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Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi

Year 2021, Volume: 11 Issue: özel sayı, 3496 - 3506, 30.12.2021
https://doi.org/10.21597/jist.1029010

Abstract

Günümüzde tüketici bilincinin artması sonucu sağlıklı gıda ürünlerine talep her geçen gün artmaktadır.
Kimyasal bileşimi ve beslenme özelliklere sahip olan Tef bitkisi, insan beslenmesinde geçmişten beri kullanılan
bir bitki olmakla beraber, ülkemizde de alternatif olarak yetiştirilebilecek kaynaklardan biridir. Sağlıklı yaşam
açısından son derece önemli bir tahıl olan tef, glutensiz olması nedeniyle de özellikle çölyak hastalarının
beslenmesinde önemli yer tutar. Bunun yanında yetişme koşullarının zor olmaması ve hasat süresinin kısa
olmasıyla beraber gelecekte kuraklığa kolayca uyum sağlayabileceği düşüncesi tef tahılının değerini her geçen
gün arttırmaktadır. Çalışma kapsamında Et 2016 tef bitkisi kullanılarak 19 RAPD markırı ile taranmıştır. İyi
amplikon veren 10 tane RAPD primeri ile Tef bitkisinde genetik varyasyon araştırması yapılmıştır. Kullanılan 10
tane RAPD primeri ile PZR sonrası agaroz jel elektroforez yöntemi ile amplikonların ayrıştırılması sağlanarak
varlık (1) ve yokluk (0) skorlaması gerçekleştirilmiştir. Elde edilen verilere ile Jaccard benzerlik indeksi
kullanılarak temel koordinat analizi ve Bayesian istatistiği ile genotipler arasında dendogram oluşturulmuştur.
Yapılan analizler sonucunda genotipler arasındaki genetik varyasyonun düşük olduğu saptanmıştır. Zobel Basic
ve Dega Basic genotiplerinde çok az düzeyde farklılık tespit edilmiş olup, daha çok markır ile teyit edilmesi
gerekmektedir. Sonuç olarak bu genotipler arasındaki varyasyonun artırılması için farklı yaklaşımlar kullanılarak
(mutajenler vb.) varyasyonun artırılması önerilmektedir.

Thanks

Çalışma materyalini bize gönderdiği için Etiyopya Wolla Üniversitesinden Dr. Seid Hussen Muhie’ye teşekkür ederiz.

