Propolis çok sayıda biyolojik aktiviteye sahip olan polifenoller
bakımından zengindir. Günümüzde birçok araştırmacı, geniş biyolojik aktivitesine
atfen propolise odaklanmış ve bu nedenle propolis fonksiyonel bir gıda olarak
nitelendirilmiştir. Biyolojik aktivitenin değerlendirilmesinde in vitro
propolis sindirilebilirliği önemli bir faktördür. Bu çalışma, Türk
propolisinin su, etanol ve monopropilen glikol ekstraktlarının biyoerişilebilirliğinin
in vitro gastrointestinal sindirim ile değerlendirilmesi için tasarlanmıştır.
Toplam polifenol içeriği Folin-Ciocalteu kolorimetrik yöntemi ile araştırılmıştır.
Ekstraktların antioksidan aktiviteleri, demir indirgeyici antioksidan güç
(FRAP) analizi ile belirlenmiştir. Sindirim fraksiyonlarının toplam fenolik
madde içeriği ve antioksidan aktivitelerinde sindirime uğratılmamış
ekstraktlara göre belirgin azalmalar bulunmuştur. Propolis ekstraktlarının
toplam fenolik madde içeriğinde ve antioksidan aktivitelerinde sırası ile
yaklaşık %87 ve %91 kayıp tespit edilmiştir.
Benzie, I. F., & Strain, J. J. (1999). [2] Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. In Methods in Enzymology (Vol. 299, pp. 15-27). Academic Press.
Burdock, G. A. (1998). Review of the biological properties and toxicity of bee propolis (propolis). Food and Chemical toxicology, 36(4), 347-363.
Huang, S., Zhang, C. P., Wang, K., Li, G. Q., & Hu, F. L., 2014. Recent advances in the chemical composition of propolis. Molecules, 19(12), 19610-19632, 2014.
Laskar, R. A., Sk, I., Roy, N., & Begum, N. A. (2010). Antioxidant activity of Indian propolis and its chemical constituents. Food Chemistry, 122(1), 233-237.
McDougall, G. J., Dobson, P., Smith, P., Blake, A., & Stewart, D. (2005). Assessing potential bioavailability of raspberry anthocyanins using an in vitro digestion system. Journal of agricultural and food chemistry, 53(15), 5896-5904.
Ozdal, T., Ceylan, F. D., Eroglu, N., Kaplan, M., Olgun, E. O., & Capanoglu, E. (2019). Investigation of antioxidant capacity, bioaccessibility and LC-MS/MS phenolic profile of Turkish propolis. Food Research International.
Popova, M. P., Graikou, K., Chinou, I., & Bankova, V. S. (2010). GC-MS profiling of diterpene compounds in Mediterranean propolis from Greece. Journal of agricultural and food chemistry, 58(5), 3167-3176.
Ristivojević, P., Dimkić, I., Guzelmeric, E., Trifković, J., Knežević, M., Berić, T., ... & Stanković, S. (2018). Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities. LWT, 95, 367-379.
Sforcin, J. M., & Bankova, V. (2011). Propolis: is there a potential for the development of new drugs?. Journal of ethnopharmacology, 133(2), 253-260.
Sforcin, J. M., Bankova, V., & Kuropatnicki, A. K. (2017). Medical benefits of honeybee products. Evidence-Based Complementary and Alternative Medicine, 2017.
Silva, J. C., Rodrigues, S., Feás, X., & Estevinho, L. M. (2012). Antimicrobial activity, phenolic profile and role in the inflammation of propolis. Food and Chemical Toxicology, 50(5), 1790-1795.
Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology, 299, 152–178.
Sun, C., Wu, Z., Wang, Z., & Zhang, H. (2015). Effect of ethanol/water solvents on phenolic profiles and antioxidant properties of Beijing propolis extracts. Evidence-Based Complementary and Alternative Medicine, 2015.
Torres, A. R., Sandjo, L. P., Friedemann, M. T., Tomazzoli, M. M., Maraschin, M., Mello, C. F., & Santos, A. R. S. (2018). Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees. Brazilian Journal of Medical and Biological Research, 51(6).
Viuda-Martos, M., Ruiz-Navajas, Y., Fernández-López, J., & Pérez-Álvarez, J. A. (2008). Functional properties of honey, propolis, and royal jelly. Journal of food science, 73(9), R117-R124.
Yen, C. H., Chiu, H. F., Wu, C. H., Lu, Y. Y., Han, Y. C., Shen, Y. C., ... & Wang, C. K. (2017). Beneficial efficacy of various propolis extracts and their digestive products by in vitro simulated gastrointestinal digestion. LWT, 84, 281-289.
Yesiltas, B., Capanoglu, E., Firatligil-Durmus, E., Sunay, A. E., Samanci, T., & Boyacioglu, D. (2014). Investigating the in-vitro bioaccessibility of propolis and pollen using a simulated gastrointestinal digestion System. Journal of Apicultural Research, 53(1), 101-108.
Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared with Various Solvents
Propolis is rich in polyphenols with a large number of biological activities.
Many researchers currently focusing on propolis attributing to its broad
spectrum of biological activities and thus considered as a functional food. In
vitro propolis digestibility is an important factor on evaluation of
biological activity. This study was designed to assess bioaccessibility
alterations of water, ethanol, and monopropylene glycol
extracts of Turkish propolis by in vitro gastrointestinal digestion.The total content of
polyphenols was investigated by Folin–Ciocalteu colorimetric method. Antioxidant
activities of extracts was estimated by ferric reducing antioxidant power
(FRAP) assays. Significant decreases were found in the total phenolic and antioxidant
capacities in the digested fractions when compared to the undigested
extracts. Approximately 87 and 91% losses were determined in the total
phenolic content and antioxidant activity of propolis extracts after in vitro post gastric digestion, respectively.
