Platismatia glauca (L.) W.L.Culb. & C.F.Culb.'nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri

Buğrahan Emsen; Ali Aslan; Abdullah Kaya

doi:10.19113/sdufbed.00743

Platismatia glauca (L.) W.L.Culb. & C.F.Culb.'nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri

Abstract

Likenler yapısında farklı metabolitleri bulunduran ve bu bileşenleri sayesinde antik çağlardan beri birçok hastalığın tedavisinde yararlanılan organizma olarak dikkat çekmektedir. Bu yüzden, mevcut çalışma önemli liken türlerinden biri olan Platismatia glauca (L.) W.L.Culb. & C.F.Culb.'nın insan lenfositleri üzerindeki etkilerini incelemeyi amaçlamıştır. P. glauca'dan elde edilen metanol (PME) ve su (PSE) ekstraktlarının hücreler üzerindeki sitotoksik aktiviteleri 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür ve laktat dehidrogenaz testleri ile analiz edilmiştir. PME ve PSE ile muamele edilen lenfositlerdeki oksidatif değişimin belirlenmesi amacıyla toplam antioksidan kapasite (TAK) ve toplam oksidan durum (TOD) analizleri gerçekleştirilmiştir. Bu uygulamalara ek olarak, ekstraktların hücreler üzerinde sebep olduğu genetik hasar hücrelerdeki 8-hidroksi-2′-deoksiguanozin seviyeleri ölçülerek tespit edilmiştir. Hesaplanan IC₅₀değerleri,PSE'nin (162,26 mg/L) PME'ye (84,02 mg/L) kıyasla çok daha düşük seviyede sitotoksik aktivite gösterdiğini ortaya çıkarmıştır. Her iki ekstraktın tüm konsantrasyonlarının (6,25-200 mg/L) negatif kontrole kıyasla istatistiksel olarak (p>0,05) hücrelerdeki TAK'yi yükselttiği ve aynı zamanda düşük konsantrasyonlarının genetik hasar meydana getirmediği belirlenmiştir. Tüm uygulamalar göz önüne alındığında, PME ve özellikle PSE'nin belli konsantrasyonlarının (<25 mg/L) lenfositler üzerinde anlamlı derecede oksidatif ve genetik hasara neden olmadan TAK'yi yükselttiği ve bu nedenle P. glauca likeninin doğal bir antioksidan olarak kullanılabileceği gözlenmiştir.

Keywords

Antioksidan,Genotoksisite; Oksidatif stres; Sitotoksisite

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Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Buğrahan Emsen

Ali Aslan

Abdullah Kaya

Publication Date

August 15, 2018

Submission Date

July 15, 2017

Acceptance Date

-

Published in Issue

Year 2018 Volume: 22 Number: 2

DOI

https://doi.org/10.19113/sdufbed.00743

IZ

https://izlik.org/JA24CJ92UF

Cite

RIS / Bibtex

APA

Emsen, B., Aslan, A., & Kaya, A. (2018). Platismatia glauca (L.) W.L.Culb. & C.F.Culb.’nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22(2), 840-848. https://doi.org/10.19113/sdufbed.00743

AMA

1.Emsen B, Aslan A, Kaya A. Platismatia glauca (L.) W.L.Culb. & C.F.Culb.’nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri. J. Nat. Appl. Sci. 2018;22(2):840-848. doi:10.19113/sdufbed.00743

Chicago

Emsen, Buğrahan, Ali Aslan, and Abdullah Kaya. 2018. “Platismatia Glauca (L.) W.L.Culb. & C.F.Culb.’nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 (2): 840-48. https://doi.org/10.19113/sdufbed.00743.

EndNote

Emsen B, Aslan A, Kaya A (August 1, 2018) Platismatia glauca (L.) W.L.Culb. & C.F.Culb.’nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 2 840–848.

IEEE

[1]B. Emsen, A. Aslan, and A. Kaya, “Platismatia glauca (L.) W.L.Culb. & C.F.Culb.’nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri”, J. Nat. Appl. Sci., vol. 22, no. 2, pp. 840–848, Aug. 2018, doi: 10.19113/sdufbed.00743.

ISNAD

Emsen, Buğrahan - Aslan, Ali - Kaya, Abdullah. “Platismatia Glauca (L.) W.L.Culb. & C.F.Culb.’nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22/2 (August 1, 2018): 840-848. https://doi.org/10.19113/sdufbed.00743.

JAMA

1.Emsen B, Aslan A, Kaya A. Platismatia glauca (L.) W.L.Culb. & C.F.Culb.’nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri. J. Nat. Appl. Sci. 2018;22:840–848.

MLA

Emsen, Buğrahan, et al. “Platismatia Glauca (L.) W.L.Culb. & C.F.Culb.’nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 22, no. 2, Aug. 2018, pp. 840-8, doi:10.19113/sdufbed.00743.

Vancouver

1.Buğrahan Emsen, Ali Aslan, Abdullah Kaya. Platismatia glauca (L.) W.L.Culb. & C.F.Culb.’nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri. J. Nat. Appl. Sci. 2018 Aug. 1;22(2):840-8. doi:10.19113/sdufbed.00743

Cited By

Yabani Yenilebilir Bir Mantar Olan Lycoperdon molle Pers.'nin Antioksidan ve Antigenotoksik Potansiyeli

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.v22i45606.523756

<i>Platismatia glauca</i> (L.) W.L.Culb. & C.F.Culb.'nın İnsan Lenfositleri Üzerindeki Biyolojik Aktiviteleri