References

  • Abdellatif KF, Soliman YA, 2013. Genetic Relationships of Cotton (Gossypium barbadense L.) Genotypes as Studied by Morphological and Molecular Markers. African Journal of Biotechnology, 12: 4736-4746.
  • Assefa K, Chanyalew S, Tadele Z, 2017. “Tef Eragrostis tef (Zucc.) Trotter Millets and Sorghum,” in Biology and Genetic Improvement, J. V. Patil, Ed., 2017 John Wiley & Sons Ltd. Published by John Wiley & Sons Ltd, First edition.
  • Assefa K, Yu JK, Zeid M, Belay G, Tefera H, Sorrells ME, 2011. Breeding tef [Eragrostis tef (Zucc.) trotter]: conventional and molecular approaches. Plant Breeding, doi:10.1111/j.1439-0523.2010.01782.x
  • Aydın, 2018.Türkiye’de Tescillenmiş Bazı Ticari Pamuk Çeşitlerinin Moleküler Karakterizasyonu Üzerine Bir Araştırma. Akdeniz Üniversitesi Doktora Tezi.
  • Aydin A, Ince AG, Uygur Gocer E, Karaca M, 2018. Single Cotton Seed DNA Extraction without the Use of Enzymes and Liquid Nitrogen. Fresenius Environmental Bulletin, 27: 6722-6726.
  • Conert HJ, 1992. Eragrostoideae. in Hegi G (Ed.) Illustriert Flora von Mittel-Europa. Band. I, Vol. 3. Spermatophyta: Angiospermae: Monocotyledones 1(2) Poaceae (Parey Buchverlag: Berlin). Pp. 75–120.
  • Eckhoff JLA, Wichman DM, Scheetz J, Majerus M, Welty LE, Stallknecht GF, Ditterline RL, Dunn RL, Sands DC, 1993. Teff: a potential forage and grain crop for Montana. Montana AgResearch, 10:38–41.
  • Gebremariam MM, Zarnkow M, Becker T, 2014. Teff (Eragrostis tef) as a raw material for malting, brewing and manufacturing of gluten-free foods and beverages: a review, Journal of Food Science and Technology, 51(11):2881–2895.
  • Geren H, Kavut YT, Kır B, 2019. Effect of Different Row Spacings on the Yield and Some Yield Characteristics of Teff (Eragrostis tef) Crop Grown under Söke Ecological Conditions, Ege Üniversitesi Ziraat Fakültesi Dergisi, 56 (2):231-239.
  • Govindaraj M, Vetriventhan M, Srinivasan M, 2014, Importance of Genetic Diversity Assessment in Crop Plants and Its Recent Advances: An Overview of Its Analytical Perspectives Genetics Research International, 31487.
  • Hickman AL, Abaye OA, McCann MA, McCann JS, 2013. Acceptability and nutritional value of the teff grass for grazing horses, Journal of Equine Veterinary Science, 33:321-399.
  • Ince AG, Karaca M, 2009. The MAGi RNA extraction method: a highly efficient and simple procedure for fresh and dry plant tissues. Journal of the Science of Food and Agriculture, 89: 168-176.
  • İnal B, 2011. Bitki Moleküler Filogenisinde Kullanılan Bazı Gen ve Metodların Karşılaştırılması. Akdeniz Üniversitesi Yüksek Lisans Tezi.
  • Karaca M, Aydin A, Ince, AG, 2019. Cytosine methylation polymorphisms in cotton using TD-MS-RAPD-PCR. Modern Phytomorphology, 13: 13-19.
  • Kaya C, Tiryaki I, Sari U, Tuna M, 2020. Genetic relationship and nuclear dna content variation in Tef [Eragrostis tef (Zucc.) Trotter] accessions. Molecular Biology Reports, 47: 4455-4463.
  • Köten M, 2021. Development of tef [Eragrostis tef (Zucc.) Trotter] based gluten‐free tarhana. Journal of Food Processing and Preservation, 45(1), e15133.
  • Lukonge E, Herselman L, Labuschangne MT, 2007. Genetic Diversity of Tanzanian Cotton (Gossypium hirsutum L.) revealed by AFLP analysis. African Journal Crop Science, 8: 773-776.
  • Mebratu Y, Raghavaiah CV, Ashagre H, 2016. Production potential of tef (Eragrostis tef (Zucc.) Trotter) genotypes in relation to integrated nutrient management on vertisols of mid high lands of Oromia Region of Ethiopia, East Africa. Adv. Crop Sci. Tech. 4:249.
  • Rana MK, Bhat KV, 2005. RAPD Marker for Genetic Diversity Study Among Indian Cotton Cultivars. Current Science, 88, 1956-1961.
  • Sarı U, Tiryaki İ, 2018. Alternatif Tahıl: Eskinin Unutulmuş Yeni Bitkisi Tef (Eragrostis tef [Zucc.] Trotter). KSÜ Tarim ve Doğa Derg 21(3). 447-456. DOI:10.18016/ ksudobil.328540
  • Smith JSC, Chin ECL, Shu H, Smith OS, Wall SJ, Senior ML, Mitchell SE, Kresovich S, Ziegle J, 1997. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theoretical and Applied Genetics 95: 163-173.
  • Tadesse M, Kebede M, Girma D, 2021. Genetic Diversity of Tef [Eragrostis tef (Zucc.)Trotter] as Revealed by Microsatellite Markers. https://doi.org/10.1155/2021/6672397.
  • Tyagi P, Gore MA, Bowman DT, Campbell BT, Udall JA, Kuraparthy V, 2014. Genetic Diversity and Population Structure in the US Upland Cotton (Gossypium hirsutum L.). Theoretical Applied Genetics, 127: 283-295.
  • Ulukan H, 2011. The use of plant genetic resources and biodiversity in classical plant breeding. Acta Agriculturae Scandinavica, Section B - Plant Soil Science, 61 (2), 97-104.
  • Van Esbroeck GA, Bowman DT, May OL, Calhoun DS, 1999. Genetic Similarity Indices for Ancestral Cotton Cultivars and Their Impact on Genetic Diversity Estimates of Modern Cultivars. Crop Science, 39: 323-328.
  • Wendel JF, Brubaker CL, Percival AE, 1992. Genetic Diversity in Gossypium hirsutum L. and the Origin of Upland cotton. American Journal of Botany, 79: 1291-1310.
  • Yu JZ, Fang DD, Kohel RJ, Ulloa M, Hinze LL, Percy RG, Zhang J, Chee P, Scheffler BE, Jones DC, 2012. Development of a Core Set of SSR Markers for the Characterization of Gossypium Germplasm. Euphytica, 187: 203-213.

