Determination of essential oil content and its chemical compositions of pink rock rose (Cistus creticus L.) collected from different altitudes

Year 2022, Volume: 37 Issue: 2, 263 - 274, 30.06.2022

Burhan Arslan Emrullah Culpan

https://doi.org/10.7161/omuanajas.957661

Abstract

This research was conducted in 2019 to determine the essential oil content and chemical composition of pink rock rose (Cistus creticus L.) which is spread over a wide area in Kumbağ, Yeniköy and Uçmakdere of Tekirdağ. For this purpose, the leaves of the Cistus creticus L. plant were collected from different altitudes (0, 50, 100 and 150 m) and dried at room temperature, and essential oil content was determined in the samples with the help of Clevenger Apparatus. Chemical composition of the essential oil was examined with GC/MS. According to the research results, 13 components representing 73.03% (≥1%) of the composition of the essential oil were identified, and the main components of the oil were camphene (19.45%) and α-pinene (13.85%). Cistus species, which are spread in many regions in Turkey, are produced, and if the amount and content of the chemical compositions of essential oils could be determined, they could be an alternative to food additives, synthetic antioxidants and active pharmaceutical ingredients.

Keywords

Cistus creticus L., pink rock rose, essential oil, altitude

Farklı Yüksekliklerden Toplanan Pembe Ladenin (Cistus creticus L.) Uçucu Yağ Oranı ve Kimyasal Bileşenlerinin Belirlenmesi

Abstract

Bu araştırma Tekirdağ ilinin Kumbağ, Yeniköy ve Uçmakdere mevkilerinde geniş bir alanda yayılış gösteren pembe laden bitkisinin (Cistus creticus L.) uçucu yağ oranı ve bu yağın kimyasal bileşimini belirlemek amacıyla 2019 yılında yürütülmüştür. Bu amaçla farklı yüksekliklerden toplanan (0, 50, 100 ve 150 m) pembe laden bitkisinin yaprakları oda sıcaklığında kurutulmuş ve numunelerden Clevenger cihazı yardımıyla uçucu yağ tayini yapılmıştır. Uçucu yağın kimyasal bileşimi GC/MS cihazı ile incelenmiştir. Araştırma sonucuna göre, uçucu yağ bileşiminin %73,03'ünü (≥ %1) temsil eden 13 bileşen tanımlanmış ve yağın ana bileşenlerini kamfen (%19.45) ve α-pinen (%13.85) oluşturmuştur. Ülkemizin birçok bölgesinde yayılış gösteren Cistus türlerinin üretimi yapılıp, uçucu yağlarının aktif bileşen miktarı ve içeriği belirlenebilirse gıda katkı maddelerine, sentetik antioksidanlara ve ilaç etken maddelerine doğal bir alternatif ürün olabilecekledir.

