Research Article
BibTex RIS Cite

Effects of harvest time and plant part on essential oils, phenolics, and antioxidant activity in Lippia citriodora

Year 2024, Volume: 8 Issue: 4, 986 - 993, 28.12.2024
https://doi.org/10.31015/jaefs.2024.4.28

Abstract

This study investigates the effects of harvest time and plant parts on the concentrations of essential oils, phenolic content, flavonoids, and antioxidant activities (ABTS and DPPH) in the Lippia citriodora plant to determine the best outcome. The experiment consists of four different harvest times during flowering period and two plant parts (upper and lower). Harvest times were scheduled at weekly intervals.Significant variations were observed during experiment, Essential oil (EO) content reached peak value at the first harvest in upper parts of plants (L1U: 1.18%) and lowest value in lower parts by the fourth harvest (L4L: 0.25%). The highest phenolic content determined at the first harvest (L1: 44.04 mg GAE/g DW), while flavonoid levels reached peak value at the fourth harvest (L4: 314.07 mg rutin/g DW). Antioxidant activities, measured by ABTS and DPPH assays, were significantly greater in lower plant parts. Partial Least Squares Discriminant Analysis (PLS-DA) and Principal Component Analysis Discriminant Analysis (PCA-DA) confirmed clear distinctions between the upper and lower parts of the plant regarding bioactive compounds concentrations. The findings shows the importance of targeted harvest timing and plant parts in optimizing bioactive compound in Lippia citriodora, with implications for getting better benefits from plant.

