Antioxidant Enzyme, Phenolic Substance and Plant Nutrient Contents of Malva sylvestris L. and Alcea rosea L. Species Used as Medicinal Plants

Year 2021, Volume: 11 Issue: 1, 786 - 794, 01.03.2021

Şaban Kordalı , Ayşe Usanmaz Bozhüyük , Erman Beyzi , Adem Güneş , Metin Turan

https://doi.org/10.21597/jist.747673

Cited By: 4

Abstract

It is of great importance to know the antioxidant, phenolic and nutrient content of medicinal and aromatic plants in order to evaluate different usage areas. For this purpose, antioxidant enzyme activity and total antioxidant, phenolic substance and plant nutrient content of Malva sylvestris L. and Alcea rosea L. plants of Malvaceae family were determined in this study. As a result of the study, the highest peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (AxPOD) enzyme activity were measured in Alcea rosea L. species. Total carotenoid and total phenolic substance amount were higher in Alcea rosea L. species than Malva sylvestris L. species. When the nutrient content of the plants is evaluated, the highest N, P, Na, Fe and Mn amount were determined in the Alcea rosea L. species, and the highest K, Ca, Mg, Zn and Cu amount were determined in the Malva sylvestris L. species. According to these results, it is determined that Alcea rosea L. species, which is rich in antioxidant, phenolic substance and total carotenoid amounts and some plant nutrient amounts, has higher usage potential in different areas.

Keywords

Malvaceae, medicinal plant, antioxidant enzyme

Tıbbi Bitki Olarak Kullanılan Malva sylvestris L. ve Alcea rosea L. Türlerinin Antioksidant Enzim, Fenolik Madde ve Bitki Besin Element İçerikleri

Abstract

Tıbbi ve aromatik bitkilerin, farklı kullanım alanlarını değerlendirebilmek için içerdikleri antioksidan, fenolik ve besin element miktarlarının bilinmesi büyük önem arz etmektedir. Bu amaçla bu çalışmada, Malvaceae familyasına ait, Malva sylvestris L. ve Alcea rosea L. bitkilerinin antioksidan enzim aktivitesi ile toplam antioksidan, fenolik madde ve bitki besin element içerikleri belirlenmiştir. Çalışma sonucunda, en yüksek peroksidaz (POD), katalaz (CAT), süperoksid dismutaz (SOD) ve askorbat peroksidaz (AxPOD) enzim aktivitesi Alcea rosea L. bitkisinde ölçülmüştür. Toplam karotenoid ve toplam fenolik madde miktarı Malva sylvestris L. bitkisine göre Alcea rosea L. bitkisinde daha yüksek ölçülmüştür. Bitkilerin besin içerikleri değerlendirildiğinde en yüksek N, P, Na, Fe ve Mn miktarı Alcea rosea L. bitkisinde belirlenirken, K, Ca, Mg, Zn ve Cu miktarı ise en yüksek Malva sylvestris L. bitkisinde belirlenmiştir. Bu sonuçlara göre antioksidan, fenolik madde ve toplam karotenoid miktarları ile bazı bitki besin madde miktarları bakımından zengin olan Alcea rosea L. bitkisinin, farklı alanlarda kullanım potansiyelinin daha yüksek olduğu belirlenmiştir.

