Year 2021, Volume 11 , Issue 2, Pages 869 - 879 2021-06-01

Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması
Investigation of Fatty Acid Composition and Therapeutic Potential of Methanol Extract of Agaricus campestris

Mehmet Kadir ERDOĞAN [1] , Can Ali AGCA [2] , İbrahim Halil GEÇİBESLER [3]


Yapılan bilimsel araştırmalar mantarların çok umut verici farmakolojik potansiyele sahip olduğuna işaret etmektedir. Agaricus campestris, önemli biyoaktiviteye sahip yenilebilir bir mantar türüdür. Bu çalışmada, A. campestris metanol ekstraktının (AC) antioksidan ve antiproliferatif aktiviteleri ile yağ asidi kompozisyonunun değerlendirilmesi amaçlanmıştır. AC içeriğindeki yağ asitlerinin kantitatif analizi GC-MS spektrometresi ile değerlendirilmiştir. Antioksidan aktivitenin belirlemesi için ise DPPH serbest radikali giderme testi, ABTS katyon radikali giderme aktivitesi, ferrik iyonlarını indirgeme testi ve Ferröz iyonlarını şelatlama testleri kullanılmıştır. AC ekstraktının antiproliferatif etkisi ise insan kolon (HT-29), meme (MCF-7) ve beyin (U373) kanseri hücre hatlarında zaman ve doz bağımlı olarak WST-1 hücre canlılığı analizi ile belirlenmiştir. AC içeriğinde baskın bileşenler olarak doymamış yağ asidi olan linoleik asit (18:2, ω-6) %56.45 oranında ve doymuş yağ asidi olan palmitik asit (16:0) %28.74 oranında analiz edilmiştir. Antioksidan aktivite parametreleri geniş konsantrasyon aralıklarında değerlendirildiğinde, artan konsantrasyon ile lineer bir antioksidan aktivite artışı gözlendi. AC 24 saatlik tedavide HT-29 hücrelerinde 52.62 μg mL-1 IC50 değeri ile en yüksek antiproliferatif etkiyi göstermiştir. Bu bulgular, A. campestris hakkında daha fazla araştırma yapmak için ilginç veriler ortaya koymaktadır, ve özellikle kanser hücreleri üzerindeki güçlü antiproliferatif potansiyel mantarın daha fazla araştırılmaya değer olduğunu göstermektedir.
Recent reports indicate that mushrooms have very promising pharmacological potential. Agaricus campestris is an edible fungus species with bio-activity. This study aimed to screen the anti-oxidant, anti-proliferative activities, and fatty acid composition of methanol extract of A. campestris (AC). The quantitative analysis of fatty acids of AC was evaluated by GC-MS spectrometer. For the determination of antioxidant activity, DPPH free radical scavenging test, ABTS radical scavenging activity, Ferric ions reduction assay and Ferrous ions chelation assays were used. The antiproliferative effect of AC was determined by the time and dose dependent with WST-1 cell viability analysis in human colon (HT-29), breast (MCF-7) and brain (U373) cancer cell lines. Linoleic acid (18: 2, ω-6), which is the unsaturated fatty acid, was analyzed at 56.45% and the saturated fatty acid palmitic acid (16: 0) was analyzed at 28.74% as the major components in AC. It was observed that AC, whose antioxidant activity parameters were evaluated in wide concentration ranges, displayed a linear increase in antioxidant activity with increasing concentration. AC showed the highest antiproliferative effect in HT-29 cells with an IC50 value of 52.62 μg mL-1 for 24 h treatment. These results provide interesting data for further research on A. campestris, and its strong antiproliferative potential particularly on cancer cells, indicates that the mushroom is worth further investigation.
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Primary Language tr
Subjects Biology
Published Date Haziran-2021
Journal Section Biyoloji / Biology
Authors

