbd.559174
• Mohsen, A.T., Stino, R.G., Abd Allatif, A.M. & Zaid, N.M. (2020). In vitro evaluation of some grapevine rootstocks grown under drought stress. Plant Archives, 20(1), 1029-1034.
• Nas, Z., Esitken, A., & Pirlak, L. (2023). Determination of Plant Regeneration Protocol of “Viking” Aronia Cultivar in the In Vitro Conditions. PREPRINT (Version 1) available at Research Square, https://doi.org/10.21203/rs.3.rs-3004327/v1
• Negreanu-Pirjol, B.S., Oprea, O.C., Negreanu-Pirjol, T., Roncea, F.N., Prelipcean, A.M., Craciunescu, O., ... & Popoviciu, D.R. (2023). Health Benefits of Antioxidant Bioactive Compounds in the Fruits and Leaves of Lonicera caerulea L. and Aronia melanocarpa (Michx.) Elliot. Antioxidants, 12(4), 951. https://doi.org/10.3390/antiox12040951
• Nurcahyani, E., Sumardi, S. & Hardoko, I.Q. (2019). Analysis of chlorophyll Phalaenopsis amabilis (L.) Bl. results of the resistance to Fusarium oxysporum and drought stress. IOSR Journal of Research & Method in Education (IOSR-JRME), 12(11-I), 41-46. https://doi.org/10.9790/2380-1211014146
• Othmani, A., Ayed, S.,, Chamekh, Z., Slama-Ayed, O., Teixeira Da Silva, J.A., Rezgui, M., Slim-Amara, H.& Younes, M.B. (2021). Screening of seedlings of durum wheat (Triticum durum Desf.) cultivars for tolerance to peg-induced drought stress. Pakistan Journal of Botany, 53(3), 823-832. http://dx.doi.org/10.30848/PJB2021-3(5)
• Pham Le Khanh, H., Nemes, D., Rusznyák, Á., Ujhelyi, Z., Fehér, P., Fenyvesi, F., Váradi, J., Vecsernyés, M.& Bácskay, I. (2022). Comparative investigation of cellular effects of polyethylene glycol (PEG) derivatives. Polymers, 14(2), 279. https://doi.org/10.3390/polym14020279
• Polat, M. & Eskimez, İ. (2022). Farklı hormon kombinasyonlarının aronia (Aronia Melanocarpa (michx.) elliott) in vitro mikro çoğaltımına etkileri. Fresenius Çevre Bülteni, 31 (01A), 1219-1227 (in Turkish).
• Salehi-Lisar, S.Y., & Bakhshayeshan-Agdam, H. (2016). Drought stress in plants: causes, consequences, and tolerance. Drought stress tolerance in plants, Vol 1: physiology and biochemistry, 1-16.
• Salma, U. K., Khatun, F., Bhuiyan, M.J.H., Yasmin, S., & Khan, T.H. (2016). In vitro screening for drought tolerance of some chickpea varieties in Bangladesh. Progressive Agriculture, 27(2), 110-118.
• Saskin, N., Ak, B.E., Ekinci, H. (2022). The usage of node culture in vitro conditions. 5th International Agriculture Congress. 5-6 December, Denizli, Turkiye, pp 90-99.