Benzie, I. F., & Strain, J. J. (1999). [2] Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. In Methods in Enzymology (Vol. 299, pp. 15-27). Academic Press.
Burdock, G. A. (1998). Review of the biological properties and toxicity of bee propolis (propolis). Food and Chemical toxicology, 36(4), 347-363.
Huang, S., Zhang, C. P., Wang, K., Li, G. Q., & Hu, F. L., 2014. Recent advances in the chemical composition of propolis. Molecules, 19(12), 19610-19632, 2014.
Laskar, R. A., Sk, I., Roy, N., & Begum, N. A. (2010). Antioxidant activity of Indian propolis and its chemical constituents. Food Chemistry, 122(1), 233-237.
McDougall, G. J., Dobson, P., Smith, P., Blake, A., & Stewart, D. (2005). Assessing potential bioavailability of raspberry anthocyanins using an in vitro digestion system. Journal of agricultural and food chemistry, 53(15), 5896-5904.
Ozdal, T., Ceylan, F. D., Eroglu, N., Kaplan, M., Olgun, E. O., & Capanoglu, E. (2019). Investigation of antioxidant capacity, bioaccessibility and LC-MS/MS phenolic profile of Turkish propolis. Food Research International.
Popova, M. P., Graikou, K., Chinou, I., & Bankova, V. S. (2010). GC-MS profiling of diterpene compounds in Mediterranean propolis from Greece. Journal of agricultural and food chemistry, 58(5), 3167-3176.
Ristivojević, P., Dimkić, I., Guzelmeric, E., Trifković, J., Knežević, M., Berić, T., ... & Stanković, S. (2018). Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities. LWT, 95, 367-379.
Sforcin, J. M., & Bankova, V. (2011). Propolis: is there a potential for the development of new drugs?. Journal of ethnopharmacology, 133(2), 253-260.
Sforcin, J. M., Bankova, V., & Kuropatnicki, A. K. (2017). Medical benefits of honeybee products. Evidence-Based Complementary and Alternative Medicine, 2017.
Silva, J. C., Rodrigues, S., Feás, X., & Estevinho, L. M. (2012). Antimicrobial activity, phenolic profile and role in the inflammation of propolis. Food and Chemical Toxicology, 50(5), 1790-1795.
Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology, 299, 152–178.
Sun, C., Wu, Z., Wang, Z., & Zhang, H. (2015). Effect of ethanol/water solvents on phenolic profiles and antioxidant properties of Beijing propolis extracts. Evidence-Based Complementary and Alternative Medicine, 2015.
Torres, A. R., Sandjo, L. P., Friedemann, M. T., Tomazzoli, M. M., Maraschin, M., Mello, C. F., & Santos, A. R. S. (2018). Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees. Brazilian Journal of Medical and Biological Research, 51(6).
Viuda-Martos, M., Ruiz-Navajas, Y., Fernández-López, J., & Pérez-Álvarez, J. A. (2008). Functional properties of honey, propolis, and royal jelly. Journal of food science, 73(9), R117-R124.
Yen, C. H., Chiu, H. F., Wu, C. H., Lu, Y. Y., Han, Y. C., Shen, Y. C., ... & Wang, C. K. (2017). Beneficial efficacy of various propolis extracts and their digestive products by in vitro simulated gastrointestinal digestion. LWT, 84, 281-289.
Yesiltas, B., Capanoglu, E., Firatligil-Durmus, E., Sunay, A. E., Samanci, T., & Boyacioglu, D. (2014). Investigating the in-vitro bioaccessibility of propolis and pollen using a simulated gastrointestinal digestion System. Journal of Apicultural Research, 53(1), 101-108.
Turkut, G. M., Er, M., & Degırmencı, A. (2019). Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared with Various Solvents. Journal of Apitherapy and Nature, 2(1), 7-11. https://doi.org/10.35206/jan.577616
AMA
Turkut GM, Er M, Degırmencı A. Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared with Various Solvents. J.Apit.Nat. November 2019;2(1):7-11. doi:10.35206/jan.577616
Chicago
Turkut, Gulsum Merve, Mehtap Er, and Atiye Degırmencı. “Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared With Various Solvents”. Journal of Apitherapy and Nature 2, no. 1 (November 2019): 7-11. https://doi.org/10.35206/jan.577616.
EndNote
Turkut GM, Er M, Degırmencı A (November 1, 2019) Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared with Various Solvents. Journal of Apitherapy and Nature 2 1 7–11.
IEEE
G. M. Turkut, M. Er, and A. Degırmencı, “Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared with Various Solvents”, J.Apit.Nat., vol. 2, no. 1, pp. 7–11, 2019, doi: 10.35206/jan.577616.
ISNAD
Turkut, Gulsum Merve et al. “Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared With Various Solvents”. Journal of Apitherapy and Nature 2/1 (November 2019), 7-11. https://doi.org/10.35206/jan.577616.
JAMA
Turkut GM, Er M, Degırmencı A. Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared with Various Solvents. J.Apit.Nat. 2019;2:7–11.
MLA
Turkut, Gulsum Merve et al. “Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared With Various Solvents”. Journal of Apitherapy and Nature, vol. 2, no. 1, 2019, pp. 7-11, doi:10.35206/jan.577616.
Vancouver
Turkut GM, Er M, Degırmencı A. Evaluating Bioactivity and Bioaccessibility Properties of Turkish Propolis Extracts Prepared with Various Solvents. J.Apit.Nat. 2019;2(1):7-11.
ASOS Index