References

  • Aghdam, A.R., Badi, H.N., Abdossi, V., Hajiaghaee, R., & Hosseini, S.E. (2019). Changes in essential oil content and composition of lemon verbena (Lippia citriodora Kunth.) under various drying conditions. Jundishapur Journal of Natural Pharmaceutical Products, 14(4). https://doi.org/10.5812/jjnpp.66265
  • Argyropoulou, C., Akoumianaki-Ioannidou, A., Christodoulakis, N.S., & Fasseas, C. (2010). Leaf anatomy and histochemistry of Lippia citriodora (Verbenaceae). Australian Journal of Botany, 58(5), 398. https://doi.org/10.1071/bt10072
  • Carrera-Quintanar, L., Funes, L., Viudes, E.B., Tur, J.A., Micol, V., Roche, E., & Pons, A. (2010). Antioxidant effect of lemon verbena extracts in lymphocytes of university students performing aerobic training program. Scandinavian Journal of Medicine & Science in Sports, 22(4), 454-461. https://doi.org/10.1111/j.1600-0838.2010.01244.x
  • Chang, C.H., Lin, H.Y., Chang, C.Y., & Liu, Y.C. (2006). Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes. Journal of Food Engineering, 77, 478-485.
  • Ebadi, M., Sefidkon, F., Azizi, M., & Ahmadi, N. (2016). Packaging methods and storage duration affect essential oil content and composition of lemon verbena (Lippia citriodora Kunth.). Food Science & Nutrition, 5(3), 588-595. https://doi.org/10.1002/fsn3.434
  • Farahmandfar, R., Asnaashari, M., Pourshayegan, M., Maghsoudi, S., & Moniri, H. (2018). Evaluation of antioxidant properties of lemon verbena (Lippia citriodora) essential oil and its capacity in sunflower oil stabilization during storage time. Food Science & Nutrition, 6(4), 983-990. https://doi.org/10.1002/fsn3.637
  • Feduraev, P., Chupakhina, G., Maslennikov, P., Tacenko, N., & Skrypnik, L. (2019). Variation in phenolic compounds content and antioxidant activity of different plant organs from Rumex crispus L. and Rumex obtusifolius L. at different growth stages. Antioxidants, 8(7), 237.
  • Figueiredo, A.C., Barroso, J.G., Pedro, L.G., & Scheffer, J.J.C. (2008). Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal, 23, 213-226.
  • Gadow, A., Joubert, E., & Hansmann, C.F. (1997). Comparison of the antioxidant activity of rooibos tea (Aspalathus linearis) with green oolong and black tea. Food Chemistry, 60, 73-77.
  • Kamal, G.M., Anwar, F., Hussain, A.I., Sarri, N., & Ashraf, M.Y. (2011). Yield and chemical composition of citrus essential oils as affected by drying pretreatment of peels. International Food Research Journal, 18, 1275-1282.
  • Kara, S.M., & Acikgoz, M.A. (2018). Morphogenetic, ontogenetic and diurnal variability in antioxidant activity, total phenol and flavonoids of Foeniculum vulgare Miller var. vulgare extracts. Yuzuncu Yil University Journal of Agricultural Science, 28, 96-101.
  • Karık, Ü., Çınar, O., Tunçtürk, M., & Şekeroğlu, N. (2019). Morphological and diurnal variability of essential oil in lemon verbena (Lippia citriodora HBK). ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, 29(2), 114-120.
  • Kaskoos, R.A. (2019). Essential oil analysis by GC-MS and analgesic activity of Lippia citriodora and Citrus limon. Journal of Essential Oil-Bearing Plants, 22(1), 273-281.
  • Lenoir, L., Rossary, A., Joubert-Zakeyh, J., Vergnaud-Gauduchon, J., Farges, M., Fraisse, D., & Felgines, C. (2011). Lemon verbena infusion consumption attenuates oxidative stress in dextran sulfate sodium-induced colitis in the rat. Digestive Diseases and Sciences, 56(12), 3534-3545. https://doi.org/10.1007/s10620-011-1784-x
  • Maisuthisakul, P., Suttajit, M., & Pongsawatmanit, R. (2007). Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chemistry, 100, 1409-1418.
  • Malayeri, S. H., Hikosaka, S., & Goto, E. (2010). Effects of light period and light intensity on essential oil composition of Japanese mint grown in a closed production system. Environmental Control in Biology, 48(3), 141-149.
  • Mustafa, N.E.M. (2015). Citrus essential oils: Current and prospective uses in the food industry. Recent Patents on Food, Nutrition & Agriculture, 7, 115-127.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231-1237.
  • Shi, T., Su, Y., Lan, Y., Duan, C., & Yu, K. (2024). The molecular basis of flavonoid biosynthesis response to water, light, and temperature in grape berries. Frontiers in Plant Science, 15, 1441893. https://doi.org/10.3389/fpls.2024.1441893
  • Skerget, M., Kotnik, P., Hadolin, M., Hraš, A. R., Simonič, M., & Knez, Ž. (2005). Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chemistry, 89(2), 191-198. https://doi.org/10.1016/j.foodchem.2004.02.025
  • Telci, I., Demirtas, I., & Sahin, A. (2009). Variation in plant properties and essential oil composition of sweet fennel (Foeniculum vulgare Mill.) fruits during stages of maturity. Industrial Crops and Products, 30(1), 126-130.
  • Toncer, O., Karaman, S., Kamci, G., & Erdem, E. (2022). Yield and essential oil composition of Lippia citriodora HBK leaves and flowers in semi-arid conditions. Journal of Essential Oil Bearing Plants, 25(1), 9-19. https://doi.org/10.1080/0972060X.2022.2042399
  • Verma, N., & Shukla, S. (2015). Impact of various factors responsible for fluctuation in plant secondary metabolites. Journal of Applied Research on Medicinal and Aromatic Plants, 2(4), 105-110.
  • Wang, D., Yang, T., Li, Y., Deng, F., Dong, S., Li, W., & Zou, L. (2022). Light intensity—A key factor affecting flavonoid content and expression of key enzyme genes of flavonoid synthesis in tartary buckwheat. Plants, 11(16), 2165.
  • Zhao, X., Yan, Y., Zhou, W., Feng, R., Shuai, Y., Yang, L., … & Qin, W. (2022). Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of cinnamomum longepaniculatum. BMC Plant Biology, 22(1). https://doi.org/10.1186/s12870-022-03637-2.
  • Zoratti, L., Karppinen, K., Luengo Escobar, A., Häggman, H., & Jaakola, L. (2014). Light-controlled flavonoid biosynthesis in fruits. Frontiers in Plant Science, 5, 534. https://doi.org/10.3389/fpls.2014.00534
Year 2024, Volume: 8 Issue: 4, 986 - 993, 28.12.2024
https://doi.org/10.31015/jaefs.2024.4.28

