SHİLAJİT’İN HIZLI MAKSİLLER GENİŞLETME TEDAVİSİNDE YENİ KEMİK ŞEKİLLENMESİ ÜZERİNE ETKİSİ VAR MI? BİYOKİMYASAL, HİSTOPATOLOJİK VE İMMÜNOHİSTOKİMYASAL BİR ÇALIŞMA

Year 2019, , 96 - 103, 04.03.2019

Mine Geçgelen Cesur , Gozde Ogrenim Kanat Gulle , Fevziye Burcu Sirin Meryem Akpolat Gokhan Cesur

https://doi.org/10.17343/sdutfd.511364

Cited By: 5

Abstract

Amaç: Bu
çalışmanın amacı, rat çalışma modelinde shilajit'in hızlı maksiller genişletme
(RME) sonrası yeni kemik oluşumuna etkilerini biyokimyasal, histolojik ve
immünohistokimyasal teknikler kullanarak araştırmaktır.

Gereç ve Yöntem: Ratlar
(12 haftalık, 24 erkek Wistar albino) rastgele olarak 3 gruba ayrılmıştır (her
bir grupta n = 8): genişletme yapılmamış (NE), sadece genişletme yapılmış (OE),
genişletmeye ilave olarak shilajit uygulanmış (Shilajit). Ratlara 5 günlük
genişletme ve 12 günlük retansiyon periyodu süresince Shilajit verildi.
Hayvanlar sakrifiye edildikten sonra biyokimyasal, histolojik ve
immünohistokimyasal incelemeler yapıldı.

Bulgular: Shilajit
grubundaki süperoksit dismutaz, katalaz ve glutatyon peroksidaz düzeyleri OE
grubundan istatistiksel olarak daha yüksekti (p <0.05). Kemik alkalen
fosfataz ve C-terminal telopeptid tip I kollajen seviyelerinde, gruplar
arasında istatistiksel olarak anlamlı farklılıklar bulundu (p <0.001).
İmmünohistokimyasal bulgular, OE grubunun shilajit grubundan anlamlı olarak
daha fazla IL-1 ve TNF-α H skorlarına sahip olduğunu gösterdi (p <0.05).
Gruplar inflamatuar hücre infiltrasyonu, yeni kemik oluşumu ve kılcal damar
yoğunluğu açısından karşılaştırıldığında gruplar arasında önemli farklılıklar
bulundu (p <0.05).

Sonuç: Shilajit'in
sistemik kullanımı, midpalatal suturada yeni kemik oluşumunu hızlandırarak, RME
tedavisinden sonra nüksün önlenmesinde ve retansiyon süresini kısaltmada faydalı
olabilir.

Keywords

Hızlı maksiller genişletme, Shilajit, Relaps

DOES SHILAJIT HAVE AN EFFECT ON NEW BONE REMODELLING IN THE RAPID MAXILLARY EXPANSION TREATMENT? A BIOCHEMICAL, HISTOPATHOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY

Abstract

Aim: The
aim of this study was to investigate the effects of shilajit on new bone
formation following rapid maxillary expansion (RME) in a rat study model using biochemical, histological, and immunohistochemical techniques.

Material and Method: The
rats (12-week-old, 24
male Wistar albino) were randomly divided into the following 3 groups
(n=8 each): no expansion (NE), only expansion (OE), expansion plus shilajit (Shilajit). Shilajit was
given to the rats during the 5 day expansion and 12 day
retention period. After sacrificing the animals, biochemical, histological,
and immunohistochemical examinations were
performed.

Results: Superoxide
dismutase, catalase,
and glutathione peroxidase levels
in the shilajit group were statistically higher than the OE group (p<0.05). Bone
alkaline phosphatase and C-telopeptide of type I collagen levels demonstrated statistically significant differences
between the groups (p<0.001).  The
immunohistochemical findings revealed that OE group had significantly more IL-1 and
TNF-α H scores than the shilajit group (p<0.05). When
the groups were compared for inflammatory
cell infiltration, new bone formation, and capillary
intensity, considerable differences were found between the groups (p<0.05).

Conclusion: Systemic
use of shilajit may hasten new bone
formation in the midpalatal suture, which may be useful to prevent of relapse and shorten the
retention period after the RME treatment.