Determination of Genetic Variation in Some Teff Genotypes Using RAPD Markers

Year 2021, Volume: 11 Issue: özel sayı, 3496 - 3506, 30.12.2021
https://doi.org/10.21597/jist.1029010

Abstract

As a result of increasing consumer awareness, the demand for healthy food products is increasing
day by day. Teff, which has chemical composition and nutritional properties, is a plant that has been used in
human nutrition since the past, and it is one of the sources that can be grown as an alternative in our country.
Being a very important grain for a healthy life, teff has an important place in the diet of celiac patients because it
is gluten-free. Besides, the fact that the growing conditions are not difficult and the harvest period is short, the
thought that it can easily adapt to drought in the future increases the value of teff grain day by day. Within the
scope of the study, 19 RAPD markers were screened using Et 2016 genotip. Genetic variation research was
conducted in Teff plant with 10 RAPD primers giving good amplicon. Presence (1) and absence (0) scoring were
performed by separating amplicons using agarose gel electrophoresis method after PCR with 10 RAPD primers
used. By using the Jaccard similarity index with the data obtained, a dendogram was created between the
genotypes with the basic coordinate analysis and Bayesian statistics. As a result of the analysis, it was determined
that the genetic variation between the genotypes was low. Very little difference was detected in Zobel Basic and
Dega Basic genotypes and needs to be confirmed with more markers. As a result, it is recommended to increase
the variation by using different approaches (mutagens, UV, etc.) to increase the variation among these genotypes.