Abstract

References

  • Aghdam, A.R., Badi, H.N., Abdossi, V., Hajiaghaee, R., & Hosseini, S.E. (2019). Changes in essential oil content and composition of lemon verbena (Lippia citriodora Kunth.) under various drying conditions. Jundishapur Journal of Natural Pharmaceutical Products, 14(4). https://doi.org/10.5812/jjnpp.66265
  • Argyropoulou, C., Akoumianaki-Ioannidou, A., Christodoulakis, N.S., & Fasseas, C. (2010). Leaf anatomy and histochemistry of Lippia citriodora (Verbenaceae). Australian Journal of Botany, 58(5), 398. https://doi.org/10.1071/bt10072
  • Carrera-Quintanar, L., Funes, L., Viudes, E.B., Tur, J.A., Micol, V., Roche, E., & Pons, A. (2010). Antioxidant effect of lemon verbena extracts in lymphocytes of university students performing aerobic training program. Scandinavian Journal of Medicine & Science in Sports, 22(4), 454-461. https://doi.org/10.1111/j.1600-0838.2010.01244.x
  • Chang, C.H., Lin, H.Y., Chang, C.Y., & Liu, Y.C. (2006). Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes. Journal of Food Engineering, 77, 478-485.
  • Ebadi, M., Sefidkon, F., Azizi, M., & Ahmadi, N. (2016). Packaging methods and storage duration affect essential oil content and composition of lemon verbena (Lippia citriodora Kunth.). Food Science & Nutrition, 5(3), 588-595. https://doi.org/10.1002/fsn3.434
  • Farahmandfar, R., Asnaashari, M., Pourshayegan, M., Maghsoudi, S., & Moniri, H. (2018). Evaluation of antioxidant properties of lemon verbena (Lippia citriodora) essential oil and its capacity in sunflower oil stabilization during storage time. Food Science & Nutrition, 6(4), 983-990. https://doi.org/10.1002/fsn3.637
  • Feduraev, P., Chupakhina, G., Maslennikov, P., Tacenko, N., & Skrypnik, L. (2019). Variation in phenolic compounds content and antioxidant activity of different plant organs from Rumex crispus L. and Rumex obtusifolius L. at different growth stages. Antioxidants, 8(7), 237.
  • Figueiredo, A.C., Barroso, J.G., Pedro, L.G., & Scheffer, J.J.C. (2008). Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal, 23, 213-226.
  • Gadow, A., Joubert, E., & Hansmann, C.F. (1997). Comparison of the antioxidant activity of rooibos tea (Aspalathus linearis) with green oolong and black tea. Food Chemistry, 60, 73-77.
  • Kamal, G.M., Anwar, F., Hussain, A.I., Sarri, N., & Ashraf, M.Y. (2011). Yield and chemical composition of citrus essential oils as affected by drying pretreatment of peels. International Food Research Journal, 18, 1275-1282.
  • Kara, S.M., & Acikgoz, M.A. (2018). Morphogenetic, ontogenetic and diurnal variability in antioxidant activity, total phenol and flavonoids of Foeniculum vulgare Miller var. vulgare extracts. Yuzuncu Yil University Journal of Agricultural Science, 28, 96-101.
  • Karık, Ü., Çınar, O., Tunçtürk, M., & Şekeroğlu, N. (2019). Morphological and diurnal variability of essential oil in lemon verbena (Lippia citriodora HBK). ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, 29(2), 114-120.
  • Kaskoos, R.A. (2019). Essential oil analysis by GC-MS and analgesic activity of Lippia citriodora and Citrus limon. Journal of Essential Oil-Bearing Plants, 22(1), 273-281.
  • Lenoir, L., Rossary, A., Joubert-Zakeyh, J., Vergnaud-Gauduchon, J., Farges, M., Fraisse, D., & Felgines, C. (2011). Lemon verbena infusion consumption attenuates oxidative stress in dextran sulfate sodium-induced colitis in the rat. Digestive Diseases and Sciences, 56(12), 3534-3545. https://doi.org/10.1007/s10620-011-1784-x
  • Maisuthisakul, P., Suttajit, M., & Pongsawatmanit, R. (2007). Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chemistry, 100, 1409-1418.