Keywords

Rapid maxillary expansion, Shilajit, Relapse

References

• 1-Aras MH, Erkilic S, Demir T, Demirkol M, Kaplan DS, Yolcu U. Effects of low-level laser therapy on osteoblastic bone formation and relapse in an experimental rapid maxillary expansion model. Niger J Clin Pract 2015;18:607-11.
• 2-Kara MI, Erciyas K, Altan AB, Ozkut M, Ay S, Inan S. Thymoquinone accelerates new bone formation in the rapid maxillary expansion procedure. Arch Oral Biol 2012;57:357-63.
• 3-Buyuk SK, Ramoglu SI, Sonmez MF. The effect of different concentrations of topical ozone administration on bone formation in orthopedically expanded suture in rats. Eur J Orthod 2016;38:281-5.
• 4-Özan F, Çörekçi B, Toptaş O, Halicioğlu K, Irgin C, Yilmaz F, et al. Effect of Royal Jelly on new bone formation in rapid maxillary expansion in rats. Med Oral Patol Oral Cir Bucal 2015;20:651-6.
• 5-Zhao S, Wang X, Li N, Chen Y, Su Y, Zhang J. Effects of strontium ranelate on bone formation in the mid-palatal suture after rapid maxillary expansion. Drug Des Devel Ther 2015;9:2725-34.
• 6-Oztürk F, Babacan H, Inan S, Gümüş C. Effects of bisphosphonates on sutural bone formation and relapse: A histologic and immunohistochemical study. Am J Orthod Dentofacial Orthop 2011;140:31-41.
• 7-Uysal T, Amasyali M, Enhos S, Sonmez MF, Sagdic M. Effect of ED-71, a new active vitamin D analog, on bone formation in an orthopedically expanded suture in rats. A Histomorphometric Study. Eur J Dent 2009;3:165-72.
• 8-Farhadian N, Miresmaeili AR, Zargaran M, Moghimbeigi A, Soheilifar S. Effect of dietary ascorbic acid on osteogenesis of expanding midpalatal suture in rats. J Dent (Tehran) 2015;12:39-48.
• 9-Uysal T, Amasyali M, Enhos S, Karslioglu Y, Yilmaz F, Gunhan O. Effect of periosteal stimulation therapy on bone formation in orthopedically expanded suture in rats. Orthod Craniofac Res 2010;13:89-95.
• 10-Da Silva AP, Petri AD, Crippa GE, Stuani AS, Stuani AS, Rosa AL, et al. Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells. Lasers Med Sci 2012;27:777-83.
• 11-Aras MH, Bozdag Z, Demir T, Oksayan R, Yanık S, Sökücü O. Effects of low-level laser therapy on changes in inflammation and in the activity of osteoblasts in the expanded premaxillary suture in an ovariectomized rat model. Photomed Laser Surg 2015;33,136-44.
• 12-Amini F, Najaf Abadi MP, Mollaei M. Evaluating the effect of laser irradiation on bone regeneration in midpalatal suture concurrent to rapid palatal expansion in rats. J Orthod Sci 2015;4:65-71.
• 13-Rosa CB, Habib FA, de Araújo TM, Dos Santos JN, Cangussu MC, Barbosa AF, et al. Laser and LED phototherapy on midpalatal suture after rapid maxilla expansion: Raman and histological analysis. Lasers Med Sci 2017;32:263-74.
• 14-Sawada M, Shimizu N. Stimulation of bone formation in the expanding mid-palatal suture by transforming growth factor-beta 1 in the rat. Eur J Orthod 1996;18:169-79.
• 15-Ekizer A, Yalvac ME, Uysal T, Sonmez MF, Sahin F. Bone marrow mesenchymal stem cells enhance bone formation in orthodontically expanded maxillae in rats. Angle Orthod 2015;85:394-9.
• 16-Uysal T, Amasyali M, Olmez H, Enhos S, Karslioglu Y, Gunhan O. Effect of vitamin C on bone formation in the expanded inter-premaxillary suture. Early bone changes. J Orofac Orthop 2011;72:290-300.
• 17-Tang GH, Xu J, Chen RJ, Qian YF, Shen G. Lithium delivery enhances bone growth during midpalatal expansion. J Dent Res 2011;90:336-40.
