Yıl 2018, Cilt 9 , Sayı 2, Sayfalar 124 - 136 2018-12-03

Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations
Bazı Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae: Aphidiinae) popülasyonlarında genetik çeşitlilik

Gül Satar [1] , Petek Toklu [2]


Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae: Aphidiinae) is an important aphid parasitoid that has a wide host range. Genetic diversity in L. confusus was studied by the PCR-RFLP method in terms of host aphid, host plant, and altitude in Adana and Hatay Provinces, Turkey. Restriction sites of SacI, AccI, HpyCH4III, and EcoP151 enzymes on the 709 bp mitochondrial COI gene region was detected by PCR-RFLP method. The sequences were compared with specimens from other countries (Serbia, China, France and Iran) and their net divergence were determined. While the Balcalı (11) and Ceyhan (4) populations were close to the Iran specimen, Erzin (5) was close to the Serbian and other Iran specimens. The specimens of Cucumis melo L. from Seyhan, Solanum nigrum L. from Gülek (Pozantı), Citrullus lanatus (Tunb.) from Saimbeyli, S. nigrum from Ceyhan, and Vicia sativa L. from Balcalı were digested with SacI, HpyCH4III, EcoP151, and both EcoP151 and AccI by PCR-RFLP, respectively. Specific genetic diversity related to altitude, host aphid and plant species was not determined.


Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae: Aphidiinae), geniş bir konukçu dizisine sahip önemli bir yaprak biti parazitoididir. L. confusus'taki genetik çeşitlilik, Adana ve Hatay illerinde konukçu yaprak biti, konukçu bitki ve yükseklik açısından PCR-RFLP yöntemi ile incelenmiştir. PCR-RFLP yöntemi ile SacI, AccI, HpyCH4III ve EcoP151 enzimlerinin 709 bp mitokondriyal COI gen bölgesinde enzim kesim noktaları saptanmıştır. Gen dizileri diğer ülkelerden (Sırbistan, Çin, Fransa ve İran) olan örnekler karşılaştırıldı ve net sapmaları belirlendi. Balcalı (11) ve Ceyhan (4) örnekleri İran örneğine yakınken, Erzin (5) Sırp ve diğer İran örneğine yakın olarak bulundu. Seyhan'dan Cucumis melo L, Gülek (Pozantı) den Solanum nigrum L., Saimbeyli'den Citrullus lanatus (Tunb.), Ceyhan'dan S. nigrum ve Balcalı'dan Vicia sativa L. örnekleri, PCR-RFLP ile sırasıyla SacI, HpyCH4III, EcoP151 ve EcoP151 ile AccI enzimleri tarafından sırayla kesildiği belirlendi yükseklik, konukçu afit ve bitki türlerine bağlı spesifik genetik çeşitlilik saptanmadı.


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Birincil Dil en
Konular Mühendislik, Ziraat
Bölüm Makaleler
Yazarlar

Yazar: Gül Satar (Sorumlu Yazar)
Kurum: Cukurova University Biotechnology Research and Application Center
Ülke: Turkey


Yazar: Petek Toklu
Kurum: Cukurova University Biotechnology Research and Application Center
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 3 Aralık 2018

Bibtex @araştırma makalesi { tbmd478059, journal = {Türkiye Biyolojik Mücadele Dergisi}, issn = {2146-0035}, eissn = {2548-1002}, address = {Çukurova Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü 01330 Sarıçam(Balcalı)-Adana/Türkiye}, publisher = {Türkiye Biyolojik Mücadele Derneği}, year = {2018}, volume = {9}, pages = {124 - 136}, doi = {10.31019/tbmd.478059}, title = {Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations}, key = {cite}, author = {Satar, Gül and Toklu, Petek} }
APA Satar, G , Toklu, P . (2018). Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations. Türkiye Biyolojik Mücadele Dergisi , 9 (2) , 124-136 . DOI: 10.31019/tbmd.478059
MLA Satar, G , Toklu, P . "Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations". Türkiye Biyolojik Mücadele Dergisi 9 (2018 ): 124-136 <https://dergipark.org.tr/tr/pub/tbmd/issue/40755/478059>
Chicago Satar, G , Toklu, P . "Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations". Türkiye Biyolojik Mücadele Dergisi 9 (2018 ): 124-136
RIS TY - JOUR T1 - Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations AU - Gül Satar , Petek Toklu Y1 - 2018 PY - 2018 N1 - doi: 10.31019/tbmd.478059 DO - 10.31019/tbmd.478059 T2 - Türkiye Biyolojik Mücadele Dergisi JF - Journal JO - JOR SP - 124 EP - 136 VL - 9 IS - 2 SN - 2146-0035-2548-1002 M3 - doi: 10.31019/tbmd.478059 UR - https://doi.org/10.31019/tbmd.478059 Y2 - 2018 ER -
EndNote %0 Türkiye Biyolojik Mücadele Dergisi Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations %A Gül Satar , Petek Toklu %T Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations %D 2018 %J Türkiye Biyolojik Mücadele Dergisi %P 2146-0035-2548-1002 %V 9 %N 2 %R doi: 10.31019/tbmd.478059 %U 10.31019/tbmd.478059
ISNAD Satar, Gül , Toklu, Petek . "Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations". Türkiye Biyolojik Mücadele Dergisi 9 / 2 (Aralık 2018): 124-136 . https://doi.org/10.31019/tbmd.478059
AMA Satar G , Toklu P . Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations. Türk. biyo. müc. derg. 2018; 9(2): 124-136.
Vancouver Satar G , Toklu P . Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations. Türkiye Biyolojik Mücadele Dergisi. 2018; 9(2): 136-124.