Streptomyces sp. K22 ve K30 Suşlarından Lipaz ve Proteaz Enzim Üretimi

Year 2017, , 128 - 135, 15.12.2017

Kadriye Özcan , Cengiz Çorbacı

https://doi.org/10.31466/kfbd.343293

Cited By: 4

Abstract

Mikrobiyal enzimler önemli bir biyoteknolojik
araçlardır. Birçok biyolojik ve kimyasal reaksiyonda rol almaktadırlar ve
çeşitli endüstrilerdeki farklı alanlarda kendilerine önemli sayılabilecek bir
yer edinmişlerdir. Mikrobiyal enzimler arasından lipaz ve proteazlar, bu grubun
önemli birer üyeleridirler. Dolayısıyla, bu enzimleri üreten
mikroorganizmaların seçimi ve ürettikleri enzimlerin karakteristiklerinin
saptanması, pek çok araştırmacıyı bu alanda çalışmaya teşvik etmektedir. Bu
çalışmada, Karadeniz deniz sediment örneklerinden izole edilmiş aktinobakteri
suşlarının hücre dışı lipaz ve proteaz enzim üretim kapasitelerinin
belirlenmesi ve pH ile sıcaklık kararlılıklarının saptanması amaçlanmıştır.
Çalışmadan elde edilen sonuçlar değerlendirildiğinde, bu organizmaların iyi
birer lipaz ve proteaz enzim üreticisi ve bunların enzimlerinin geniş birer pH
ve sıcaklık kararlılıklarına sahip oldukları saptanmıştır

Keywords

Lipaz, proteaz, stabilite, Streptomyces sp.

Production of Lipase and Protease Enzymes from Streptomyces sp. K22 and K30 Strains

Abstract

Microbial enzymes are important biotechnological
tools. They play role in several biological and chemical reactions and have
acquired a significant place in different fields in various industries. Among
microbial enzymes, lipases and proteases are important members of the group.
Therefore, the selection of microorganisms producing these enzymes and the
determination of their characteristics trigger researchers to work on this
field. In this study, it was aimed to investigate production capacities of
extracellular lipase and protease enzymes by actinobacteria strains isolated
from Black Sea sediment samples and to determine their pH and temperature
stabilities. In lights of the results obtained from the study, it was found that
these organisms are well-producer of lipase and protease enzymes, and their
enzymes have a wide range of pH and temperature stability.

Keywords

Lipase, protease, stability, Streptomyces sp.

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