Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves

Yağmur Demirel Özbek; Özlem Saral; Barış Karslı

doi:10.35206/jan.1657082

EN TR

Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves

Abstract

Trachystemon orientalis L. is a plant belonging to the Boraginaceae family and growing in the Black Sea region. In this study, antioxidant and antimicrobial activities of T. orientalis (L.) were determined by different extraction preparation methods with water. Total phenolic content (TPC), DPPH and FRAP values, phenolic content and antimicrobial activity of the extracts were analyzed by HPLC extraction method that showed the highest antioxidant activity and total phenolic content was the T. orientalis (L.) plant extract boiled for 30 minutes. According to the antimicrobial activity results, the most sensitive bacteria to the extracts was S. enterica. The highest antimicrobial activity was found in boiling and resting extraction. According to HPLC analysis results, campferol was detected in T. orientalis (L.) leaves in boiling and resting extraction. In conclusion, the boiling extraction of T. orientalis (L.) revealed good antioxidant and antimicrobial activity.

Keywords

Antioxidant activity , Antimicrobial activity , Phenolic compounds , T. orientalis L

Trachystemon orientalis (L.) Yapraklarının Farklı Sulu Ekstraksiyon Yöntemlerinin Antioksidan Aktivite ve Antimikrobiyal Aktivitesinin Belirlenmesi

Öz

Trachystemon orientalis L., Boraginaceae familyasına ait ve Karadeniz bölgesinde yetişen bir bitkidir. Bu çalışmada, T. orientalis (L.)'in antioksidan ve antimikrobiyal aktiviteleri su ile farklı ekstraksiyon hazırlama yöntemleri ile belirlendi. Ekstraktların toplam fenolik içeriği (TPC), DPPH ve FRAP değerleri, antimikrobiyal aktivitesi ve fenolik içeriği HPLC ile analiz edildi. En yüksek antioksidan aktivite ve toplam fenolik içeriği gösteren ekstraksiyon yöntemi 30 dakika kaynatılan T. orientalis (L.) bitki ekstraktı olmuştur. Antimikrobiyal aktivite sonuçlarına göre, ekstraktlara karşı en hassas bakteri S. enterica’dır. En yüksek antimikrobiyal aktivite kaynatma ve dinlendirme ekstraksiyonunda bulunmuştur. HPLC analiz sonuçlarına göre, T. orientalis (L.) yapraklarında kaynatma ve dinlendirme ekstraksiyonlarında kampferol tespit edilmiştir. Sonuç olarak, T. orientalis (L.)'in kaynatma ekstraksiyonu iyi antioksidan ve antimikrobiyal aktivite göstermiştir.

Anahtar Kelimeler

Antioksidan aktivite , Antimikrobiyal aktivite , Fenolik bileşikler , T. orientalis L

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Gıda Mühendisliği

Bölüm

Araştırma Makalesi

Yazarlar

Yağmur Demirel Özbek ^*
0000-0003-3877-3183
Türkiye

Özlem Saral
0000-0002-5283-7000
Türkiye

Barış Karslı
0000-0002-3944-6988
Türkiye

Yayımlanma Tarihi

31 Aralık 2025

Gönderilme Tarihi

13 Mart 2025

Kabul Tarihi

30 Kasım 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 8 Sayı: 2

DOI

https://doi.org/10.35206/jan.1657082

IZ

https://izlik.org/JA57RN73TK

APA

Demirel Özbek, Y., Saral, Ö., & Karslı, B. (2025). Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves. Journal of Apitherapy and Nature, 8(2), 128-142. https://doi.org/10.35206/jan.1657082

AMA

1.Demirel Özbek Y, Saral Ö, Karslı B. Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves. Journal of Apitherapy and Nature. 2025;8(2):128-142. doi:10.35206/jan.1657082

Chicago

Demirel Özbek, Yağmur, Özlem Saral, ve Barış Karslı. 2025. “Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves”. Journal of Apitherapy and Nature 8 (2): 128-42. https://doi.org/10.35206/jan.1657082.

EndNote

Demirel Özbek Y, Saral Ö, Karslı B (01 Aralık 2025) Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves. Journal of Apitherapy and Nature 8 2 128–142.

IEEE

[1]Y. Demirel Özbek, Ö. Saral, ve B. Karslı, “Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves”, Journal of Apitherapy and Nature, c. 8, sy 2, ss. 128–142, Ara. 2025, doi: 10.35206/jan.1657082.

ISNAD

Demirel Özbek, Yağmur - Saral, Özlem - Karslı, Barış. “Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves”. Journal of Apitherapy and Nature 8/2 (01 Aralık 2025): 128-142. https://doi.org/10.35206/jan.1657082.

JAMA

1.Demirel Özbek Y, Saral Ö, Karslı B. Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves. Journal of Apitherapy and Nature. 2025;8:128–142.

MLA

Demirel Özbek, Yağmur, vd. “Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves”. Journal of Apitherapy and Nature, c. 8, sy 2, Aralık 2025, ss. 128-42, doi:10.35206/jan.1657082.

Vancouver

1.Yağmur Demirel Özbek, Özlem Saral, Barış Karslı. Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves. Journal of Apitherapy and Nature. 01 Aralık 2025;8(2):128-42. doi:10.35206/jan.1657082

e-ISSN: 2667-4734 Yayın Modeli: Sürekli Yayın Atıf Dizinleri: TR Dizin

Determination of Antioxidant Activity and Antimicrobial Activity of Different Aqueous Extraction Methods of Trachystemon orientalis (L.) Leaves

Abstract

Keywords

Trachystemon orientalis (L.) Yapraklarının Farklı Sulu Ekstraksiyon Yöntemlerinin Antioksidan Aktivite ve Antimikrobiyal Aktivitesinin Belirlenmesi

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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