Year 2021, Volume 11 , Issue 2, Pages 1321 - 1328 2021-06-01

Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila
Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila

Özgenur DİNÇER [1] , Hasan Ufuk CELEBİOGLU [2] , Attia HAMID [3] , Muhammad Nauman AFTAB [4] , Ahmet KARADAĞ [5]


Enzymes can be used in various biotechnological applications due to the easy and cheap production. Since xylanase enzymes are preferred in various industries, researchon this enzyme is extensively being carried out. In this study, the β-xylanase gene was cloned from Thermotoga naphthophila, a thermophilic organism. The expression vector pET21a(+) was expressed in Escherichia coli BL21 (DE3). As a result of the studies, the pH, temperature and IPTG concentration of the enzyme were optimized to obtain highest expression. Dinitrosalicylic acid (DNS) was used to determine sugar content of the enzyme. The molecular mass of the purified β-xylanase enzyme was determined using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The molecular mass of the enzyme was calculated to be 38 kDa. Enzymatic hydrolysis of hazelnut shell, rhododendron branch and rhododendron leaves was performed. Released reducing sugar contents from the enzymatic hydrolysis were calculated as 0.8461 mg mL-1, 0.6976 mg mL-1 and 0.3605 mg mL-1 for hazelnut shell, rhododendron branch, and rhododendron leaf respectively. In conclusion, β-xylanase enzyme can be an effective source for enzymatic hydrolysis to produce fermentable sugars from such biomasses.
Enzymes can be used in various biotechnological applications due to the easy and cheap production. Since xylanase enzymes are preferred in various industries, researchon this enzyme is extensively being carried out. In this study, the β-xylanase gene was cloned from Thermotoga naphthophila, a thermophilic organism. The expression vector pET21a(+) was expressed in Escherichia coli BL21 (DE3). As a result of the studies, the pH, temperature and IPTG concentration of the enzyme were optimized to obtain highest expression. Dinitrosalicylic acid (DNS) was used to determine sugar content of the enzyme. The molecular mass of the purified β-xylanase enzyme was determined using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The molecular mass of the enzyme was calculated to be 38 kDa. Enzymatic hydrolysis of hazelnut shell, rhododendron branch and rhododendron leaves was performed. Released reducing sugar contents from the enzymatic hydrolysis were calculated as 0.8461 mg mL-1, 0.6976 mg mL-1 and 0.3605 mg mL-1 for hazelnut shell, rhododendron branch, and rhododendron leaf respectively. In conclusion, β-xylanase enzyme can be an effective source for enzymatic hydrolysis to produce fermentable sugars from such biomasses.
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Primary Language en
Subjects Engineering, Chemical
Published Date Haziran-2021
Journal Section Kimya / Chemistry, Kimya Mühendisliği / Chemical Engineering
Authors

Orcid: 0000-0002-2598-4865
Author: Özgenur DİNÇER
Institution: BARTIN UNIVERSITY
Country: Turkey


Orcid: 0000-0001-7207-2730
Author: Hasan Ufuk CELEBİOGLU (Primary Author)
Institution: BARTIN UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOTECHNOLOGY
Country: Turkey


Orcid: 0000-0003-4226-180X
Author: Attia HAMID
Institution: Government College University Lahore
Country: Pakistan


Orcid: 0000-0002-6224-8496
Author: Muhammad Nauman AFTAB
Institution: Government College University Lahore
Country: Pakistan


Orcid: 0000-0003-4676-683X
Author: Ahmet KARADAĞ
Institution: Yozgat Bozok University
Country: Turkey


Thanks This project was supported by PAK-Turk Collaborative Research Program of Turkish Council of Higher Education (YÖK).
Dates

Application Date : November 17, 2020
Acceptance Date : January 25, 2021
Publication Date : June 1, 2021

Bibtex @research article { jist827308, journal = {Journal of the Institute of Science and Technology}, issn = {2146-0574}, eissn = {2536-4618}, address = {}, publisher = {Igdir University}, year = {2021}, volume = {11}, pages = {1321 - 1328}, doi = {10.21597/jist.827308}, title = {Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila}, key = {cite}, author = {Dinçer, Özgenur and Celebioglu, Hasan Ufuk and Hamıd, Attia and Aftab, Muhammad Nauman and Karadağ, Ahmet} }
APA Dinçer, Ö , Celebioglu, H , Hamıd, A , Aftab, M , Karadağ, A . (2021). Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila . Journal of the Institute of Science and Technology , 11 (2) , 1321-1328 . DOI: 10.21597/jist.827308
MLA Dinçer, Ö , Celebioglu, H , Hamıd, A , Aftab, M , Karadağ, A . "Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila" . Journal of the Institute of Science and Technology 11 (2021 ): 1321-1328 <https://dergipark.org.tr/en/pub/jist/issue/61423/827308>
Chicago Dinçer, Ö , Celebioglu, H , Hamıd, A , Aftab, M , Karadağ, A . "Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila". Journal of the Institute of Science and Technology 11 (2021 ): 1321-1328
RIS TY - JOUR T1 - Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila AU - Özgenur Dinçer , Hasan Ufuk Celebioglu , Attia Hamıd , Muhammad Nauman Aftab , Ahmet Karadağ Y1 - 2021 PY - 2021 N1 - doi: 10.21597/jist.827308 DO - 10.21597/jist.827308 T2 - Journal of the Institute of Science and Technology JF - Journal JO - JOR SP - 1321 EP - 1328 VL - 11 IS - 2 SN - 2146-0574-2536-4618 M3 - doi: 10.21597/jist.827308 UR - https://doi.org/10.21597/jist.827308 Y2 - 2021 ER -
EndNote %0 Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila %A Özgenur Dinçer , Hasan Ufuk Celebioglu , Attia Hamıd , Muhammad Nauman Aftab , Ahmet Karadağ %T Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila %D 2021 %J Journal of the Institute of Science and Technology %P 2146-0574-2536-4618 %V 11 %N 2 %R doi: 10.21597/jist.827308 %U 10.21597/jist.827308
ISNAD Dinçer, Özgenur , Celebioglu, Hasan Ufuk , Hamıd, Attia , Aftab, Muhammad Nauman , Karadağ, Ahmet . "Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila". Journal of the Institute of Science and Technology 11 / 2 (June 2021): 1321-1328 . https://doi.org/10.21597/jist.827308
AMA Dinçer Ö , Celebioglu H , Hamıd A , Aftab M , Karadağ A . Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila. Iğdır Üniv. Fen Bil Enst. Der.. 2021; 11(2): 1321-1328.
Vancouver Dinçer Ö , Celebioglu H , Hamıd A , Aftab M , Karadağ A . Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila. Journal of the Institute of Science and Technology. 2021; 11(2): 1321-1328.
IEEE Ö. Dinçer , H. Celebioglu , A. Hamıd , M. Aftab and A. Karadağ , "Saccharification of Hazelnut and Rhododendron Biomasses Using β-xylanase from Thermotoga naphthophila", Journal of the Institute of Science and Technology, vol. 11, no. 2, pp. 1321-1328, Jun. 2021, doi:10.21597/jist.827308