Farklı Saklama Koşulları ve İnfüzyon Sürelerinin Kuşburnu Meyvelerinin Antioksidan ve Antimikrobiyal Aktiviteleri ile Toplam Fenolik İçeriği Üzerine Etkisi

Yıl 2024, Cilt: 9 Sayı: 4, 711 - 720

Münevver Darak , Kağan Kılınç , Zeynep Akar , Azer Özad Düzgün , Tuba Acet , Mustafa Nuri Ural , Cemalettin Baltacı , Osman Akmeşe

https://doi.org/10.35229/jaes.1567422

Öz

Rosa canina L. (Kuşburnu) güçlü antioksidan özelliklere sahip tıbbi bir bitkidir. Kuşburnunun içeriği ve buna bağlı olarak antioksidan özelliği meyvelerin kullanım şekline, depolama, infüzyon, dondurma ve kurutma yöntemlerine göre değişebilmektedir. Çalışmamızda, sözkonusu koşullar altında kuşburnu infüzyon ürünleri ve ekstraktlarında antioksidan, antimikrobiyal aktivitelerdeki değişimlerin belirlenmesi amaçlandı. Taze, dondurulmuş, kurutulmuş ve +40C'de depolanmış meyveler 90 0C’de demlenmesiyle demleme grupları oluşturuldu. Demleme sırasında 30, 60, 90 ve 120. 300 ve 360. dakikalarda örnekler alınarak ve antioksidan aktivite (DPPH, FRAP ve ABTS yöntemleri ile), toplam fenolik (TPC), toplam flavonoid içerikleri (TFC) ve antimikrobiyal aktiviteleri analiz edildi. Ayrıca 3 farklı ekstraktta HPLC yöntemi ile fenolik bileşik içeriği araştırıldı. Kurutulmuş kuşburnu (DR) demleme örneklerinin tüm gruplara kıyasla en yüksek antioksidan aktiviteye, TPC ve TFC değerlerine sahip olduğu bulundu (p değeri 0.000 ile 0.05 arasında değişmekteydi). Saklama ve dondurma sürelerine göre parametrelerde anlamlı azalmalar tespit edildi. Kurutulmuş ve 40C'de saklanmış kuşburnu (DR+40C) ve kurutulmuş kuşburnu (DR) meyvelerinin sulu ve etanolik ekstraktlarında en yüksek konsantrasyonu epikateşin olan toplam 8 fenolik bileşik tespit elde edildi. Antimikrobiyal analiz için toplam 7 farklı Gram (+) ve Gram (-) bakteriyel suş kullanıldı. Ekstraktlarda antimikrobiyal aktivite gözlenirken, infüzyon ürünlerinde herhangi bir aktivite görülmedi. Saklama koşullarına göre değişmekle beraber antioksidan aktivite değerlerinin 360 dakika demleme süresi boyunca arttığı tespit edildi.

Anahtar Kelimeler

Antimikrobiyal, Antioksidan, Demleme, Kuşburnu, Saklama

Effect of Different Storage Conditions and Infusion Times on Antioxidant and Antimicrobial Activites and Total Phenolic Content of Rosehip Fruits

Öz

Rosa canina L. (Rosehip) is a medicinal plant with strong antioxidant properties.The content of rosehip and accordingly its antioxidant capability may vary according to the way the fruits are used, storage, infusion, freezing and drying methods. In study, it was aimed to determine the antioxidant, antimicrobial activities in rosehip infusion products and extracts under these conditions. Fresh, frozen, dried and +40C stored fruits were infused at 90 degrees and infusion groups were formed. During infusion, samples were taken at 30, 60, 90, 120, 300 and 360th minutes and antioxidant activity (DPPH, FRAP and ABTS methods), total phenolic and total flavonoid contents (TPC and TFC), and antimicrobial activity were analyzed. In addition, phenolic compound content was investigated in 3 different extracts by HPLC method. Dried rosehip (DR) infusing samples were found to have the highest antioxidant activity, TPC and TFC compared to all groups (p-value ranged between 0.000 and 0.05).). Significant decreases were detected in the parameters according to storage and freezing times. A total of 8 phenolic compounds, the highest concentration of which was epicatechin, were detected in aqueous and ethanolic extracts of dried and stored at 40C rosehip (DR+40C) and dried rosehip (DR) fruits. For antimicrobial analysis, Total 7 different Gram (+) and (Gram (-) bacterial strains, were used. While antimicrobial activity was observed in the extracts, no activity was observed in the infusion products. It was determined that antioxidant activity increased during 360 minute infusion time, although it varied according to storage conditions.

