Demonstrating Distribution in Turkey Arctosa maculata (Hahn, 1822) (Araneae: Lycosidae) Type of Investigation of Karyological Features

Abstract

In this study, karyotype analysis of Arctosa maculata (Hahn, 1822) species in Lycosidae family was performed for the first time in our country. Standard giemsa staining method was applied to obtain chromosomes. This method includes four main steps: obtaining gonads, hypotonic application, fixation and staining. In the study, the number of diploid chromosomes belonging to the species was determined as 2n♂ = 28. The sex chromosome system is X1X20; all chromosomes are of telosentric type. It has been noted that the relative lengths of the autosomal chromosomes gradually decrease. It was determined that sex chromosomes have positive heteropicnotic features in meiosis I phases and isopicnotic features in meiosis II phases.

Keywords

• Lycosidae
• Karyotype
• Sex chromosome

Türkiye’de Yayılış Gösteren Arctosa maculata (Hahn, 1822) (Araneae: Lycosidae) Türünün Karyolojik Özelliklerinin Araştırılması

Abstract

Bu çalışmada Lycosidae familyası içerisinde yer alan Arctosa maculata (Hahn, 1822) türünün karyotip analizi ülkemizde ilk kez yapılmıştır. Kromozomların elde edilmesinde standart giemsa boyama yöntemi uygulanmıştır. Bu yöntem, gonadların elde edilmesi, hipotonik uygulama, fiksasyon ve boyama olmak üzere dört ana basamak içermektedir. Çalışmada türe ait diploid kromozom sayısı 2n♂=28 olarak tespit edilmiştir. Eşey kromozomu sistemi X1X20 olup; tüm kromozomlar telosentrik tiptedir. Otozomal kromozomların relatif uzunluklarının kademeli olarak bir azalış gösterdiği kaydedilmiştir. Eşey kromozomlarının mayoz I evrelerinde pozitif heteropiknotik özellikte, mayoz II evrelerinde ise izopiknotik özellikte oldukları belirlenmiştir.

Keywords

• Lycosidae
• Karyotip
• Eşey kromozomu

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