References

  • Abdellatif KF, Soliman YA, 2013. Genetic Relationships of Cotton (Gossypium barbadense L.) Genotypes as Studied by Morphological and Molecular Markers. African Journal of Biotechnology, 12: 4736-4746.
  • Assefa K, Chanyalew S, Tadele Z, 2017. “Tef Eragrostis tef (Zucc.) Trotter Millets and Sorghum,” in Biology and Genetic Improvement, J. V. Patil, Ed., 2017 John Wiley & Sons Ltd. Published by John Wiley & Sons Ltd, First edition.
  • Assefa K, Yu JK, Zeid M, Belay G, Tefera H, Sorrells ME, 2011. Breeding tef [Eragrostis tef (Zucc.) trotter]: conventional and molecular approaches. Plant Breeding, doi:10.1111/j.1439-0523.2010.01782.x
  • Aydın, 2018.Türkiye’de Tescillenmiş Bazı Ticari Pamuk Çeşitlerinin Moleküler Karakterizasyonu Üzerine Bir Araştırma. Akdeniz Üniversitesi Doktora Tezi.
  • Aydin A, Ince AG, Uygur Gocer E, Karaca M, 2018. Single Cotton Seed DNA Extraction without the Use of Enzymes and Liquid Nitrogen. Fresenius Environmental Bulletin, 27: 6722-6726.
  • Conert HJ, 1992. Eragrostoideae. in Hegi G (Ed.) Illustriert Flora von Mittel-Europa. Band. I, Vol. 3. Spermatophyta: Angiospermae: Monocotyledones 1(2) Poaceae (Parey Buchverlag: Berlin). Pp. 75–120.
  • Eckhoff JLA, Wichman DM, Scheetz J, Majerus M, Welty LE, Stallknecht GF, Ditterline RL, Dunn RL, Sands DC, 1993. Teff: a potential forage and grain crop for Montana. Montana AgResearch, 10:38–41.
  • Gebremariam MM, Zarnkow M, Becker T, 2014. Teff (Eragrostis tef) as a raw material for malting, brewing and manufacturing of gluten-free foods and beverages: a review, Journal of Food Science and Technology, 51(11):2881–2895.
  • Geren H, Kavut YT, Kır B, 2019. Effect of Different Row Spacings on the Yield and Some Yield Characteristics of Teff (Eragrostis tef) Crop Grown under Söke Ecological Conditions, Ege Üniversitesi Ziraat Fakültesi Dergisi, 56 (2):231-239.
  • Govindaraj M, Vetriventhan M, Srinivasan M, 2014, Importance of Genetic Diversity Assessment in Crop Plants and Its Recent Advances: An Overview of Its Analytical Perspectives Genetics Research International, 31487.
  • Hickman AL, Abaye OA, McCann MA, McCann JS, 2013. Acceptability and nutritional value of the teff grass for grazing horses, Journal of Equine Veterinary Science, 33:321-399.
  • Ince AG, Karaca M, 2009. The MAGi RNA extraction method: a highly efficient and simple procedure for fresh and dry plant tissues. Journal of the Science of Food and Agriculture, 89: 168-176.
  • İnal B, 2011. Bitki Moleküler Filogenisinde Kullanılan Bazı Gen ve Metodların Karşılaştırılması. Akdeniz Üniversitesi Yüksek Lisans Tezi.
  • Karaca M, Aydin A, Ince, AG, 2019. Cytosine methylation polymorphisms in cotton using TD-MS-RAPD-PCR. Modern Phytomorphology, 13: 13-19.
  • Kaya C, Tiryaki I, Sari U, Tuna M, 2020. Genetic relationship and nuclear dna content variation in Tef [Eragrostis tef (Zucc.) Trotter] accessions. Molecular Biology Reports, 47: 4455-4463.
  • Köten M, 2021. Development of tef [Eragrostis tef (Zucc.) Trotter] based gluten‐free tarhana. Journal of Food Processing and Preservation, 45(1), e15133.
  • Lukonge E, Herselman L, Labuschangne MT, 2007. Genetic Diversity of Tanzanian Cotton (Gossypium hirsutum L.) revealed by AFLP analysis. African Journal Crop Science, 8: 773-776.
  • Mebratu Y, Raghavaiah CV, Ashagre H, 2016. Production potential of tef (Eragrostis tef (Zucc.) Trotter) genotypes in relation to integrated nutrient management on vertisols of mid high lands of Oromia Region of Ethiopia, East Africa. Adv. Crop Sci. Tech. 4:249.
  • Rana MK, Bhat KV, 2005. RAPD Marker for Genetic Diversity Study Among Indian Cotton Cultivars. Current Science, 88, 1956-1961.
  • Sarı U, Tiryaki İ, 2018. Alternatif Tahıl: Eskinin Unutulmuş Yeni Bitkisi Tef (Eragrostis tef [Zucc.] Trotter). KSÜ Tarim ve Doğa Derg 21(3). 447-456. DOI:10.18016/ ksudobil.328540
  • Smith JSC, Chin ECL, Shu H, Smith OS, Wall SJ, Senior ML, Mitchell SE, Kresovich S, Ziegle J, 1997. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theoretical and Applied Genetics 95: 163-173.
  • Tadesse M, Kebede M, Girma D, 2021. Genetic Diversity of Tef [Eragrostis tef (Zucc.)Trotter] as Revealed by Microsatellite Markers. https://doi.org/10.1155/2021/6672397.
  • Tyagi P, Gore MA, Bowman DT, Campbell BT, Udall JA, Kuraparthy V, 2014. Genetic Diversity and Population Structure in the US Upland Cotton (Gossypium hirsutum L.). Theoretical Applied Genetics, 127: 283-295.
  • Ulukan H, 2011. The use of plant genetic resources and biodiversity in classical plant breeding. Acta Agriculturae Scandinavica, Section B - Plant Soil Science, 61 (2), 97-104.
  • Van Esbroeck GA, Bowman DT, May OL, Calhoun DS, 1999. Genetic Similarity Indices for Ancestral Cotton Cultivars and Their Impact on Genetic Diversity Estimates of Modern Cultivars. Crop Science, 39: 323-328.
  • Wendel JF, Brubaker CL, Percival AE, 1992. Genetic Diversity in Gossypium hirsutum L. and the Origin of Upland cotton. American Journal of Botany, 79: 1291-1310.
  • Yu JZ, Fang DD, Kohel RJ, Ulloa M, Hinze LL, Percy RG, Zhang J, Chee P, Scheffler BE, Jones DC, 2012. Development of a Core Set of SSR Markers for the Characterization of Gossypium Germplasm. Euphytica, 187: 203-213.
There are 27 citations in total.