Anahtar Kelimeler

Antimicrobial, Antioxidant, Infusion, Storage, Rosehip

Kaynakça

• Acet, T. & Ozcan, K. (2018). Aslanpençesi (Alchemilla ellenbergiana) ekstrelerinin antioksidan ve antimikrobiyal özelliklerinin belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 8(1), 113-121 DOI: 10.17714/gumusfenbil.308430
• Akar, Z., Orhan, E. & Durmuş, S. (2021). Effects of cold and hot infusions on antioxidant activities of rosehip tea bag. Turkish Journal of Agriculture - Food Science and Technology, 9(8), 1455-1459. DOI: 10.24925/turjaf.v9i8.1455-1459.4276
• Akay, Ş. & Sefaoğlu, M. (2021). Production and characterization of zinc oxide nanoparticles in microsystems via green synthesis. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 11(2), 315-324. DOI: 10.17714/gumusfenbil.819717
• Banerjee, S. & Chatterjee, J. (2015). Efficient extraction strategies of tea (Camellia sinensis) biomolecules. Journal of Food Science and Technology, 52(6), 3158-3168. DOI: 10.1007/s13197-014-1487-3
• Bernatoniene, J. & Kopustinskiene, D. M. (2018). The role of catechins in cellular responses to oxidative stress. Molecule, 23(4), 965. DOI: 10.3390/molecules23040965
• Beara, I.N., Lesjak, M. M., Jovin, E.D., Balog, K.J., Anackov, G. T., Orcić, D.Z. & Mimica-Dukić, N.M. (2009). Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. Journal of agricultural and food chemistry, 57(19), 9268-9273. DOI: 10.1021/jf902205m
• Benzie, I.F. & Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical Biochemistry, 239(1), 70-76. DOI: 10.1006/abio.1996.0292
• Butkevičiūtė, A., Urbštaitė, R., Liaudanskas, M., Obelevičius, K. & Janulis, V. (2022). Phenolic content and antioxidant activity in fruit of the genus Rosa L. Antioxidants (Basel, Switzerland), 11(5), 912 DOI: 10.3390/antiox11050912
• Cabrera, C., Artacho, R. & Giménez, R. (2006). Beneficial effects of green tea--a review. Journal of the American College of Nutrition, 25(2), 79-99. DOI: 10.1080/07315724.2006.10719518
• Chandrasekara, A., & Shahidi, F. (2018). Herbal beverages: Bioactive compounds and their role in disease risk reduction - A review. Journal of Traditional and Complementary Medicine, 8(4), 451-458. DOI: 10.1016/j.jtcme.2017.08.006
• Choi SH. 2009. Essential oil components in herb teas (rose and rosehip). Journal of Life Science, 19(9), 1333- 1336. DOI: 10.5352/JLS.2009.19.9.1333
• Darak, M. (2022). Geleneksel tıpta kullanılan kuşburnu (Rosa canina L.) meyvelerinin farklı saklama koşulları ve demleme sürelerinde antioksidan antimikrobiyal aktivitelerinin ve fenolik içeriğinin incelenmesi. Gümüşhane Üniversitesi, Lisansüstü Eğitim Enstitüsü. Gümüşhane, Türkiye, 55s.
• Faydaoğlu, E. & Sürücüoğlu, M.S. (2011). Geçmisten günümüze tıbbi ve aromatik bitkilerin kullanılması ve ekonomik önemi, Kastamonu Üniversitesi Orman Fakültesi Dergisi, 11(1), 52-67.
• Giannakourou, M.C. & Taoukis, P.S. (2021). Effect of alternative preservation steps and storage on vitamin C stability in fruit and vegetable products: Critical review and kinetic modelling approaches. Foods (Basel, Switzerland), 10(11), 2630. DOI: 10.3390/foods10112630
• Göktaş, Ö. & Gıdık, B. (2019). Tıbbi ve Aromatik Bitkilerin Kullanım Alanları. Bayburt Üniversitesi Fen Bilimleri Dergisi, 2(1), 145-151.
• Gruszecki, R., Mysiak, B. & Walasek-Janusz, M. (2024). Effect drying temparature on the bioactive compounds contents of Rose hips (Rosa canina L.) Acta Sci. Pol. Hortorum Cultus, 23(3), 57-66. DOI: 10.24326/asphc.2024.5348
