SHİLAJİT’İN HIZLI MAKSİLLER GENİŞLETME TEDAVİSİNDE YENİ KEMİK ŞEKİLLENMESİ ÜZERİNE ETKİSİ VAR MI? BİYOKİMYASAL, HİSTOPATOLOJİK VE İMMÜNOHİSTOKİMYASAL BİR ÇALIŞMA

Öz

Amaç: Bu çalışmanın amacı, rat çalışma modelinde shilajit'in hızlı maksiller genişletme (RME) sonrası yeni kemik oluşumuna etkilerini biyokimyasal, histolojik ve immünohistokimyasal teknikler kullanarak araştırmaktır.

Gereç ve Yöntem: Ratlar (12 haftalık, 24 erkek Wistar albino) rastgele olarak 3 gruba ayrılmıştır (her bir grupta n = 8): genişletme yapılmamış (NE), sadece genişletme yapılmış (OE), genişletmeye ilave olarak shilajit uygulanmış (Shilajit). Ratlara 5 günlük genişletme ve 12 günlük retansiyon periyodu süresince Shilajit verildi. Hayvanlar sakrifiye edildikten sonra biyokimyasal, histolojik ve immünohistokimyasal incelemeler yapıldı.

Bulgular: Shilajit grubundaki süperoksit dismutaz, katalaz ve glutatyon peroksidaz düzeyleri OE grubundan istatistiksel olarak daha yüksekti (p <0.05). Kemik alkalen fosfataz ve C-terminal telopeptid tip I kollajen seviyelerinde, gruplar arasında istatistiksel olarak anlamlı farklılıklar bulundu (p <0.001). İmmünohistokimyasal bulgular, OE grubunun shilajit grubundan anlamlı olarak daha fazla IL-1 ve TNF-α H skorlarına sahip olduğunu gösterdi (p <0.05). Gruplar inflamatuar hücre infiltrasyonu, yeni kemik oluşumu ve kılcal damar yoğunluğu açısından karşılaştırıldığında gruplar arasında önemli farklılıklar bulundu (p <0.05).

Sonuç: Shilajit'in sistemik kullanımı, midpalatal suturada yeni kemik oluşumunu hızlandırarak, RME tedavisinden sonra nüksün önlenmesinde ve retansiyon süresini kısaltmada faydalı olabilir.

Anahtar Kelimeler

• Hızlı maksiller genişletme
• Shilajit
• Relaps

DOES SHILAJIT HAVE AN EFFECT ON NEW BONE REMODELLING IN THE RAPID MAXILLARY EXPANSION TREATMENT? A BIOCHEMICAL, HISTOPATHOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY

Öz

Aim: The aim of this study was to investigate the effects of shilajit on new bone formation following rapid maxillary expansion (RME) in a rat study model using biochemical, histological, and immunohistochemical techniques.

Material and Method: The rats (12-week-old, 24 male Wistar albino) were randomly divided into the following 3 groups (n=8 each): no expansion (NE), only expansion (OE), expansion plus shilajit (Shilajit). Shilajit was given to the rats during the 5 day expansion and 12 day retention period. After sacrificing the animals, biochemical, histological, and immunohistochemical examinations were performed.

Results: Superoxide dismutase, catalase, and glutathione peroxidase levels in the shilajit group were statistically higher than the OE group (p<0.05). Bone alkaline phosphatase and C-telopeptide of type I collagen levels demonstrated statistically significant differences between the groups (p<0.001).  The immunohistochemical findings revealed that OE group had significantly more IL-1 and TNF-α H scores than the shilajit group (p<0.05). When the groups were compared for inflammatory cell infiltration, new bone formation, and capillary intensity, considerable differences were found between the groups (p<0.05).

Conclusion: Systemic use of shilajit may hasten new bone formation in the midpalatal suture, which may be useful to prevent of relapse and shorten the retention period after the RME treatment.

Anahtar Kelimeler

• Rapid maxillary expansion
• Shilajit
• Relapse

Kaynakça

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