Electron Paramagnetic Resonance (EPR) Study of Gamma-Irradiated Glycinamide Hydrochloride Powder Crystals

Öz

In this study, free radical structures formed in gamma-irradiated Glycinamide Hydrochloride (GAHCl) powder crystals were investigated using Electron Paramagnetic Resonance (EPR) spectroscopy. EPR spectra of the sample irradiated with a dose of 20 kGy were recorded, and the H₂NĊHCONH₂ radical, presumed to have formed as a result of irradiation, was identified. The obtained EPR parameters (g-value: 2.0036, line width: 0.90 mT, hyperfine coupling constants: aCH = 1.23 mT, aN = 0.48 mT) were consistent with the simulation data and matched the values reported for similar glycine derivatives in the literature. Additionally, the chemical and biological properties of the sample were comprehensively investigated. These findings provide significant insights into the structural changes induced by gamma irradiation in amino acid derivatives and demonstrate the potential of Glycinamide Hydrochloride as a model compound in biological and pharmaceutical research.

Anahtar Kelimeler

• Amino Acid Derivative
• Electron Paramagnetic Resonance
• Free Radicals.

Gama ile Işınlanmış Glisinamid Hidroklorür Toz Kristallerinin Elektron Paramanyetik Rezonans (EPR) İncelenmesi

Öz

Bu çalışmada, gama ışınları ile ışınlanmış Glisinamid Hidroklorür (GAHCl) toz kristallerinde oluşan serbest radikal yapılar, Elektron Paramanyetik Rezonans (EPR) spektroskopisi kullanılarak incelenmiştir. 20 kGy dozda ışınlanan örneğin EPR spektrumları kaydedilmiş ve ışınlama sonucu oluştuğu düşünülen H₂NĊHCONH₂ radikali tespit edilmiştir. Elde edilen EPR parametreleri (g-değeri: 2.0036, çizgi genişliği: 0.90 mT, aşırı ince yapı sabitleri: aCH = 1.23 mT, aN = 0.48 mT), simülasyon verileriyle uyumlu olup literatürdeki benzer glisin türevleriyle karşılaştırıldığında tutarlıdır. Ayrıca örneğin kimyasal ve biyolojik özellikleri kapsamlı bir şekilde araştırılmıştır. Bu sonuçlar, gama ışınlarının amino asit türevlerinde oluşturduğu yapısal değişikliklerin anlaşılması açısından önemli veriler sunmakta ve Glisinamid Hidroklorür’ün model bileşik olarak biyolojik ve farmasötik araştırmalarda kullanılabilirliğini göstermektedir.

Anahtar Kelimeler

• Amino Asit Türevi
• Elektron Paramanyetik Rezonans
• Serbest Radikal

Kaynakça

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