Details

Primary Language Turkish
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Moleküler Biyoloji ve Genetik / Moleculer Biology and Genetic
Authors

Adnan Aydın 0000-0002-8284-3751

Eren Özden 0000-0001-7507-9815

Early Pub Date December 29, 2021
Publication Date December 30, 2021
Submission Date November 27, 2021
Acceptance Date December 7, 2021
Published in Issue Year 2021 Volume: 11 Issue: özel sayı

Cite

APA Aydın, A., & Özden, E. (2021). Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi. Journal of the Institute of Science and Technology, 11(özel sayı), 3496-3506. https://doi.org/10.21597/jist.1029010
AMA Aydın A, Özden E. Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi. J. Inst. Sci. and Tech. December 2021;11(özel sayı):3496-3506. doi:10.21597/jist.1029010
Chicago Aydın, Adnan, and Eren Özden. “Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi”. Journal of the Institute of Science and Technology 11, no. özel sayı (December 2021): 3496-3506. https://doi.org/10.21597/jist.1029010.
EndNote Aydın A, Özden E (December 1, 2021) Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi. Journal of the Institute of Science and Technology 11 özel sayı 3496–3506.
IEEE A. Aydın and E. Özden, “Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi”, J. Inst. Sci. and Tech., vol. 11, no. özel sayı, pp. 3496–3506, 2021, doi: 10.21597/jist.1029010.
ISNAD Aydın, Adnan - Özden, Eren. “Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi”. Journal of the Institute of Science and Technology 11/özel sayı (December 2021), 3496-3506. https://doi.org/10.21597/jist.1029010.
JAMA Aydın A, Özden E. Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi. J. Inst. Sci. and Tech. 2021;11:3496–3506.
MLA Aydın, Adnan and Eren Özden. “Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi”. Journal of the Institute of Science and Technology, vol. 11, no. özel sayı, 2021, pp. 3496-0, doi:10.21597/jist.1029010.
Vancouver Aydın A, Özden E. Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi. J. Inst. Sci. and Tech. 2021;11(özel sayı):3496-50.