• 18-Toy E, Oztürk F, Altindiş S, Kozacioğlu S, Toy H. Effects of low-intensity pulsed ultrasound on bone formation after the expansion of the inter-premaxillary suture in rats: a histologic and immunohistochemical study. Aust Orthod J 2014;30:176-83.
• 19-Birlik M, Babacan H, Cevit R, Gürler B. Effect of sex steroids on bone formation in an orthopedically expanded suture in rats: An immunohistochemical and computed tomography study. J Orofac Orthop 2016;77:94-103.
• 20-Sadikoglu TB, Nalbantgil D, Ulkur F, Ulas N. Effect of hyaluronic acid on bone formation in the expanded interpremaxillary suture in rats. Orthod Craniofac Res 2016;1:154-61.
• 21-Uysal T, Gorgulu S, Yagci A, Karslioglu Y, Gunhan O, Sagdic D. Effect of resveratrol on bone formation in the expanded inter-premaxillary suture: early bone changes. Orthod Craniofac Res 2011;14:80-7.
• 22-Altan BA, Kara IM, Nalcaci R, Ozan F, Erdogan SM, Ozkut MM, et al. Systemic propolis stimulates new bone formation at the expanded suture: a histomorphometric study. Angle Orthod 2013;83:286-91.
• 23-Kara MI, Altan AB, Sezer U, Erdoğan MŞ, Inan S, Ozkut M, et al. Effects of Ginkgo biloba on experimental rapid maxillary expansion model: a histomorphometric study. Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114:712-8.
• 24-Kececi M, Akpolat M, Gulle K, Gencer E, Sahbaz A. Evaluation of preventive effect of shilajit on radiation-induced apoptosis on ovaries. Arch Gynecol Obstet 2016;293:1255-62.
• 25-Agarwal SP, Khanna R, Karmarkar R, Anwer MK, Khar RK. Shilajit: a review. Phytother Res 2007;21:401-5. 26-Wilson E, Rajamanickam GV, Dubey GP, Klose P, Musial F, Saha FJ, et al. Review on shilajit used in traditional Indian medicine. J Ethnopharmacol 2011;136:1-9. 27-Velmurugan C, Vivek B, Wilson E, Bharathi T, Sundaram T. Evaluation of safety profile of black shilajit after 91 days repeated administration in rats. Asian Pac J Trop Biomed 2012;2:210-4.
• 28-Stohs SJ. Safety and efficacy of shilajit (mumie, moomiyo). Phytother Res 2014;28:475-9.
• 29-Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1996;186:421-31.
• 30-Woolliams JA, Wiener G, Anderson PH, McMurray CH. Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of copper in the blood various breed crosses of sheep. Res Vet Sci 1983;34:69-77.
• 31-Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70:158-69. 32-Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
• 33-Irgin C, Çörekçi B, Ozan F, Halicioğlu K, Toptaş O, Birinci Yildirim A, et al. Does stinging nettle (Urtica dioica) have an effect on bone formation in the expanded inter-premaxillary suture? Arch Oral Biol 2016;69:13-8.
• 34-Jung CR, Schepetkin IA, Woo SB, Khlebnikov AI, Kwon BS. Osteoblastic differentiation of mesenchymal stem cells by mumie extract. Drug Dev Res 2002;57:122-33.
• 35-Lee CY, Suzuki JB. CTX biochemical marker of bone metabolism. Is it a reliable predictor of bisphosphonate-associated osteonecrosis of the jaws after surgery? Part II: a prospective clinical study. Implant Dent 2010;19:29-38.
• 36-Garrett IR, Boyce BF, Oreffo RO, Bonewald L, Poser J, Mundy GR. Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro and in vivo. J Clin Invest 1990;85:632-9.
• 37-Houghton PJ, Zarka R, de las Heras B, Hoult JR. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 1995;61:33-6.
• 38-Halicioglu K, Corekci B, Akkas I, Irgin C, Ozan F, Yilmaz F, et al. Effect of St John’s wort on bone formation in the orthopaedically expanded premaxillary suture in rats: a histological study. Eur J Orthod 2015;37:164-9.