Detection of complex vertebral malformation in holstein cattle reared in Antalya using allele-spesific PCR

Year 2019, Volume: 32 Issue: 3, 443 - 446, 01.12.2019

Murat Gökçe Eren Murat Soner Balcıoğlu , Eymen Demir

https://doi.org/10.29136/mediterranean.578015

Cited By: 3

https://izlik.org/JA89HZ79WE

Abstract

This
study aimed to analyze complex vertebral malformation (CVM) genetic defect in
Holstein raised in Antalya province using allele specific PCR (AS-PCR). The
presence of CVM mutant allele was investigated in 200 Holstein cows raised by
member breeders of Antalya Cattle Breeders Association and raised in Akdeniz
University, Faculty of Agriculture. Total genomic
DNA was extracted from blood samples obtained from Holstein cows. In the 395 bp
length of SLC35A3 gene region of
bovine chromosome 3, different primers were used to
identify the normal and mutant allele. By using agarose gel electrophoresis,
individuals were genotyped after AS-PCR. In this study, 7 of 200 individuals
were found as CVM carriers.

Keywords

CVM , SLC35A3 gene , Hereditary diseases

Project Number

2014.02.0121.016

Antalya’da yetiştirilen holstein sığırlarında kompleks vertebral malformasyon kalıtsal hastalığının allele özgü PCR ile belirlenmesi

https://izlik.org/JA89HZ79WE

Abstract

Bu çalışmada kalıtsal bir hastalık olan kompleks vertebral
malformasyonun (CVM) Antalya ilinde yetiştiriciliği yapılan Siyah Alaca
sığırlarında varlığı allele özgü polimeraz zincir reaksiyonu (AS-PCR)
kullanılarak araştırılmıştır. Antalya Damızlık Sığır Yetiştiricileri Birliğine
üye işletmelerden ve aynı birliğe üye olan Akdeniz Üniversitesi, Ziraat
Fakültesi süt sığırcılığı işletmesine ait toplam 200 bireyde CVM kalıtsal
hastalığının varlığı araştırılmıştır. Holstein sığırlarından elde edilen kan
örneklerinden total genomik DNA izole
edilmiştir. Sığır genomunun 3. kromozomu üzerinde
bulunan SLC35A3 geninin 395 bç
uzunluğundaki bölgesinde normal ve mutant allellerin belirlenmesinde farklı
primerler kullanılmıştır. AS-PCR işleminden sonra uygulanan agaroz jel
elektroforezi sonuçlarına göre bireyler genotiplendirilmiştir. Bu çalışmada;
incelenen 200 bireyden 7 tanesinin CVM taşıyıcısı olduğu belirlenmiştir.

Keywords

CVM , SLC35A3 geni , Kalıtsal hastalıklar

Supporting Institution

Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

2014.02.0121.016

Thanks

Bu çalışma Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 2014.02.0121.016 nolu proje ile desteklenmiştir.