Keywords

Cistus creticus L., pembe laden, uçucu yağ, yükseklik

References

• Anonim, 2021. Tüylü laden. (Web sayfası: https://tr.wikipedia.org/wiki/Tüylü_laden) (Erişim tarihi: Mayıs 2021).
• Abu-Orabi, S.T., A. Mahmoud, R.S Noura, T.B. Tareq, M.O. Safwan, S. Mohammed, H.I. Al-Jaber, 2020. Antioxidant activity of crude extracts and essential oils from flower buds and leaves of Cistus creticus and Cistus salviifolius. Arabian Journal of Chemistry, 13: 6256-6266. https://doi.org/10.1016/j.arabjc.2020.05.043
• Agueda, B., J. Parladé, A.M. Miguel & F. Martínez-Peña, 2006. Characterization and identification of field ectomycorrhizae of Boletus edulis and Cistus ladanifer. Mycologia, 98 (1): 23-30. https://doi.org/10.1080/15572536.2006.11832709
• Arrington, J.M. & K. Kubitzki, 2003. “Cistaceae, 62-70”. In: The Families and Genera of Vascular Plants 5. (Eds: K. Kubitzki & C. Bayer), Springer, Berlin & Heidelberg, 418 pp.
• Bechlaghem, K., H. Allali, H. Benmehdi, N. Aissaoui & G. Flamini, 2019. Chemical analysis of the essential oils of three cistus species growing in North-West of Algeria. Agriculturae Conspectus Scientificusol, 84 (3): 283-293.
• Costa, R., M.R. De Fina, M.R. Valentino, M.L. Crupi & L. Mondello, 2009. Application of a new GC-MS library, designed with a retention İndex filter tool, to the analysis of the essential oil of Cistus ladanifer. Acta Horticulturae, 826: 271-276. https://doi.org/10.17660/ActaHortic.2009.826.37
• Demetzos, C., A. Loukis, V. Spiliotis, N. Zoakis, N. Stratigakis & H. Katerinopoulos, 1995. Composition and antimicrobial activity of the essential oil of Cistus creticus L. Journal of Essential Oil Research, 7 (4): 407-410. https://doi.org/10.1080/10412905.1995.9698549
• Demetzos, C., H. Katerinopoulos, A. Kouvarakis, N. Stratigakis, A. Loukis, C. Ekonomakis, V. Spiliotis & J. Tsaknis, 1997. Composition and antimicrobial activity of the essential oil of Cistus creticus subsp. eriocephalus. Planta Medica, 63 (5): 477-479. https://doi.org/10.1055/s-2006-957742
• Dirican, A. & İ. Telci, 2016. Tokat florasında doğal yayılış gösteren rezene popülasyonlarının morfolojik ve kalite özeliklerinin belirlenmesi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 53 (3): 293-299. https://doi.org/10.20289/zfdergi.389365
• Gomes, P.B., G. Vera, V.G. Mata & A.E. Rodrigues, 2005. Characterization of the Portuguese-grown Cistus ladanifer essential oil. Journal of Essential Oil Research, 17 (2): 160-165. https://doi.org/10.1080/10412905.2005.9698864
• Jerónimo, E., L. Cachucho, D. Soldado, O. Guerreiro, R.J.B. Bess & S.P. Alves, 2020. Fatty acid content and composition of the morphological fractions of Cistus Ladanifer L. and its seasonal variation. Molecules, 25 (7): 1550. https://doi.org/10.3390/molecules25071550
• Kılıç, D., B. Sırıken, Ö. Ertürk, G. Tanrıkulu, M. Gül & C. Başkan, 2019. Antibacterial, antioxidant and DNA interaction properties of Cistus creticus L. extracts. Journal of International Environmental Application and Science, 14 (3): 110-115.
• Kutlular, E., 2007. Bazı Adaçayı be Kekik Türlerinin Uçucu Yağlarının Süper Isıtılmış Su ile Ekstraksiyonları ve GC-MS ile Karakterizasyonları. Pamukkale Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 94 s, Denizli.
• Mohammed, B., C. Said, F.R. Fouzia, F.B. Kawtar, H. Zoubida, O. Abdelilah, E. Mohammed & E. Ghizlane, 2018. Chemical composition and antimicrobial activity of the essential oil of Cistus Ladanifer var. maculatus Dun. The Journal of Microbiology, Biotechnology and Food Sciences, 8 (3): 925-930. https://doi.org/10.15414/jmbfs.2018-19.8.3.925-930
• NIST, 2011. National Institute of Standards and Technology Standard Reference Data Program, For Use with Microsoft® Windows, Gaithersburg, ABD.
