Developing Lucilia sericata flies on natural and artificial substrates containing natural ingredients

Year 2025, Volume: 10 Issue: 2, 143 - 150

Nevra Polat , Salih Mollahaliloğlu , Murat Koç

https://doi.org/10.35229/jaes.1608450

Abstract

Foods with high protein and organic content are effective in the maturation of sperm and eggs of Calliphoridae and mating. In Lucilia sericata fly colony continuity, liver food in the procedure in terms of both chemical properties and protein and moisture content is a suitable food substrate for females to lay their eggs. However, liver or any other animal tissue can cause contamination due to decomposition during larval development of the eggs. Many sterilised artificial diets have been developed for controlled mass rearing. In this study, natural diet liver and liver-agar artificial diets containing natural diet were preferred after spawning. In particular, the effects of these diets on the following characteristics of L sericata were analysed: 1) growth and development time 2) larval stage length and weight 3) pupal and adult length 4) morphological appearance of larvae, pupae and adults. The experiment was conducted in two replicate experiments with six study groups in each. In general, rearing on liver-based media was similar, but growth and development on sterile liver-agar media was more efficient than on liver and no contamination occurred. There was a significant difference (p<0.05) between measurements of first instar larval length, first instar larval weight, second instar larval weight, third instar larval length, third instar larval weight, pupal length and adult length of L. sericata larvae fed on liver and liver-agar. However, there was no significant difference between the second instar larval length measurements of L. sericata larvae fed with liver-agar and liver (p>0.05).

Keywords

Artificial substrate, growth and development, larval feeding, Lucilia sericata

Thanks

We thank Ankara Yıldırım Beyazıt University entomology researchers suggestions on an earlier draft of this manuscript. We also thank laborotory technicians for their helps in laboratory studies.

Lucilia sericata sineklerinin doğal ve doğal maddeler içeren substratlar üzerinde geliştirilmesi

Abstract

Calliphoridae üyelerinin sperm ve yumurtalarının olgunlaşmasıyla çiftleşmesinde protein oranı yüksek, organik içerikli besinler etkilidir. Lucilia sericata sinek koloni sürekliliğinde hem kimyasal özelliği hem de protein ve nem içeriği bakımından prosedürdeki ciğer besini dişilerin yumurtalarını bırakmasında uygun besin substratıdır. Ancak yumurtaların larval gelişiminde ciğer veya herhangi bir hayvansal doku çürümeye bağlı olarak kontaminasyona sebep olabilir. Kontrollü kitlesel yetiştirme için birçok sterilize yapay diyet geliştirilmiştir. Bu çalışmada yumurtlamayı takiben doğal diyet ciğer ile doğal diyet içerikli ciğer-agar yapay diyet tercih edildi. Özellikle bu besin ortamlarında Lucilia sericata’nın şu özellikleri üzerindeki etkileri incelendi: 1) büyüme ve gelişme zamanları 2) larval dönemsel evre boy ve ağırlıkları 3) pupa ve ergin boyları 4) larva, pupa ve ergin morfolojik görünüm. Deney her birinde altışar çalışma grupları ile tekrar teknik bulunan iki deneme ile sürdürüldü. Genel olarak ciğer bazlı besin ortamlarında yetiştirme benzerdi ancak steril ciğer-agar ortamında büyüme ve gelişim ciğerdeki gelişme kıyasla daha etkili idi ve kontaminasyon gelişmedi. Lucilia sericata larvalarından ciğer ve ciğer-agar ile beslenen birinci dönem larva boy, birinci dönem larva ağırlık, ikinci dönem larva ağırlık, üçüncü dönem larva boy, üçüncü dönem larva ağırlık, pupaya geçiş dönemindeki boy ve ergin dönemindeki boy ölçümleri arasında anlamlı fark elde edilmiştir (p<0,05). Ancak ciğer-agar ve ciğer ile beslenen Lucilia sericata larvalarının ikinci dönem larva boy ölçümleri arasında fark yoktur (p>0,05).