  • Malayeri, S. H., Hikosaka, S., & Goto, E. (2010). Effects of light period and light intensity on essential oil composition of Japanese mint grown in a closed production system. Environmental Control in Biology, 48(3), 141-149.
  • Mustafa, N.E.M. (2015). Citrus essential oils: Current and prospective uses in the food industry. Recent Patents on Food, Nutrition & Agriculture, 7, 115-127.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231-1237.
  • Shi, T., Su, Y., Lan, Y., Duan, C., & Yu, K. (2024). The molecular basis of flavonoid biosynthesis response to water, light, and temperature in grape berries. Frontiers in Plant Science, 15, 1441893. https://doi.org/10.3389/fpls.2024.1441893
  • Skerget, M., Kotnik, P., Hadolin, M., Hraš, A. R., Simonič, M., & Knez, Ž. (2005). Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chemistry, 89(2), 191-198. https://doi.org/10.1016/j.foodchem.2004.02.025
  • Telci, I., Demirtas, I., & Sahin, A. (2009). Variation in plant properties and essential oil composition of sweet fennel (Foeniculum vulgare Mill.) fruits during stages of maturity. Industrial Crops and Products, 30(1), 126-130.
  • Toncer, O., Karaman, S., Kamci, G., & Erdem, E. (2022). Yield and essential oil composition of Lippia citriodora HBK leaves and flowers in semi-arid conditions. Journal of Essential Oil Bearing Plants, 25(1), 9-19. https://doi.org/10.1080/0972060X.2022.2042399
  • Verma, N., & Shukla, S. (2015). Impact of various factors responsible for fluctuation in plant secondary metabolites. Journal of Applied Research on Medicinal and Aromatic Plants, 2(4), 105-110.
  • Wang, D., Yang, T., Li, Y., Deng, F., Dong, S., Li, W., & Zou, L. (2022). Light intensity—A key factor affecting flavonoid content and expression of key enzyme genes of flavonoid synthesis in tartary buckwheat. Plants, 11(16), 2165.
  • Zhao, X., Yan, Y., Zhou, W., Feng, R., Shuai, Y., Yang, L., … & Qin, W. (2022). Transcriptome and metabolome reveal the accumulation of secondary metabolites in different varieties of cinnamomum longepaniculatum. BMC Plant Biology, 22(1). https://doi.org/10.1186/s12870-022-03637-2.
  • Zoratti, L., Karppinen, K., Luengo Escobar, A., Häggman, H., & Jaakola, L. (2014). Light-controlled flavonoid biosynthesis in fruits. Frontiers in Plant Science, 5, 534. https://doi.org/10.3389/fpls.2014.00534
There are 26 citations in total.

Details

Primary Language English
Subjects Agronomy
Journal Section Research Articles
Authors

Uğur Tan 0000-0002-9592-2790

Hatice Kübra Gören 0000-0001-7654-1450

Early Pub Date December 27, 2024
Publication Date December 28, 2024
Submission Date November 4, 2024
Acceptance Date December 27, 2024
Published in Issue Year 2024 Volume: 8 Issue: 4

Cite

APA Tan, U., & Gören, H. K. (2024). Effects of harvest time and plant part on essential oils, phenolics, and antioxidant activity in Lippia citriodora. International Journal of Agriculture Environment and Food Sciences, 8(4), 986-993. https://doi.org/10.31015/jaefs.2024.4.28


The International Journal of Agriculture, Environment and Food Sciences content is licensed under a Creative Commons Attribution-NonCommercial (CC BY-NC) 4.0 International License which permits third parties to share and adapt the content for non-commercial purposes by giving the appropriate credit to the original work. Authors retain the copyright of their published work in the International Journal of Agriculture, Environment and Food Sciences. 

Web:  dergipark.org.tr/jaefs  E-mail: editor@jaefs.com WhatsApp: +90 850 309 59 27