Year 2021, Volume 11 , Issue 2, Pages 1402 - 1407 2021-06-01

Deneysel Florozis Oluştulmuş Ratlarda Kitosan ve Kitosan Oligosakkaridin Serum ve Doku Siyalik Asit Düzeyleri Üzerindeki Etkileri
Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis

Ugur OZDEK [1] , Yeter DEĞER [2]


Bu çalışmada deneysel florozisli ratlarda, kitosan (CH) ve kitosan oligosakkaritin (COS), serum ve dokularda (karaciğer, böbrek, beyin ve testis) toplam siyalik asit (TSA) düzeyine etkisi araştırılmıştır. Gruplar kontrol, sodyum florür (NaF), NaF + kitosan (NaF + CH), NaF + kitosan oligosakkarit (NaF + COS), kitosan (CH) ve kitosan oligosakkarit (COS) olarak oluşturuldu. NaF gruplarının içme suyu 100 ppm sodyum florür konsantrasyonunda hazırlandı. Deney gruplarında, doksan gün süreyle kitosan ve kitosan oligosakkarit 250 mg/kg dozunda oral yolla uygulandı. Çalışma sonunda serum ve karaciğer, böbrek, beyin ve testis doku homojenizatlarının TSA düzeyi spektrofotometrik yöntemle belirlendi. Florür uygulanan grupta (NaF), kontrol grubu ile karşılaştırıldığında, serum, karaciğer, böbrek, beyin ve testis dokularında TSA düzeylerinin arttığı görüldü (p<0.05). NaF grubuna göre, NaF+CH grubunda serum seviyelerinde, NaF+COS grubunda ise serum, karaciğer ve böbrek dokularında belirgin düşüş vardı. Beyin dokusu sialik asit düzeyi açısından kontrol ve deney grupları arasında fark olmadığı belirlendi (p>0.05). Sonuç olarak, flor toksikasyonunun, serum ve dokularda hücre hasarına neden olarak, TSA düzeylerinde artışa neden olduğu düşünülebilir. Sunulan çalışmada, CH ve COS'un TSA seviyelerini düşürdüğü gösterilmiştir. Ayrıca bu çalışmada, COS'un TSA seviyesini azaltmada daha etkili olduğu görüldü.
In this study, the effect of chitosan (CH) and chitosan oligosaccharide (COS) on serum and tissue (liver, kidney, brain and testis) total sialic acid (TSA) level was investigated in rats with experimental fluorosis. The groups were formed as control, sodium fluoride (NaF), NaF+chitosan (NaF+CH), NaF+chitosan oligosaccharide (NaF+COS), chitosan (CH) and chitosan oligosaccharide (COS). Drinking water of NaF groups was prepared at a concentration of 100 ppm sodium fluoride. Chitosan and chitosan oligosaccharide were given to Experimental groups as 250 mg/kg dose by gastric gavage for ninety days. At the end of the study, TSA level was determined in serum, liver, kidney, brain and testicular tissues. Compared with the control group, it was found that TSA levels increased in serum, liver, kidney, brain and testis tissues in the group treated with sodium fluoride (p<0.05). According to the NaF group, there was a significant decrease in serum levels in the NaF+CH group and in the serum, liver and kidney tissues in the NaF+COS group. It was determined that there was no difference between the control and experimental groups in terms of brain tissue sialic acid level (p>0.05). In conclusion, it can be thought that fluorine intoxication causes an increase in TSA levels by causing cell damage in serum and tissues. In the study presented, CH and COS have been shown to reduce TSA levels. Also, in this study, COS was found to be more effective in reducing the TSA level.
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Primary Language en
Subjects Science, Biology
Published Date Haziran-2021
Journal Section Kimya / Chemistry, Kimya Mühendisliği / Chemical Engineering
Authors

Orcid: 0000-0002-0709-1545
Author: Ugur OZDEK (Primary Author)
Institution: Vocational School of Health Services, Van Yüzüncü Yıl University
Country: Turkey


Orcid: 0000-0002-9023-0699
Author: Yeter DEĞER
Institution: Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Biyokimya Anabilim Dalı, 65040, Van, Türkiye
Country: Turkey


Supporting Institution Van Yüzüncü Yıl University Scientific Research Projects Department
Project Number [TSA-2018-7404].
Dates

Application Date : November 27, 2020
Acceptance Date : February 25, 2021
Publication Date : June 1, 2021

Bibtex @research article { jist830307, journal = {Journal of the Institute of Science and Technology}, issn = {2146-0574}, eissn = {2536-4618}, address = {}, publisher = {Igdir University}, year = {2021}, volume = {11}, pages = {1402 - 1407}, doi = {10.21597/jist.830307}, title = {Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis}, key = {cite}, author = {Ozdek, Ugur and Değer, Yeter} }
APA Ozdek, U , Değer, Y . (2021). Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis . Journal of the Institute of Science and Technology , 11 (2) , 1402-1407 . DOI: 10.21597/jist.830307
MLA Ozdek, U , Değer, Y . "Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis" . Journal of the Institute of Science and Technology 11 (2021 ): 1402-1407 <https://dergipark.org.tr/en/pub/jist/issue/61423/830307>
Chicago Ozdek, U , Değer, Y . "Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis". Journal of the Institute of Science and Technology 11 (2021 ): 1402-1407
RIS TY - JOUR T1 - Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis AU - Ugur Ozdek , Yeter Değer Y1 - 2021 PY - 2021 N1 - doi: 10.21597/jist.830307 DO - 10.21597/jist.830307 T2 - Journal of the Institute of Science and Technology JF - Journal JO - JOR SP - 1402 EP - 1407 VL - 11 IS - 2 SN - 2146-0574-2536-4618 M3 - doi: 10.21597/jist.830307 UR - https://doi.org/10.21597/jist.830307 Y2 - 2021 ER -
EndNote %0 Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis %A Ugur Ozdek , Yeter Değer %T Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis %D 2021 %J Journal of the Institute of Science and Technology %P 2146-0574-2536-4618 %V 11 %N 2 %R doi: 10.21597/jist.830307 %U 10.21597/jist.830307
ISNAD Ozdek, Ugur , Değer, Yeter . "Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis". Journal of the Institute of Science and Technology 11 / 2 (June 2021): 1402-1407 . https://doi.org/10.21597/jist.830307
AMA Ozdek U , Değer Y . Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis. Iğdır Üniv. Fen Bil Enst. Der.. 2021; 11(2): 1402-1407.
Vancouver Ozdek U , Değer Y . Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis. Journal of the Institute of Science and Technology. 2021; 11(2): 1402-1407.
IEEE U. Ozdek and Y. Değer , "Effects of Chitosan and Chitosan Oligosaccharide on Serum and Tissue Sialic Acid Levels in Experimental Fluorosis", Journal of the Institute of Science and Technology, vol. 11, no. 2, pp. 1402-1407, Jun. 2021, doi:10.21597/jist.830307