• Öğütveren, M. & Ş. Savaş Tetik, 2004a. Composition of the essential oil of Cistus laurifolius L. from Turkey. Journal of Essential Oil Research, 16 (1): 24-25. https://doi.org/10.1080/10412905.2004.9698641
• Öğütveren, M. & Ş. Savaş Tetik, 2004b. Composition of the essential oil of Cistus parviflorus L. from Turkey. Journal of Essential Oil Research, 16 (2): 115-116. https://doi.org/10.1080/10412905.2004.9698666
• Pattabanoğlu, E.S., 2018. Laurus nobilis ve Cistus laurifolius'dan Elde Edilen Uçucu Yağların GC-MS Analizi ve Antimikrobiyal Aktiviteleri. Kastamonu Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 50 s, Kastamonu.
• Polat, R. & F. Satil, 2012. An ethnobotanical survey of medicinal plants in Edremit Gulf (Balıkesir-Turkey). Journal of Ethnopharmacology, 139 (2): 626-641. https://doi.org/10.1016/j.jep.2011.12.004
• Politeoa, O., A. Maravić, F. Burčul, I. Careva & J. Kamenjarin, 2018. Phytochemical composition and antimicrobial activity of essential oils of wild growing Cistus species in Croatia. Natural Product Communications, 13 (6): 771-774. https://doi.org/10.1177/1934578X1801300631
• Sahraoui, R., S. Djellali & A.N. Chakera, 2013. Morphological, anatomical, secondary metabolites investigation and physicochemical analysis of Cistus creticus. Pharmacognosy Communications, 3 (4): 58-63. https://doi.org/10.5530/pc.2013.4.8
• Sargın, S.A. & S. Selvi, 2016. Türkiye’de yayılış gösteren Cistus L. (Cistaceae) cinsinin karşılaştırmalı yaprak anatomisi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 6 (2): 41-48.
• Seidakhmetova, R.B., A.A. Beisenbaeva, G.A. Atazhanova, E.M. Suleimenov, R.N. Pak, A.T. Kulyyasov & S.M. Adekenov, (2002). Chemical composition and biological activity of the essential oil from Artemisia glabella. Pharmaceutical Chemistry Journal, 36 (3): 135-138. https://doi.org/10.1023/A:1019630327576
• Şekeroğlu, N. & S. Gezici, 2020. Koronavirüs pandemisi ve Türkiye’nin bazı şifalı bitkileri. Anadolu Kliniği Tıp Bilimleri Dergisi, COVID 19 Özel Sayısı: 163-182. https://doi.org/10.21673/anadoluklin.724210
• Şekeroğlu, N. & S. Gezici, 2021. Türkiye’nin virüs savar bitkisi laden (Cistus spp.) türleri: Geleneksel kullanımları, biyoaktif kimyasal bileşenleri ve farmakolojik aktiviteleri. Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi, 11 (2): 258-268. https://doi.org/10.31020/mutftd.895397
• TÜBİVES, 2021. Türkiye bitkileri veri servisi, (Web sayfası: http://www.tubives.com/) (Erişim tarihi: Mayıs 2021).
• Verdeguer, M., M. Amparo Blázquez & H. Boira, 2012. Chemical composition and herbicidal activity of the essential oil from a Cistus ladanifer L. population from Spain. Natural Product Research, 26 (17): 1602-1609. https://doi.org/10.1080/14786419.2011.592835
• Viuda-Martos, M., E. Sendra, J.A. Pérez-Alvarez, J. Fernández-López, M. Amensour & J. Abrini, 2011. Identification of flavonoid content and chemical composition of the essential oils of Moroccan herbs: Myrtle (Myrtus communis L.), Rockrose (Cistus ladanifer L.) and Montpellier cistus (Cistus monspeliensis L.). Journal of Essential Oil Research, 23 (2): 1-9. https://doi.org/10.1080/10412905.2011.9700439
• Yeşilada, E., İ. Gürbüz & E. Ergün, 1997. Effects of Cistus laurifolius L. flowers on gastric and duodenal lesions. Journal of Ethnopharmacology, 55 (3): 201-211. https://doi.org/10.1016/s0378-8741(96)01502-4
• Zidane, H., M. Elmiz, F. Aouinti, A. Tahani, J. Wathelet, M. Sindic & A. Elbachiri, 2013. Chemical composition and antioxidant activity of essential oil, various organic extracts of Cistus ladanifer and Cistus libanotis growing in Eastern Morocco. African Journal of Biotechnology, 12 (34): 5314-5320. https://doi.org/10.5897/AJB2013.12868
• Zidane, H., M.L. Fauconnier, M. Sindic & A. El Bachiri, 2014. Comparative study of volatile secondary metabolite of Cistus libanotis during different process. Journal of Chemical and Pharmaceutical Research, 6 (8): 281-287.