Gastroprotective Effects of Jujuba (Ziziphus jujuba) Fruit Extract Against the Ethanol-Related Gastric Ulcer in Rats

Year 2023, Volume: 13 Issue: 6, 1046 - 1052, 30.11.2023

Ayhan Tanyeli , Mustafa Can Güler , Ersen Eraslan , Mehmet Ramazan Bozhüyük , Fazile Nur Ekinci Akdemir , Erdem Toktay , Nezahat Kurt , Gülay Özkan , Esra Çapanoğlu Güven

Abstract

Abstract
Objective: Gastric ulcer is a global health problem. Alcohol consumption and nonsteroidal anti-inflammatory drugs may lead to gastric ulcers. Here, we examined the possible gastroprotective effects of Ziziphus jujuba (Zj) fruit extract on a rat model of ethanol-induced gastric ulcer.
Material and Methods: Chemical properties of Zj were specified using performance liquid chromatography coupled to mass spectrometry. The animals were administered Zj extract (4-8 ml/kg) before administering absolute ethanol. Oxidative stress and proinflammatory markers, immunoexpression of NF-ĸB and caspase-3 were measured to evaluate Zj’s effects.
Results: Zj fruit extract significantly reduced values of cytokines such as TNF-α, IL-1β, and IL-6 and the expressions of NF-ĸB and caspase-3 in the immunohistologic analysis. We found out ulcerative foci in the ulcer group in the histopathological analysis, while we didn’t observe ulcerative foci in the treatment groups.
Conclusion: This experimental study showed that Zj fruit ameliorated the ethanol-induced gastric ulcer model in rats.

Keywords

Ethanol, gastric ulcer, proinflammatory cytokines, caspase-3, Ziziphus jujuba

Jujuba (Ziziphus jujuba) Meyve Ekstraktının Ratlarda Etanol ile Oluşturulan Mide Ülzerine Karşı Gastroprotektif Etkileri

Abstract

Öz

Amaç: Gastrik ülser global bir sağlık sorunudur. Alkol tüketimi ve nonsteroid anti-inflamatuar ilaç kullanımı mide ülserine yol açabilmektedir. Bu çalışmada, Ziziphus jujuba (Zj) meyve ekstraktının ratlarda oluşturdurğumuz etanol kaynaklı mide ülseri modeli üzerindeki olası gastroprotektif etkilerini inceledik.

Gereç ve Yöntem: Zj'nin kimyasal özellikleri kütle spektrometresi ile birleştirilmiş performans sıvı kromatografisi kullanılarak belirlendi. Hayvanlara saf etanol verilmeden önce Zj ekstresi (4-8 ml/kg) uygulandı. Zj'nin etkilerini değerlendirmek için oksidatif stres ve proinflamatuar belirteçler, NF-ĸB ve kaspaz-3'ün immünoekspresyonu ölçüldü.

Bulgular: Zj meyve ektraktı, immünohistolojik analizde TNF-α, IL-1β ve IL-6 gibi sitokinlerin değerlerini ve NF-ĸB ve kaspaz-3 ekspresyonlarını önemli ölçüde azaltmıştır. Histopatolojik analizde ülser grubunda ülseratif odaklar tespit edilirken, tedavi gruplarında ülseratif odaklar gözlenmedi.

Sonuç: Bu deneysel çalışma, Zj meyvesinin sıçanlarda etanol ile indüklenen gastrik ülser modelini iyileştirdiğini göstermiştir.

Keywords

Etanol, gastrik ülser, proinflamatuar sitokinler, kaspaz-3, Ziziphus jujuba

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