Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves

Year 2025, Volume: 8 Issue: 2, 128 - 142, 31.12.2025

Yağmur Demirel Özbek , Özlem Saral , Barış Karslı

https://doi.org/10.35206/jan.1657082

Abstract

Trachystemon orientalis L. is a plant belonging to the Boraginaceae family and growing in the Black Sea region. In this study, antioxidant and antimicrobial activities of T. orientalis (L.) were determined by different extraction preparation methods with water. Total phenolic content (TPC), DPPH and FRAP values, phenolic content and antimicrobial activity of the extracts were analyzed by HPLC extraction method that showed the highest antioxidant activity and total phenolic content was the T. orientalis (L.) plant extract boiled for 30 minutes. According to the antimicrobial activity results, the most sensitive bacteria to the extracts was S. enterica. The highest antimicrobial activity was found in boiling and resting extraction. According to HPLC analysis results, campferol was detected in T. orientalis (L.) leaves in boiling and resting extraction. In conclusion, the boiling extraction of T. orientalis (L.) revealed good antioxidant and antimicrobial activity.

Keywords

Antioxidant activity , Antimicrobial activity , Phenolic compounds , T. orientalis L

Trachystemon orientalis (L.) Yapraklarının Farklı Sulu Ekstraksiyon Yöntemlerinin Antioksidan Aktivite ve Antimikrobiyal Aktivitesinin Belirlenmesi

Abstract

Trachystemon orientalis L., Boraginaceae familyasına ait ve Karadeniz bölgesinde yetişen bir bitkidir. Bu çalışmada, T. orientalis (L.)'in antioksidan ve antimikrobiyal aktiviteleri su ile farklı ekstraksiyon hazırlama yöntemleri ile belirlendi. Ekstraktların toplam fenolik içeriği (TPC), DPPH ve FRAP değerleri, antimikrobiyal aktivitesi ve fenolik içeriği HPLC ile analiz edildi. En yüksek antioksidan aktivite ve toplam fenolik içeriği gösteren ekstraksiyon yöntemi 30 dakika kaynatılan T. orientalis (L.) bitki ekstraktı olmuştur. Antimikrobiyal aktivite sonuçlarına göre, ekstraktlara karşı en hassas bakteri S. enterica’dır. En yüksek antimikrobiyal aktivite kaynatma ve dinlendirme ekstraksiyonunda bulunmuştur. HPLC analiz sonuçlarına göre, T. orientalis (L.) yapraklarında kaynatma ve dinlendirme ekstraksiyonlarında kampferol tespit edilmiştir. Sonuç olarak, T. orientalis (L.)'in kaynatma ekstraksiyonu iyi antioksidan ve antimikrobiyal aktivite göstermiştir.

Keywords

Antioksidan aktivite , Antimikrobiyal aktivite , Fenolik bileşikler , T. orientalis L

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