Orcid: 0000-0002-1579-5737
Author: Mehmet Kadir ERDOĞAN (Primary Author)
Institution: Bingol University, Faculty of Arts and Science, Department of Biology, 12000, Bingöl, Turkey
Country: Turkey


Orcid: 0000-0002-0244-3767
Author: Can Ali AGCA
Institution: Bingol University, Faculty of Arts and Science, Department of Molecular Biology and Genetics, Bingöl, Turkey
Country: Turkey


Orcid: 0000-0002-4473-2671
Author: İbrahim Halil GEÇİBESLER
Institution: Bingol University, Faculty of Health Science, Department of Occupational Health and Safety, Laboratory of Natural Product Research, 12000, Bingöl, Turkey
Country: Turkey


Supporting Institution Bingöl Üniversitesi
Project Number BAP-FEF.2017.00.010
Thanks Bu çalışma BAP-FEF.2017.00.010 proje numarası ile Bingöl Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir.
Dates

Application Date : July 16, 2020
Acceptance Date : January 4, 2021
Publication Date : June 1, 2021

Bibtex @research article { jist769529, journal = {Journal of the Institute of Science and Technology}, issn = {2146-0574}, eissn = {2536-4618}, address = {}, publisher = {Igdir University}, year = {2021}, volume = {11}, pages = {869 - 879}, doi = {10.21597/jist.769529}, title = {Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması}, key = {cite}, author = {Erdoğan, Mehmet Kadir and Agca, Can Ali and Geçibesler, İbrahim Halil} }
APA Erdoğan, M , Agca, C , Geçibesler, İ . (2021). Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması . Journal of the Institute of Science and Technology , 11 (2) , 869-879 . DOI: 10.21597/jist.769529
MLA Erdoğan, M , Agca, C , Geçibesler, İ . "Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması" . Journal of the Institute of Science and Technology 11 (2021 ): 869-879 <https://dergipark.org.tr/en/pub/jist/issue/61423/769529>
Chicago Erdoğan, M , Agca, C , Geçibesler, İ . "Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması". Journal of the Institute of Science and Technology 11 (2021 ): 869-879
RIS TY - JOUR T1 - Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması AU - Mehmet Kadir Erdoğan , Can Ali Agca , İbrahim Halil Geçibesler Y1 - 2021 PY - 2021 N1 - doi: 10.21597/jist.769529 DO - 10.21597/jist.769529 T2 - Journal of the Institute of Science and Technology JF - Journal JO - JOR SP - 869 EP - 879 VL - 11 IS - 2 SN - 2146-0574-2536-4618 M3 - doi: 10.21597/jist.769529 UR - https://doi.org/10.21597/jist.769529 Y2 - 2021 ER -
EndNote %0 Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması %A Mehmet Kadir Erdoğan , Can Ali Agca , İbrahim Halil Geçibesler %T Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması %D 2021 %J Journal of the Institute of Science and Technology %P 2146-0574-2536-4618 %V 11 %N 2 %R doi: 10.21597/jist.769529 %U 10.21597/jist.769529
ISNAD Erdoğan, Mehmet Kadir , Agca, Can Ali , Geçibesler, İbrahim Halil . "Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması". Journal of the Institute of Science and Technology 11 / 2 (June 2021): 869-879 . https://doi.org/10.21597/jist.769529
AMA Erdoğan M , Agca C , Geçibesler İ . Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması. Iğdır Üniv. Fen Bil Enst. Der.. 2021; 11(2): 869-879.
Vancouver Erdoğan M , Agca C , Geçibesler İ . Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması. Journal of the Institute of Science and Technology. 2021; 11(2): 869-879.
IEEE M. Erdoğan , C. Agca and İ. Geçibesler , "Agaricus campestris Metanol Ekstraktının Yağ Asidi Kompozisyonu ve Terapötik Potansiyelinin Araştırılması", Journal of the Institute of Science and Technology, vol. 11, no. 2, pp. 869-879, Jun. 2021, doi:10.21597/jist.769529