Keywords

Malvaceae, tıbbi bitki, antioksidan enzim

References

• Abdel-salam NA, Ghazy NM, Sallam SM, Radwan MM, Wanas AS, ElSohly MA, El-Demellawy MA, Abdel-Rahman NM, Piacente S, Shenouda ML, 2018. Flavonoids of Alcea rosea L. and their immune stimulant, antioxidant and cytotoxic activities on hepatocellular carcinoma HepG-2 cell line, Natural Product Research, 32(6):702-706.
• Ammar NM, El-Kashoury SA, Abou El-Kassem LT, Abd El-Hakeem RE, 2013. Evaluation of the Phenolic Content and Antioxidant Potential of Althaea rosea Cultivated in Egypt, Journal of the Arab Society for Medical Research, 8:48–52.
• Azizov UM, Mirakilova DB, Umarova NT, Salikhov SA, Rakhimov DA, Mezhlumyan LG, 2007. Chemıcal Composıtıon of Dry Extracts From Alcea rosea, Chemistry of Natural Compounds, 43 (5):508-511.
• Barros L, Carvalho AM, Ferreira IC, 2010. Leaves, flowers, immature fruits and leafy flowered stems of Malva sylvestris: A comparative study of the nutraceutical potential and composition. Food Chem Toxicol, 48(6):1466-72.
• Beghdad MC, Benammar C, Bensalah F, Sabri FZ, Belarbi M, Chemat F, 2014. Antioxidant activity, phenolic and flavonoid content in leaves, flowers, stems and seeds of mallow (Malva sylvestris L.) from North Western of Algeria. African Journal of Biotechnology, 13 (3): 486-491.
• Bremner JM, 1996. Nitrogen-total. In: Bartels JM, Bigham JM (eds)Chemical methods. Methods of soil analysis, vol 3. The Soil Sci-ence Society of America and the American Society of Agronomy,Madison, pp: 1085–112.
• Büyükbayram F, 2019. Diyarbakır İli’ne Ait Bazı Alcea L. Türlerinin Morfolojik ve Anatomik Özellikleri. Dicle Üniversitesi Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Yüksek Lisans Tezi, Diyarbakır.
• Cai YZ, Luo Q, Sun M, Corke H, 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Science, 74: 2157-2184.
• Cakmak I, Horst J, 1991. Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum 83: 463–468.
• Cutillo F, D’Abrosca B, Dellagreca M, Fiorentino A, Zarrelli A. 2006. Terpenoides and phenol derivatives from Malva sylvestris. Phytochemistry, 67:481-485.
• Davies P.H. 1988. Flora of Turkey and the East Aegean Islands. Edinburgh University Press. pp. 590.
• Dragland S, Senoo H, Wake K, Holte K, Blomhoff R. 2003. Several culinary and medicinal herbs are important sources of dietary antioxidants. Journal of Nutrition, 133: 1286-1290.
• Du B, Nian H, Zhang Z, Yang C, 2010. Effects of aluminum on superoxide dismutase and peroxidase activities, and lipid peroxidation in the roots and calluses of soybeans differing in aluminum tolerance. Acta Physiologia Plantarum 32:883–890.
• Dudek M, Matlawska I, Szkudlarek M, 2006. Phenolıc Acıds ın The Flowers of Althaea rosea var. Nigra, Acta Poloniae Pharmaceutica-Drug Research, 63 (3): 207-211.
• Fagbohun E, Asare RR, Egbebi AO, 2012. Chemical Composition and Antimicrobial Activities of Urena lobata L.(Malvaceae). Journal of Medicinal Plants Research, 6(12):2256-2260.
• Fersahoğlu H, 2016. Farklı Renklerdeki Gülhatmi Çiçeklerinin Biyoaktif Özellikleri. Yüksek Lisans Tezi, Yıldız Teknik Üniversitesi, Fen Bilimleri Enstitüsü, İstanbul.
• Filiz EB, Seydim AC, 2014. Bazı Kurutulmuş Meyvelerin Antioksidan Özellikleri, Türk Tarım – Gıda Bilim ve Teknoloji Dergisi, 2(3):128- 131.
• Güncan A, 1997. Yabancı otların tıbbi ilaçlar açısından önemi. Türkiye II. Herboloji Kongresi. 1-4 Eylül 1997 İzmir & Ayvalık Bildiriler, 147-152.
• Katsube T, Tabata H, Ohta Y, Yamasaki Y, Anuurad E, Shiwaku K, Yamane Y, 2004. Screening for antioxidant activity in edible plant products: comparison of lowdensity lipoprotein oxidation assay, DPPH Radical Scavenging Assay, and Folin−Ciocalteu Assay. Journal of Agricultural and Food Chemistry 52 (8): 2391–2396.