• Hacioglu, M., Dosler, S., Birteksoz Tan, A.S. & Otuk, G. (2017). Antimicrobial activities of widely consumed herbal teas, alone or in combination with antibiotics: an in vitro study. PeerJ, 5,e3467. DOI: 10.7717/peerj.3467
• İlyasoğlu, H. & Arpa, T.E. (2017). Effect of brewing conditions on antioxidant properties of rosehip tea beverage: study by response surface methodology. Journal of food science and technology, 54(11), 3737-3743. DOI: 10.1007/s13197-017-2794-2
• Kadiroğlu, P. & Dıblan, S. (2017). Comparison of bioactive and antimicrobial properties of Black and Green Teas. Çukurova Tarım Ve Gıda Bilimleri Dergisi, 32(1), 13-18.
• Kilinc, K., Demir, S., Turan, I., Mentese, A., Orem, A., Sonmez, M. & Aliyazicioglu, Y. (2020). Rosa canina extract has antiproliferative and proapoptotic effects on human lung and prostate cancer cells. Nutr Cancer. 72(2), 273-282. DOI: 10.1080/01635581.2019.1625936
• Kocaman, E.M. & Sormaz, Ü. (2023). Fonksiyonel bir besin olan kuşburnu meyvesinin Türkiye’de tüketimi. Safran Kültür ve Turizm Araştırmaları Dergisi, 6(3), 435-453.
• Mieczan, A.W. & Wójcik, E.B. (2024). The Effect of brewing time on the antioxidant activity of tea Infusions. Applied Sciences, 14(5), 1-11. DOI: 10.3390/app14052014
• Miljković, V.M., Nikolić, L., Mrmošanin, J., Gajić, I., Mihajilov-Krstev, T., Zvezdanović, J. & Miljković, M. (2024). Chemical profile and antioxidant and antimicrobial activity of Rosa canina L. dried fruit commercially available in Serbia. International journal of molecular sciences, 25(5), 2518. DOI: 10.3390/ijms25052518
• Moşanu, A.G., Cojocari, D., Balan, G., & Sturza, R. (2018). Antimicrobial activity of rose hip and hawthorn powders on pathogenic bacteria. Journal of Engineering Science, 15(4),100-107. DOI: 10.5281/zenodo.2576764
• Ghendov-Moșanu, A., Cojocari, D., Balan, G. & Sturza, R. (2018). Antimicrobial activity of rose hip and hawthorn powders on pathogenic bacteria. Journal of Engineering Science, 15(4), 100-107. DOI: 10.5281/zenodo.2576764
• Nadpal, J.D., Lesjak, M.M., Šibul, F.S., Anačkov, G.T., Četojević-Simin, D.D., Mimica-Dukić, N.M. & Beara, I.N. (2016). Comparative study of biological activities and phytochemical composition of two rose hips and their preserves: Rosa canina L. and Rosa arvensis huds. Food Chem., 1(192), 907-914.
• Negrean, O.R., Farcas, A.C., Nemes, S.A., Cic, D.E. & Socaci, S.A. (2024). Recent advances and insights into the bioactive properties and applications of Rosa canina L. and its by-products. Heliyon, 10(9), e30816. DOI: 10.1016/j.heliyon.2024.e3081
• Oz, M., Baltacı, C. & Deniz, İ. (2018). Gümüşhane yöresi kuşburnu (Rosa canina L.) ve siyah kuşburnu (Rosa pimpinellifolia L.) meyvelerinin c vitamini ve şeker analizleri. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2, 284-292. DOI: 10.17714/gumusfenbil.327635
• Paje, L.A., Choi, J., Lee, H.D., Kim, J., Yu, A.R., Bae, M.J., Geraldino, P.J.L. & Lee, S. (2022). Phenolic acids and flavonoids from Salvia plebeia and HPLC-UV profiling of four Salvia species. Heliyon, 8(3), e, 09046. DOI: 10.1016/j.heliyon.2022.e09046
• Riaz, M., Khalid, R., Afzal, M., Anjum, F., Fatima, H., Zia, S., Rasool, G., Egbuna, C., Mtewa, A.G., Uche, C.Z. & Aslam, M.A. (2023). Phytobioactive compounds as therapeutic agents for human diseases: A review. Food science & nutrition, 11(6), 2500-2529. DOI: 10.1002/fsn3.330
