Characterization, Identification and Phylogeny of the Creatine Kinase (ckma) Gene in Medaka (Oryzias latipes)

Abstract

Creatine kinase (ckma) has been characterized and described in the medaka (Oryzias latipes), an aquatic model organism and the gene structure has been designed using the exons, introns, produced amino acids of the gene, TATA box, poly A tail and 5’ UTR and 3’ UTR regions of the ckma gene. In another step, firstly, the chromosome region of the ckma gene was determined in medaka and then the other genes which placed in the same region were determined. Then the locations of these genes were determined in zebrafish and human which are the orthologs of medaka. Finally, the conserved gene synteny was designed manually, using these data. However, genetic identity and similarity ratio between medaka and its orthologs were calculated. In this study, characterization and identification, phylogenetic relationship, conserved gene synteny of ckma gene in medaka (O. latipes) which is an important model organism were analyzed by using bioinformatics tools (NCBI database, Ensembl genomic database, Expasy, Reverse Complementary and some programs such as MEGA6 program, BLOSUM62 matrix program and BioEdit software). All these data will be used in future studies on molecular stress response in fish and they were presented to the scientific world with this study.

Keywords

• Medaka
• Genomic organization
• Model organism
• Bioinformatics

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