• Kaya İ, İncekara N, Nemli Y, 2004. Ege Bölgesi’nde Sebze Olarak Tüketilen Yabani Kuşkonmaz, Sirken, Yabani Hindiba, Rezene, Gelincik, Çoban Değneği ve Ebegümecinin Bazı Kimyasal Analizleri. Yüzüncü Yıl Üniversitesi Ziraat Fakültesi Tarım Bilimleri Dergisi, 14 (1): 1-6
• Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, Feng Y, 2015. The role of oxidative stress and antioxidants in liver diseases. International Journal of Molecular Sciences 16:26087–26124.
• Lichtenthaler HK, Wellburn AR, 1985. Determination of Total Carotenoids and Chlorophylls A and B of Leaf in Different Solvents. Biol. Soc. Trans 11: 591-592.
• Lim T, 2014. Alcea rosea. In: Edible medicinal and non medicinal plants. vol. 8. Netherlands: Springer; p. 292–299
• Loreto F, Velikova V, 2001. Isoprene produced by leaves protects the photosynthetic apparatus against ozone damage, quenches ozone products, and reduces lipid peroxidation of cellular membranes. Plant Physiology 127: 1781–1787.
• Marouane W, Soussi A, Murat JC, Bezzine S, El Feki A. 2011. The protective effect of Malva sylvestris on rat kidney damaged by vanadium. Lipids Health Dis., 10:65.
• Mertens D, 2005a. AOAC Official Method 922,02, Plants Preparation of Laboratuary Sample, Official Methods of Analysis, 18th edn, Horwitz, W,, and G,W, Latimer, (Eds), Chapter 3, pp1-2, AOAC-International Suite 500, 481, North Frederick Avenue, Gaitherburg, Maryland 20877-2417, USA.
• Mertens D, 2005b. AOAC Official Method 975,03, Metal in Plants and Pet Foods, Official Methods of Analysis, 18th edn, Horwitz, W,, and G,W, Latimer, (Eds), Chapter 3, pp 3- 4, AOAC-International Suite 500, 481, North Frederick Avenue, Gaitherburg, Maryland 20877-2417, USA.
• Prieto P, Pineda M, Aguilar M, 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphor molybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry 269(2): 337–341.
• Pytlakowska K, Kita A, Janoska P, Połowniak M, Kozik V, 2012. Multi-Element Analysis of Mineral and Trace Elements in Medicinal Herbs and Their Infusions. Food Chemistry, 135(2):494-501.
• Rao KS, Lakshminarayana G, 1984. Characteristics and Composition of Six Malvaceae Seeds and the Oils. Journal of the American Oil Chemists' Society, 61(8): 1345-1346.
• Sairam RK, Srivastava GC, 2002. Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Science 162:897-904.
• Schinella GR, Tournier HA, Prieto JM, Mordujovich de Buschiazzo P, Rios JL. 2002. Antioxidant activity of anti-inflammatory plant extracts. Life Science, 70: 1023-1033.
• Song FL, Gan RY, Zhang Y, Xiao Q, Kuang L, Li HB. 2010. Total Phenolic Contents and Antioxidant Capacities of Selected Chinese Medicinal Plants. International Journal of Molecular Sciences, 11: 2362-2372.
• Spiridon I, Bodirlau R, Teaca CA, 2011. Total phenolic content and antioxidant activity of plants used in traditional Romanian herbal medicine. Central European Journal of Biology 6(3):388–396.
• Tabaraki R, Yosef Z, Gharneh HAA, 2012. Chemical Composition and Antioxidant Properties of Malva sylvestris L. Journal of Research in Agricultural Science 8(1):59 – 68.
• Tan S, 2016. Malope malacoides ve Malva sylvestris (Malvaceae) Türlerinin Morfolojik, Anatomik, Palinolojik ve Ekolojik Yönden İncelenmesi. Celal Bayar Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı, Yüksek Lisans Tezi, Manisa.
• Veshkorova O, Golubenko Z, Pshenichnov E, Avzanov I, Uzbekov V, Sultanova E, Shavkat S., Williams HJ, Reibenspies JH, Puckhaber LS, Stipanovic RD, 2010. Malvone A, a phytoalexin found in Malva sylvestris (Family Malvaceae). Phytochemistry ; 67:2376-2379.