Bazı Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae: Aphidiinae) popülasyonlarında genetik çeşitlilik

Year 2018, Volume: 9 Issue: 2, 124 - 136, 03.12.2018

Gül Satar Petek Toklu

https://doi.org/10.31019/tbmd.478059

Cited By: 1

Abstract

Lysiphlebus
confusus Tremblay & Eady
(Hymenoptera: Braconidae: Aphidiinae), geniş bir konukçu dizisine sahip önemli
bir yaprak biti parazitoididir. L.
confusus'taki genetik çeşitlilik, Adana ve Hatay illerinde konukçu yaprak
biti, konukçu bitki ve yükseklik açısından PCR-RFLP yöntemi ile incelenmiştir. PCR-RFLP
yöntemi ile SacI, AccI, HpyCH4III ve EcoP151 enzimlerinin 709 bp mitokondriyal
COI gen bölgesinde enzim kesim noktaları saptanmıştır. Gen dizileri diğer
ülkelerden (Sırbistan, Çin, Fransa ve İran) olan örnekler karşılaştırıldı ve
net sapmaları belirlendi. Balcalı (11) ve Ceyhan (4) örnekleri İran örneğine
yakınken, Erzin (5) Sırp ve diğer İran örneğine yakın olarak bulundu.
Seyhan'dan Cucumis melo L, Gülek
(Pozantı) den Solanum nigrum L.,
Saimbeyli'den Citrullus lanatus
(Tunb.), Ceyhan'dan S. nigrum ve
Balcalı'dan Vicia sativa L. örnekleri,
PCR-RFLP ile sırasıyla SacI, HpyCH4III,
EcoP151 ve EcoP151 ile AccI enzimleri tarafından sırayla
kesildiği belirlendi yükseklik, konukçu afit ve bitki türlerine bağlı spesifik
genetik çeşitlilik saptanmadı.

Keywords

Genetik çeşitlilik, Braconidae, PCR-RFLP, biyolojik kontrol

Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations

Abstract

Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae: Aphidiinae) is an
important aphid parasitoid that has a wide host range. Genetic diversity in L. confusus was studied by the PCR-RFLP
method in terms of host aphid, host plant, and altitude in Adana and Hatay
Provinces, Turkey. Restriction sites of SacI, AccI, HpyCH4III, and EcoP151
enzymes on the 709 bp mitochondrial COI gene region was detected by PCR-RFLP
method. The sequences were compared with specimens from other countries
(Serbia, China, France and Iran) and their net divergence were determined.
While the Balcalı (11) and Ceyhan (4) populations were close to the Iran
specimen, Erzin (5) was close to the Serbian and other Iran specimens. The specimens
of Cucumis melo L. from Seyhan, Solanum
nigrum L. from Gülek (Pozantı), Citrullus
lanatus (Tunb.) from Saimbeyli, S. nigrum from Ceyhan, and Vicia sativa L. from Balcalı were
digested with SacI, HpyCH4III, EcoP151, and both EcoP151 and
AccI by PCR-RFLP, respectively. Specific genetic diversity related to
altitude, host aphid and plant species was not determined.

Keywords

Genetic diversity, Braconidae, PCR-RFLP, biological control

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