• Sattar, F.A., Hamooh, B.T., Wellman, G., Ali, M.A., Shah, S.H., Anwar, Y. & Mousa, M.A.A. (2021). Growth and biochemical responses of potato cultivars under In Vitro lithium chloride and mannitol simulated salinity and drought stress. Plants, 10(5), 924. https://doi.org/10.3390/plants10050924
• Seleiman, M.F., Al-Suhaibani, N., Ali, N., Akmal, M., Alotaibi, M., Refay, Y., Dindaroglu, T., Abdul-Wajid, H.H. & Battaglia, M.L. (2021). Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants, 10(2), 259. https://doi.org/10.3390/plants10020259
• Seleiman, M.F., Refay, Y., Al-Suhaibani, N., Al-Ashkar, I., El-Hendawy, S. & Hafez, E.M. (2019). Integrative effects of rice-straw biochar and silicon on oil and seed quality, yield and physiological traits of Helianthus annuus L. grown under water deficit stress. Agronomy, 9(10), 637. https://doi.org/10.3390/agronomy9100637
• Shangguan, Z.P., Shao, M.A. & Dyckmans, J. (2000). Nitrogen nutrition and water stress effects on leaf photosynthetic gas exchange and water use efficiency in winter wheat. Environmental and experimental botany, 44(2), 141-149. https://doi.org/10.1016/S0098-8472(00)00064-2
• Sidor, A. & Gramza-Michałowska, A. (2019). Black chokeberry Aronia melanocarpa L.—A qualitative composition, phenolic profile and antioxidant potential. Molecules, 24(20), 3710. https://doi.org/10.3390/molecules24203710
• Simsek, Ö., Dönmez, D. & Kaçar, Y.A. (2018). Bazı turunçgil anaçlarının in vitro kuraklık stresi koşullarında performanslarının araştırılması. Yuzuncu Yıl University Journal of Agricultural Sciences, 28(3), 305-310 (in Turkish). https://doi.org/10.29133/yyutbd.428140
• Sivritepe, N., Erturk, U., Yerlikaya, C., Turkan, I., Bor, M. & Ozdemir, F. (2008). Response of the cherry rootstock to water stress induced in vitro. Biologia plantarum, 52, 573-576. https://doi.org/10.1007/s10535-008-0114-4
• Toosi, A.F., Baki Bakar, B.B. & Azizi, M. (2014). Effect of drought stress by using PEG 6000 on germination and early seedling growth of Brassica juncea Var. Ensabi. Scientific Papers. Series A. Agronomy, 57, 360–363.
• Vagiri, M. & Jensen, M. (2017). Influence of juice processing factors on quality of black chokeberry pomace as a future resource for colour extraction. Food chemistry, 217, 409-417. https://doi.org/10.1016/j.foodchem.2016.08.121
• Violita, V., & Azhari, S. (2021). Effect of PEG-8000 imposed drought stress on rice varieties germination. In Journal of Physics: Conference Series, 1940, 012071. https://doi.org/10.1088/1742-6596/1940/1/012071
• Yan, C.R., Mei, X.R., Ju, H. & Chi, J. (2006). Effects of fertilization on the growth, development and water use efficiency of spring millet. Chinese Journal of Eco-Agriculture, 14(1), 142-144.
• Yang, X., Lu, M., Wang, Y., Wang, Y., Liu, Z. & Chen, S. (2021). Response mechanism of plants to drought stress. Horticulturae, 7(3), 50. https://doi.org/10.3390/horticulturae7030050
• Yilmaz, A., Güler, E., Soydemir, H.E., Demirel, S., Mollahaliloğlu, S., Çiftçi, V. & Karadeniz, T. (2021). Miracle Plant: Black Chokeberry (Aronia melanocarpa). MAS Journal of Applied Sciences, 6(1), 83-94. https://doi.org/10.52520/masjaps.20
• Zayova, E., Philipov, P., Nedev, T. & Stoeva, D. (2017). Response of in vitro cultivated eggplant (Solanum melongena L.) to salt and drought stress. AgroLife Scientific Journal, 6(1).
• Zhang, Y., Zhao, Y., Liu, X., Chen, X., Ding, C., Dong, L., ... & Xiao, F. (2021). Chokeberry (Aronia melanocarpa) as a new functional food relationship with health: An overview. Journal of Future Foods, 1(2), 168-178. https://doi.org/10.1016/j.jfutfo.2022.01.006
• Zhong, Y. P., Li, Z., Bai, D. F., Qi, X. J., Chen, J. Y., Wei, C. G., ... & Fang, J. B. (2018). In vitro variation of drought tolerance in five Actinidia species. Journal of the American Society for Horticultural Science, 143(3), 226-234. https://doi.org/10.21273/JASHS04399-18
• Zokaee-Khosroshahi, M., Esna-Ashari, M., Ershadi, A. & Imani, A. (2014). Morphological changes in response to drought stress in cultivated and wild almond species. International Journal of Horticultural Science and Technology, 1(1), 79-92. https://doi.org/10.22059/ijhst.2014.50520