• Roman, I., Stănilă, A. & Stănilă, S. (2013). Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chemistry Central journal, 7(1), 73. DOI: 10.1186/1752-153X-7-73
• Safarov, J. (2020). Drying medicinal plants with the storage of antioxidant properties. International Journal of Advanced Science and Technology, 29(9), 20115- 2119.
• Saklar, S., Ertas, E., Ozdemir, I.S. & Karadeniz, B. (2015). Effects of different brewing conditions on catechin content and sensory acceptance in Turkish green tea infusions. Journal of Food Science and Technology, 52(10), 6639-6646. DOI: 10.1007/s13197-015- 1746-y
• Selahvarzian, A., Alizade, A., Baharvand, P.A., Eldahşan, O.A. & Rasulian, B. (2018). Medicinal properties of Rosa canina L. Herbal Medicines Journal, 3(2), 77-84. DOI: 10.22087/hmj.v3i2.703
• Silva, E.M., Souza, J.N.S., Rogez, H., Rees, J.F. & Y, Larondelle. (2007). Antioxidant activities and polyphenolic contents of fifteen selected plant species from the Amazonian region, Food Chemistry, 101(3), 1012-1018. DOI: 10.1016/j.foodchem.2006.02.055
• Singleton, V. L., Orthofer, R. & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of FolinCiocalteu reagent, Methods in Enzymol. 299, 152- 178. DOI: 10.1016/S0076-6879(99)99017-1
• Soler-Rivas, C., Espín, J.C. & Wichers, H.J., (2000). An easy and fast test to compare total free radical scavenger capacity of foodstuffs.Phytochemical Analysis, 11(5),330-338.
• Tastekin, B. & Çiftci, G. (2023). Antioxidant Capacity and Antibacterial Potential of Rosehip (Rosa Canina) Fruits Grown. Journal of Anatolian Environmental and Animal Sciences, 8(1), 103-109. DOI: 10.35229/jaes.1240877
• Tiring, G., Satar, S. & Özkaya, O. (2021). Sekonder metabolitler. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 35(1), 203-2015
• Toydemir, G., Subasi, B.G., Hall, R. D., Beekwilder, J., Boyacioglu, D. & Capanoglu, E. (2022). Effect of food processing on antioxidants, their bioavailability and potential relevance to human health. Food Chemistry: X, 14, 100334. DOI: 10.1016/j.fochx.2022.100334
• Turan, I., Demir, S., Kilinc, K., Yaman, S.O., Misir, S., Kara, H., Genc, B., Mentese, A., Aliyazicioglu, Y. & Deger, O. (2018). Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomeraseexpression. Journal of pharmaceutical analysis, 8(6), 394-399. DOI: 10.1016/j.jpha.2017.12.005
• Turkben. C., Uylaser, V., İncedayı, B. & Çelikkol, I. (2010). Effects of different maturity periods and processes on nutritional components of rose hip (Rosa canina L.), Journal of Food Agriculture and Environment, 8(1), 26-30.
• Yildirim, R.M., Ozulku, G., Toker, O.S., Baslar, M., Durak, M.Z. & Sagdic, O. (2017), Modeling of bioactive compound content of different tea bags: effect of steeping temperature and time. Journal of Food Processing and Preservation, 41, e12773. DOI: 10.1111/jfpp.12773
• Yilmaz, S.O. & Ercişli, S. (2011). Antibacterial and antioxidant activity of fruits of some rose species from Turkey. Romanian Biotechnological Letters, 16(4), 6407-6411.
• Wenzig, E.M., Widowitz, U., Kunert, O., Chrubasik, S., Bucar, F., Knauder, E. & Bauer, R. (2008). Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations. Phytomedicine : international journal of phytotherapy and phytopharmacology, 15(10), 826-835. DOI: 10.1016/j.phymed.2008.06.012
• Zohora, K.F.T. & Arefin, M.R. (2022). Tea and Tea Product Diversification: A Review. Turkish Journal of Agriculture-Food Science and Technology, 10(12), 2334-2353. DOI: 10.24925/turjaf.v10i12.2334- 2353.5280.