Year 2021, Volume 11 , Issue 2, Pages 927 - 932 2021-06-01

Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)
Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)

İsmail BEZİRGANOGLU [1]


The goal of this study was to determine melatonin impacts on the embryogenic callus formation and callus necrosis recovering of alfalfa (Medicago sativa L). Calluses, obtained from leaf explants, were cultivated on Murashige and Skoog (MS) medium supplemented with 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D or 0.0125 mg mL-1 kinetin + 1 mg mL-1 melatonin. Melatonin reduced the embryogenic callus formation in both concentrations. It was detected that the weight of fresh callus in genotypes was strongly related with hormones under in vitro conditions and the lowest fresh callus weight was detected in presence of 0.0125 mg mL-1 kinetin and 1 mg mL-1 melatonin. Based on the callus necrosis recovery, the highest callus necrosis weight (0.843 g) were recorded in embryogenic calluses cultivated in 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D melatonin-supplemented medium, compared with the calluses activited with MS alone (0.587 g). In conclusion, it was detected in this study that in vitro melatonin could be used in callus necrosis recovered in tissue cultures of alfalfa.
The goal of this study was to determine melatonin impacts on the embryogenic callus formation and callus necrosis recovering of alfalfa (Medicago sativa L). Calluses, obtained from leaf explants, were cultivated on Murashige and Skoog (MS) medium supplemented with 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D or 0.0125 mg mL-1 kinetin + 1 mg mL-1 melatonin. Melatonin reduced the embryogenic callus formation in both concentrations. It was detected that the weight of fresh callus in genotypes was strongly related with hormones under in vitro conditions and the lowest fresh callus weight was detected in presence of 0.0125 mg mL-1 kinetin and 1 mg mL-1 melatonin. Based on the callus necrosis recovery, the highest callus necrosis weight (0.843 g) were recorded in embryogenic calluses cultivated in 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D melatonin-supplemented medium, compared with the calluses activited with MS alone (0.587 g). In conclusion, it was detected in this study that in vitro melatonin could be used in callus necrosis recovered in tissue cultures of alfalfa.
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Primary Language en
Subjects Biology
Published Date Haziran-2021
Journal Section Biyoloji / Biology
Authors

Orcid: 0000-0003-4079-5998
Author: İsmail BEZİRGANOGLU (Primary Author)
Institution: ERZURUM TEKNİK ÜNİVERSİTESİ
Country: Turkey


Dates

Application Date : December 31, 2020
Acceptance Date : February 17, 2021
Publication Date : June 1, 2021

Bibtex @research article { jist851610, journal = {Journal of the Institute of Science and Technology}, issn = {2146-0574}, eissn = {2536-4618}, address = {}, publisher = {Igdir University}, year = {2021}, volume = {11}, pages = {927 - 932}, doi = {10.21597/jist.851610}, title = {Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)}, key = {cite}, author = {Bezirganoglu, İsmail} }
APA Bezirganoglu, İ . (2021). Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.) . Journal of the Institute of Science and Technology , 11 (2) , 927-932 . DOI: 10.21597/jist.851610
MLA Bezirganoglu, İ . "Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)" . Journal of the Institute of Science and Technology 11 (2021 ): 927-932 <https://dergipark.org.tr/en/pub/jist/issue/61423/851610>
Chicago Bezirganoglu, İ . "Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)". Journal of the Institute of Science and Technology 11 (2021 ): 927-932
RIS TY - JOUR T1 - Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.) AU - İsmail Bezirganoglu Y1 - 2021 PY - 2021 N1 - doi: 10.21597/jist.851610 DO - 10.21597/jist.851610 T2 - Journal of the Institute of Science and Technology JF - Journal JO - JOR SP - 927 EP - 932 VL - 11 IS - 2 SN - 2146-0574-2536-4618 M3 - doi: 10.21597/jist.851610 UR - https://doi.org/10.21597/jist.851610 Y2 - 2021 ER -
EndNote %0 Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.) %A İsmail Bezirganoglu %T Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.) %D 2021 %J Journal of the Institute of Science and Technology %P 2146-0574-2536-4618 %V 11 %N 2 %R doi: 10.21597/jist.851610 %U 10.21597/jist.851610
ISNAD Bezirganoglu, İsmail . "Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)". Journal of the Institute of Science and Technology 11 / 2 (June 2021): 927-932 . https://doi.org/10.21597/jist.851610
AMA Bezirganoglu İ . Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.). Iğdır Üniv. Fen Bil Enst. Der.. 2021; 11(2): 927-932.
Vancouver Bezirganoglu İ . Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.). Journal of the Institute of Science and Technology. 2021; 11(2): 927-932.
IEEE İ. Bezirganoglu , "Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)", Journal of the Institute of Science and Technology, vol. 11, no. 2, pp. 927-932, Jun. 2021, doi:10.21597/jist.851610