Potential Role of Laurus nobilis Essential Oil in Reducing Indomethacin-Induced Gastric Ulcer in Rats

Abstract

Objective: This study aimed to investigate the gastroprotective effects of Laurus nobilis leaves essential oil (LANO) against indomethacin (INDO)-induced gastric ulcers in rats. Materials and Methods: In this study, an indomethacin-induced gastric ulcer model was employed. 30 Sprague-Dawley rats were divided into five groups (n=6): Control, LANO, INDO, INDO with famotidine (FAM), and INDO with LANO. Indomethacin (25 mg/kg) induced ulcers, while LANO and FAM were administered by oral gavage at 200 mg/kg and 40 mg/kg, respectively. Gastric tissues underwent histopathological examination for ulceration, and biochemical assays measured total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and nitric oxide (NO) levels. Results: Compared to the INDO group, treatment with LANO significantly decreased the number of gastric ulcer foci. Biochemically, LANO moderated TOS and OSI levels and preserved TAS, indicating reduced oxidative stress. Additionally, LANO appeared to stabilize NO levels. These biochemical findings were corroborated by histopathological examination. Conclusions: The study's results indicate that LANO may be beneficial in protecting against NSAID-induced gastric damage. LANO's observed modulation of oxidative stress markers and NO levels suggests its potential role in managing gastric ulcers.

Keywords

• Gastric ulcer
• indomethacin
• Laurus nobilis
• nitric oxide
• oxidative stress

Laurus nobilis Esansiyel Yağının Sıçanlarda İndometasin Kaynaklı Mide Ülserinin Azaltılmasında Potansiyel Rolü

Öz

Amaç: Bu çalışmanın amacı, sıçanlarda indometasin (INDO) ile indüklenen gastrik ülserlere karşı Laurus nobilis yaprakları uçucu yağının (LANO) gastroprotektif etkilerini araştırmaktır. Materyal ve Metot: Bu çalışmada, INDO ile indüklenen gastrik ülser modeli kullanıldı. 30 adet Sprague-Dawley sıçan beş gruba ayrıldı (n=6): Kontrol, LANO, INDO, famotidin (FAM) ile INDO, LANO ile INDO. INDO (25 mg/kg) ülserleri indüklerken, LANO ve FAM oral gavaj yoluyla sırasıyla 200 mg/kg ve 40 mg/kg olarak uygulandı. Gastrik dokular ülserasyon açısından histopatolojik incelemeye tabi tutuldu ve biyokimyasal analizlerle toplam oksidan durum (TOS), toplam antioksidan durum (TAS), oksidatif stres indeksi (OSI) ve nitrik oksit (NO) seviyeleri ölçüldü. Bulgular: İndometasin grubuyla karşılaştırıldığında, LANO ile tedavi gastrik ülser odaklarının sayısını önemli ölçüde azalttı. Biyokimyasal olarak, LANO uygulaması TOS ve OSI seviyelerini düşürdü ve TAS seviyesini korudu, bu da oksidatif stresin azaldığını göstermektedir. Ayrıca, LANO'nun NO seviyelerini stabilize ettiği görüldü. Bu biyokimyasal bulgular histopatolojik inceleme ile desteklendi. Sonuç: Çalışmanın sonuçları, LANO'nun INDO kaynaklı mide hasarına karşı korunmada faydalı olabileceğini göstermektedir. LANO'nun oksidatif stres belirteçleri ve NO seviyelerinde gözlenen modülasyonu, mide ülserlerinin yönetiminde potansiyel rolünü ortaya koymaktadır.

Anahtar Kelimeler

• İndometasin
• Laurus nobilis
• mide ülseri
• nitrik oksit
• oksidatif stres

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