Year 2021, Volume 11 , Issue 2, Pages 916 - 926 2021-06-01

Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi
Antiangiogenic and Cytotoxic Activity of Giresun Tombul Hazelnut (Corylus avellana L.) Oil on Cervix, Breast, and Colon Cancer Cells

Adem GÜNER [1] , Özlem GÜNER [2] , Ülkü KARABAY [3]


Corylus avellana L. çoğunlukla Giresun’da yetişen dünyanın en kaliteli fındık türlerinden biridir. Bu çalışma, Giresun Tombul fındık yağının (0.5, 5 ve 50 mg L-1) antikanser ve antianjiyojenik aktivitelerini değerlendirmek amacıyla gerçekleştirilmiştir. Sitotoksisite insan serviks, kolon ve meme kanseri hücreleri üzerinde MTT ve laktat dehidrojenaz salım (LDH) analizleri ile belirlenmiştir. Hücrelerin oksidatif durumu, toplam oksidatif stres (TOS) ve toplam antioksidan kapasite (TAK) yöntemleriyle ortaya konulmuştur. Anti-anjiyojenik aktiviteyi değerlendirmek için civciv koryoallantoik membran (HET-CAM) deneyi kullanılmıştır. Sonuçlarımız, C. avellana yağının özellikle serviks kanseri hücrelerinde daha fazla olmak üzere, serviks ve kolon kanseri hücrelerinin (sırasıyla IC 50 = 6.5 ± 0.35 mg L-1 ve IC 50 = 26.2 ± 3.15 mg L-1) canlılığını önemli ölçüde inhibe ettiğini göstermiştir. Bununla birlikte, fındık yağ örneği, LDH salınımında önemli bir inhibisyona yol açarak sitotoksisite ile doğrusal bir korelasyon sergilemiştir. C. avellana yağ örneği konsantrasyona bağlı bir şekilde serviks ve kolon hücrelerinin TAK seviyelerinde önemli bir artışa neden olurken, TOS seviyelerinde istatistiksel olarak önemli azalmaya yol açmıştır. Aynı zamanda, C. avellana yağ örneği yüksek konsantrasyonlarda anti-anjiyojenik etkinlik göstermiştir. Sonuçlar, Giresun fındığının umut verici bir antikanser ajan olduğunu ve bununla birlikte terapötik etkinliğini ortaya koymak için daha ileri çalışmalara ihtiyaç olduğunu göstermiştir.
Corylus avellana L. is one of the best quality hazelnut species grown mostly in Giresun province. This present study was realised to evaluate the anticancer and antiangiogenic activities of Giresun Tombul hazelnut oil (0.5, 5 and 50 mg L-1). Cytotoxicity was determined by MTT and lactate dehydrogenase release (LDH) assays on human cervix, colon, and breast cancer cells. Oxidative status was analyzed by total oxidative stress (TOS) and total antioxidant capacity (TAC) assay. Hen’s egg test chorioallantoic membrane (HET-CAM) assay was used to evaluate the anti-angiogenic activity. Our results showed that C. avellana oil had significant anti-proliferative activity against both cervix and colon cancer cells (6.5 ± 0.35 mg L-1 and 26.26 ± 3.15 mg L-1, respectively), with more inhibiting the cervix cancers and caused significant inhibition in LDH release as a linear correlation with MTT results. C. avellana oil significantly increased the TAC status on the cervix and colon cells while causing a significant reduction in TOS levels in a dose-dependent manner. C. avellana oil had anti-angiogenic effectiveness in higher doses. The results indicated that Giresun hazelnut was a promising anticancer agent and however, further studies are needed to reveal its therapeutic effectiveness.
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Primary Language tr
Subjects Biology
Published Date Haziran-2021
Journal Section Biyoloji / Biology
Authors

Orcid: 0000-0003-3295-3538
Author: Adem GÜNER (Primary Author)
Institution: SİNOP ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-8302-9073
Author: Özlem GÜNER
Institution: SİNOP ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-7483-0184
Author: Ülkü KARABAY
Institution: EGE ÜNİVERSİTESİ
Country: Turkey


Supporting Institution Giresun Üniversitesi
Project Number FEN-BAP-A-150219-28
Dates

Application Date : October 24, 2020
Acceptance Date : February 26, 2021
Publication Date : June 1, 2021

Bibtex @research article { jist815965, journal = {Journal of the Institute of Science and Technology}, issn = {2146-0574}, eissn = {2536-4618}, address = {}, publisher = {Igdir University}, year = {2021}, volume = {11}, pages = {916 - 926}, doi = {10.21597/jist.815965}, title = {Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi}, key = {cite}, author = {Güner, Adem and Güner, Özlem and Karabay, Ülkü} }
APA Güner, A , Güner, Ö , Karabay, Ü . (2021). Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi . Journal of the Institute of Science and Technology , 11 (2) , 916-926 . DOI: 10.21597/jist.815965
MLA Güner, A , Güner, Ö , Karabay, Ü . "Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi" . Journal of the Institute of Science and Technology 11 (2021 ): 916-926 <https://dergipark.org.tr/en/pub/jist/issue/61423/815965>
Chicago Güner, A , Güner, Ö , Karabay, Ü . "Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi". Journal of the Institute of Science and Technology 11 (2021 ): 916-926
RIS TY - JOUR T1 - Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi AU - Adem Güner , Özlem Güner , Ülkü Karabay Y1 - 2021 PY - 2021 N1 - doi: 10.21597/jist.815965 DO - 10.21597/jist.815965 T2 - Journal of the Institute of Science and Technology JF - Journal JO - JOR SP - 916 EP - 926 VL - 11 IS - 2 SN - 2146-0574-2536-4618 M3 - doi: 10.21597/jist.815965 UR - https://doi.org/10.21597/jist.815965 Y2 - 2021 ER -
EndNote %0 Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi %A Adem Güner , Özlem Güner , Ülkü Karabay %T Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi %D 2021 %J Journal of the Institute of Science and Technology %P 2146-0574-2536-4618 %V 11 %N 2 %R doi: 10.21597/jist.815965 %U 10.21597/jist.815965
ISNAD Güner, Adem , Güner, Özlem , Karabay, Ülkü . "Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi". Journal of the Institute of Science and Technology 11 / 2 (June 2021): 916-926 . https://doi.org/10.21597/jist.815965
AMA Güner A , Güner Ö , Karabay Ü . Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi. Iğdır Üniv. Fen Bil Enst. Der.. 2021; 11(2): 916-926.
Vancouver Güner A , Güner Ö , Karabay Ü . Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi. Journal of the Institute of Science and Technology. 2021; 11(2): 916-926.
IEEE A. Güner , Ö. Güner and Ü. Karabay , "Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi", Journal of the Institute of Science and Technology, vol. 11, no. 2, pp. 916-926, Jun. 2021, doi:10.21597/jist.815965