Keywords

Büyüme ve gelişme, larva beslenmesi, Lucilia sericata, yapay substrat

References

• Barnes, K.M. & Gennard, D.E. (2013). Rearing bacteria and maggots concurrently: a protocol using Lucilia sericata (Diptera: Calliphoridae) as a model species. Applied Entomology and Zoology, 48, 247- 253.
• Benbow, M.E., Barton, P.S., Ulyshen, M.D., Beasley, J.C., DeVault, T.L., Strickland, M.S., & Pechal, J.L. (2019). Necrobiome framework for bridging decomposition ecology of autotrophically and heterotrophically derived organic matter. Ecological Monographs, 89(1), e01331.
• Benbow, M.E. Pechal, J.L. & Mohr, R.M. (2015). Community and landscape ecology of carrion. Carrion Ecology, Evolution, and Their Applications, 151-186.
• Bomphrey, R.J., Walker, S.M., & Taylor, G.K. (2009). The typical flight performance of blowflies: measuring the normal performance envelope of Calliphora vicina using a novel corner-cube arena. PLoS One, 4(11), e7852.
• Burkepile, D.E., Parker, J.D., Woodson, C.B., Mills, H.J., Kubanek, J., Sobecky, P.A. & Hay, M.E. (2006). Chemically mediated competition between microbes and animals: microbes as consumers in food webs. Ecology, 87(11), 2821-2831.
• Byrd, J.H. & Castner, J.L. (2001). Insects of forensic importance. Forensic Entomology: The Utility of Arthropods in Legal Investigations, 43-79.
• Clark, K., Evans, L. & Wall, R. (2006). Growth rates of the blowfly, Lucilia sericata, on different body tissues. Forensic science international, 156(2-3), 145-149.
• Cotter, S.C., Kruuk, L.E.B. & Wilson, K. (2004). Costs of resistance: genetic correlations and potential trade‐ offs in an insect immune system. Journal of Evolutionary Biology, 17(2), 421-429.
• Çoban, E. (2009). Edirne ili Trakya Üniversitesi Güllapoğlu Yerleşkesi'nde adli entomoloji yönünden önem taşıyan Diptera faunasının leş üzerinden toplanması ve taksonomik yönden incelenmesi.
• Early, M. & Goff, M.L. (1986). Arthropod succession patterns in exposed carrion on the island of O'Ahu, Hawaiian Islands, USA. Journal of Medical Entomology, 23, 520-531.
• El-Moaty, Z.A. & Abd Elmoneim, M.K. (2013). Developmental variation of the blow fly Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) by different substrate tissue types. Journal of Asia- Pacific Entomology, 16(3), 297-300.
• Espra, A. S., Tabugo, S. R. M., Torres, M. A. J., Gorospe, J. G., Manting, M. M. E., & Demayo, C. G. (2015). Describing dimorphism in wing shapes in the blowfly Lucilia sericata Meigen (Diptera: Calliphoridae) using geometric morphometrics. Advances in Environmental Biology, 9(19 S4), 64- 71.
• Faris, A.M., West, W.R., Tomberlin, J.K. & Tarone, A.M. (2020). Field validation of a development data set for Cochliomyia macellaria (Diptera: Calliphoridae): estimating insect age based on development stage. Journal of Medical Entomology, 57(1), 39-49.
• Firoozfar, F., Moosa-Kazemi, H., Baniardalani, M., Abolhassani, M., Khoobdel, M. & Rafinejd, J. (2011). Mass rearing of Lucilia sericata Meigen (Diptera: Calliphoridae). Asian Pacific Journal of Tropical Biomedicine, 1(1), 54-56.
• Gomes, L., Gomes, G., Oliveira, H.G., Sanches, M.R. & Von Zuben, C.J. (2006). Influence of photoperiod on body weight and depth of burrowing in larvae of Chrysomya megacephala (Fabricius) (Diptera, Calliphoridae) and implications for forensic entomology. Revista Brasileira de Entomologia, 50, 76-79.
• Greenberg, B. & Kunich, J.C. (2002). Entomology and the law: flies as forensic indicators. Cambridge University Press.
• Gunn, A. (2019). Essential forensic biology. John Wiley & Sons.
• Hassan, A. (2008). Influence of food type on larval growth in Lucilia sericata, London: School of Biosciences, University of Westminster; 1-26 p.
• Komo, L. & Charabidze, D. (2021). Balance between larval and pupal development time in carrion blowflies. Journal of Insect Physiology, 133, 104292.
• Mohr, R.M. (2012). Female blow fly (Diptera: Calliphoridae) arrival patterns and consequences for larval development on ephemeral resources. Texas A&M University.
• Pallant, J.F. & Tennant, A. (2007). An introduction to the Rasch measurement model: an example using the Hospital Anxiety and Depression Scale (HADS). British Journal of Clinical Psychology, 46(1), 1-18.
• Shaaya, E. & Levenbook, L. (1982). The effects of starvation and 20-hydroxy-ecdysone on feeding and pupariation of early 3rd-instar Calliphora vicina larvae. Journal of İnsect Physiology, 28(8), 683-688.
• Sherman, R.A. (1995). What physicians should know about Africanized honeybees. Western Journal of Medicine, 163(6), 541.
• Sherman, R.A. & My-Tien Tran, J.M.A. (1995). A simple, sterile food source for rearing the larvae of Lucilia sericata (Diptera: Calliphoridae), Medical and Veterinary Entomology 9, 393-398.
• Tabachnick, B.G & Fidell, L.S. (2013). Using Multivariate Statistics (6th ed.). Boston: Pearson Publishing.
• Tachibana, S.I. & Numata, H. (2001). An artificial diet for blow fly larvae, Lucilia sericata (Meigen) (Diptera: Calliphoridae). Applied Entomology and Zoology, 36(4), 521-523.
• Tarone, A.M. & Foran, D.R. (2006). Components of developmental plasticity in a Michigan population of Lucilia sericata (Diptera: Calliphoridae). Journal of Medical Entomology, 43(5), 1023-1033.
• Wollina, U., Karte, K., Herold, C. & Looks, A. (2000). Biosurgery in wound healing–the renaissance of maggot therapy. Journal of the European Academy of Dermatology and Venereology, 14(4), 285-289.