Histological and Histochemical Investigation of the Stomach Mucosa of Muraena helena Linnaeus, 1758 (Mediterranean Moray Fish)

Abstract

In this study, it was aimed to determine the histological structure and histochemical properties of gastric mucosa of Muraena helena. For this purpose, samples taken from cardia, fundus and pylorus which from the stomach regions of Muraena helena (N=10) were examined. Tissue samples taken at autopsy were fixed in 10 % neutral formaldehyde solution. After the routine histological tissue following procedure stages, tissues were taken into paraffin blocks and 5 µthick sections were prepared from the blocks. The preparations were stained with Hematoxylin-Eosin (H-E), Aldehyde Fuchsin (AF), Periodic Acid Schiff (PAS), KOH/PAS, Alcian Blue (AB) pH 0.5, AB 0.3 M, AB 0.06 M, AB pH 2.5, AF/AB pH 2.5, AB pH 1.0, PAS/AB pH 2.5 staining methods and photographed. In the tissue sections examined, it was observed that the stomach mucosa consists of four layers as tunica mucosa, submucosa, tunica muscularis and tunica serosa. With the histochemical staining methods applied, it was determined that the cardia, fundus and pylorus regions showed sulphate acidic, acidic sulphate and carboxylated acidic mucosubstances.

Keywords

• Mediterranean morayeel
• Muraena helena
• Histochemistry
• Mucosa
• Mukosubstance

Muraena helena Linnaeus, 1758 (Akdeniz Müren Balığı)’nın Mide Mukozasının Histolojik ve Histokimyasal Olarak İncelenmesi

Abstract

Çalışmada Muraena helena’nın mide mukozasının, histolojik yapısı ve histokimyasal özelliklerinin belirlenmesi amaçlanmıştır. Bu amaçla Muraena helena (N= 10)’ nın mide bölgelerini oluşturan kardiya, fundus ve pilorustan alınan örnekler incelenmiştir. Otopsi ile alınan doku örnekleri %10 nötral formaldehit solüsyonunda tespit edilmiştir. Rutin histolojik doku takip aşamalarından sonra dokular parafin bloklara alınarak bloklardan 5 µm kalınlığında kesitler hazırlanmıştır. Preparatlar Hematoksilen-Eosin (H-E), Aldehyde Fuchsin (AF), Periodic Acid Schiff (PAS), KOH, Alcian Blue (AB) pH 0.5, AB 0.3 M, AB 0.06 M, AB pH 2.5, AF/AB pH 2.5, AB pH 1.0, PAS/ AB pH 2.5 boyama yöntemleri ile boyanmış ve fotoğraflanmıştır. İncelenen doku kesitlerinde, mide mukozasının tunika mukoza, submukoza, tunika muskularis ve tunika seroza olarak dört katmandan oluştuğu gözlenmiştir. Uygulanan histokimyasal boyama yöntemleri ile kardiya, fundus ve pilorus bölgelerinin güçlü düzeyde sülfatlı asidik, asidik, sülfatlı ve karboksilli asidik mukosubstans özelliği gösterdiği tespit edilmiştir.

Keywords

• Muraena helena
• Akdeniz Müreni
• Mukosubstans
• Mukoza
• Histokimya

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