References

• Adamov N, Mitrov D, Esmerov I, Dovc P (2014) Detection of recessive mutations (BLAD and CVM) in Holstein-Friesian cattle population in Republic of Macedonia, Macedonian Veterinary Review 37: 61-68.
• Agerholm JS, Bendixen C, Andersen O, Arnbjerg J (2001) Complex vertebral malformation in Holstein calves, Journal of Veterinary Diagnostic Investigation 16: 283-289.
• Agerholm JS, Bendixen C, Arnbjerg J, Andersen O (2004) Morphological variation of ‘complex vertebral malformation’ in Holstein calves Journal of Veterinary Diagnostic Investigation 16: 548-553.
• Avanus K, Altınel A (2017a) Comparison of allele-spesific PCR, created restriction-site PCR, and PCR with primer-introduced restriction analysis methods used for screening complex vertebral malformation carriers in Holstein cattle. Journal of Veterinary Science 18: 465-470.
• Avanus K, Altınel A (2017b) Inherited diseases of Holstein cattle: Stroy so far in Turkey. Journal of Istanbul Veterinary Sciences 1: 40-46.
• Berglund B, Persson A, Stalhammar H (2004) Effects of complex vertebral malformation on fertility in Swedish Holstein cattle. Acta Veterinaria Scandinavica 45: 161- 165.
• Chu Q, Sun D, Yu Y, Zhang Y, Zhang Y (2008) Identification of complex vertebral malformation carriers in Chinese Holstein. Journal of Veterinary Diagnostic Investigation 20: 228-230.
• Citek J, Rehout V, Hajkova J, Pavkova J (2006) Monitoring of the genetic health of cattle in the Czech Republic. Veterinarni Medicina 51: 333-339.
• Duncan RB, Jr, Carrig CB, Agerholm JS, Bendexian C (2001) Complex vertebral malformation in a Holstein calf: Report of a case in the USA. Journal of Veterinary Diagnostic Investigation 13: 283-289.
• Gabor M, Miluchova M, Trakovicka A, Riecka Z, Candrak J, Vavrisinova K (2012) Detection of complex vertebral malformation carriers in Slovak Haolstein cattle by high resolution melting analysis. Acta Veterinaria 62: 239-248.
• Ghanem ME, Akita M, Suzuki T, Kasuga A, Nishibori M (2008) Complex vertebral malformation in Holstein cows in Japan and its inheritance to crossbred F1 generation. Animal Reproduction Science 103: 348-354.
• Hemati B, Gharaie-Fathabad S, Fazeli MH, Namvar Z, Ranji M (2015) Investigation of bovine leukocyte adhesion deficiency (BLAD) and complex vertebral malformation (CVM) in apopulation of Iranian Holstein cows. Iranian Journal of Apllied Animal Science 5: 69-72.
• Kanae Y, Endoh D, Nagahata H, Hayashi M (2005) A method for detection complex vertebral vertebral malformation in Holstein calves using polymerase chain reaction-primer introduced restriction analysis. Journal of Veterinary Diagnostic Investigation 17: 258-262.
• Kociba GD, Ratnoff OD, Loeb WF, Wall RL, Heider LE (1969) Bovine thromboplastin antecedent (factor XI) deficiency. Journal of Laboratory and Clinical Medicine 74: 37-41.
• Meydan H, Yildiz MA, Agerholm JS (2010) Screening for bovine leukocyte adhesion deficiency, deficiency of uridine monophoshate synthase, complex vertebral malformation, bovine citrullinaemia, and factor XI deficiency in Holstein cows reared in Turkey. Acta Veterinaria Scandinavica 52: 56.
• Miller S, Dykes D, Plesky HA (1988) Simple Salting out Procedure for Extracting DNA from Human Cells. Nucleic Acids Research 16: 1215.
• Nagahata H, Oota H, Nitanai A, Oikawa S, Higuchi H, Nakade T, Kurosawa T, Morita M, Ogawa H (2002) Complex vertebral malformation in a stillborn Holstein calf in Japan. Journal of Veterinary Medical Science 64: 1107-1112.
• UNITED NATIONS (2019) World population 2019: World chart. https://population.un.org/wpp/DataQuery/. Accessed 1 June 2019.
• Rezaee AR, Nassiry MR, Valizadeh R, Tahmoorespour M, Javadmanesh A, Zarei A, Janati H (2008) Study of complex vertebral malformation disorder in Iranian Holstein Bulls. World Journal of Zoology 3: 36-39.
• Robinson JL, Dombrowski DB, Harpestad GW, Shanks RD (1984) Detection and prevalance of UMP synthase deficiency among dairy cattle. Journal of Heredity 75: 277-280.
• Rusc A, Kaminski S (2007) Prevalence ofcomplex vertebral malformation carriersamong Polish Holstein-Friesian bulls. Journal of Applied Genetics 48: 247-252.
• Rusc A, Hering D, Puckowska P, Barcewicz M, Kaminski S (2013) Screening of Polish Holstein-Friesian bulls towards eradication of complex vertebral malformation (CVM) carriers. Polish Journal of Veterinary Sciences 16: 579-581.
• Schütz E, Scharfenstein M, Brenig B (2008) Implication of complex vertebral malformation and bovine leukocyteadhesion deficiency DNA-based testing on disease frequency in the Holstein population. Journal of Dairy Science 91: 4854-4859.
• Shuster DE, Kehrli ME, Ackermann MR, Gilbert RO (1992) Identification and prevalance of a genetic defect that causes leukocyte adhesion deficiency in Holstein cattle. Proceedings of the National Academy of Sciences of the United States of America 89: 9225-9229.
• Wang C, Tong Q, Hu XZ, Yang LG, Zhong XO, Yu Y, Wu JJ, Liu WJ, Li X, Hua GH, Zhao HQ, Zhang SJ (2011) Identification of complex vertebral malformation carriers in Holstein cattle in South China. Genetics and Molecular Research 10: 2443-2448.
• Thomsen B, Horn P, Panitz F, Bendixen E, Petersen A H, Holm, LE, Nielsen VH, Agerholm JS, Arnbjerg J, Bendixen C (2006) A missense mutation in the bovine SLC35A3 gene, encoding a UDP-Nacetylglucosamine transporter, causes complex vertebral malformation. Genome Reserch 16: 97–105.
• Zhang Y, Fan X, Sun D, Wang Y, Yu Y, Xie Y, Zhang S, Zhang Y (2012) A novel method for rapid and reliable detection of complex vertebral malformation and bovine leukocyte adhesion deficiency in Holstein cattle. Journal of Animal Science and Biotechnology 3: 24.