Beta Vulgaris L. Extract: pH Effect on Total Phenolic Content and Antioxidant Properties

Year 2024, Volume: 28 Issue: 3, 589 - 593, 30.06.2024

Rama Alkayarı Zuhal Şahin Fatih Sonmez Mustafa Küçükislamoğlu

https://doi.org/10.16984/saufenbilder.1381328

Abstract

Red beet (Beta vulgaris L.) is a rich source of phenolic content including colour pigments and have also high antioxidant capacities. The amounts of their phenolic substances and antioxidant activities change depending on the extraction conditions (pH, time, solvent, etc.). In this work, the coloured water extracts were obtained from red beet at different pH values (pH 4-10) for both an hour and 24 hours. The extracts were evaluated regarding antioxidant activities and total phenolic contents (TPC). The results showed that the total phenolic content of red beet extract in all extraction conditions ranged from 0.55±0.02 to 2.30±0.19 mg GAE/g FW. The highest total phenolic contents (2.30±0.19 mg GAE/g FW) were obtained from red beet at 24 h and pH=4. On the other hand, while the IC50 values for DPPH activity of red beet extracts at all pH values and times are between 0.84±0.04 and 5.44±0.75 mg/mL, the IC50 values for ABTS activity are between 1.46±0.42 and 3.65± 0.28 mg/mL. The extract obtained from red beet at 24 h and pH=4 exhibited the strongest DPPH activity with the IC50 values of 0.84±0.04 mg/mL, it showed the best ABTS activity with the IC50 values of 1.46±0.42 mg/mL at 24 h and pH=4.

Keywords

Beta vulgaris L, pH effect, Antioxidant activity, TPC

Supporting Institution

This work was supported by Research Fund of the Sakarya University of Applied Sciences (Project Number: 107-2022)

References

• [1] F.C. Stintzing, R. Carle, “Functional properties of anthocyanins and betalains in plants, food, and in human nutrition,” Trends in food science & technology, vol. 15, no. 1, pp. 19–38, 2004.
• [2] I. Amao, "Health benefits of fruits and vegetables: Review from Sub-Saharan Africa" Vegetables: importance of quality vegetables to human health, vol. 22, pp. 33-53, 2018.
• [3] T.G. Ulger, A.N. Songur, O. Cırak, “Role of Vegetables in Human Nutrition and Disease Prevention,” Intechopen, pp. 7-32, 2018.
• [4] R. Karl, M.A. Koch, “A world-wide perspective on crucifer speciation and evolution: phylogeny, biogeography and trait evolution in tribe Arabideae,” Annals of Botany, vol. 112, no. 6, pp. 983–1001, 2013.
• [5] I. Sadowska-Bartosz, G. Bartosz, “Biological properties and applications of betalains,” Molecules, vol. 26, no. 9, pp. 2520, 2021.
• [6] N. Chhikara, K. Kushwaha, P. Sharma, Y. Gat, A. Panghal, “Bioactive compounds of beetroot and utilization in food processing industry: A critical review,” Food chemistry, vol. 272, pp. 192−200, 2019.
• [7] L. Sharma, M. Sardana, “Organoleptic properties of a standardised food product (cookies) developed from beet root extract and bengal gram flour,” International Journal of Food, Nutrition and Dietetics, vol. 5, no. 2, pp. 51-57, 2017.
• [8] T. Clifford, G. Howatson, D.J. West, E.J. Stevenson, “The potential benefits of red beetroot supplementation in health and disease,” Nutrients, vol. 7, no. 4, pp. 2801-2822, 2015.
• [9] D. Strack, T. Vogt, W. Schliemann, “Recent advances in betalain research” Phytochem, vol. 62, no. 3, pp. 247-269, 2003.
• [10] K. Ozcan, S.E. Bilek, “Production and stability of food colorant from red beetroot,” Akademik Gıda, vol. 16, no. 4, pp. 439-449, 2018.
• [11] V.H. Ozyurt, H. Saralı, S. Otles, “Usage of betalain extracts in food,” Pamukkale University Journal of Engineering Sciences, vol. 25, no. 7, pp. 864-870, 2019.
• [12] F. Sonmez, Z. Sahin, “Comparative study of total phenolic content, antioxidant activities, and polyphenol oxidase enzyme inhibition of quince leaf, peel, and seed extracts” Erwerbs-Obstbau, vol. 65, no. 4, pp. 745-750, 2023.
• [13] F. Sonmez, Z. Gunesli, B.Z. Kurt, I. Gazioglu, D. Avci & M. Kucukislamoglu “Synthesis, antioxidant activity and SAR study of novel spiro-isatin-based Schiff bases” Molecular Diversity, vol. 23, pp. 829–844, 2019.
• [14] F. Sonmez, Z. Gunesli, T. Demir, K. Cıkrıkcı, A. Ergun, N. Gencer, O. Arslan, “The effect of total anthocyanins extracted from sweet cherry cultivars on carbonic anhydrases and antioxidant activity,” Erwerbs-Obstbau, vol. 64, pp. 145-153, 2022.