Detection of Viruses Infecting Sweet Cherry Cultivars and Elites at the Institute of Agriculture-Kyustendil, Bulgaria
Abstract
То аssess the virus infections of new and commercial sweet cherry cultivars and elites, surveys were carried out in two collection orchards at the Institute of Agriculture – Kyustendil located in the Southwest Bulgaria. In the spring of 2012 and 2013 a total of 165 samples were individually collected and tested by DAS-ELISA for Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), Plum pox virus (PPV), Apple chlorotic leafspot virus (ACLSV), Cherry leaf roll virus (CLRV) and Raspberry ringspot virus (RpRSV). A total of 24.2 % of ELISA-tested samples was infected at least by single virus. PDV (13.9 %) was the most prevalent followed by ACLSV (5.4 %) and PNRSV (4.8 %). The other viruses were no presented. The infection was found in both cultivars and elites. These results demonstrated that measures should be taken to avoid using infected parents for crossing
Keywords
References
- Anonymous, 2014. Fruit production in 2013. Bulletin № 267, Agro statistics. Ministry of Agriculture and Food -Sofia
- Amari, K.; Sanchez-Pina, M. A. and Pallas,V., 2004. Vertical transmission of Prunus necrotic ringspot virus by gametes in apricot. Acta Horticulturae 657: 109-113.
- Bassi D., Martelli G.P. 2003.Correlation between breeding techniques and gamic transmission of viruses in fruit tree crops. In : Myrta A. (ed.), Di Terlizzi B. (ed.), Savino V. (ed.). Virus and virus-like diseases of stone fruits, with particular reference to the Mediterranean region. Bari : CIHEAM, 2003. p. 9-13. (Options Méditerranéennes : Série B. Etudes et Recherches; n. 45).
- Borisova A.,Kamenova I.,Borovinova M., 2013. Viral and fungal diseases of stone fruit species in Kyustendil region. Journal of Mountain Agriculture on the Balkans, vol. 16, 1, 164-182.
- Clark, M.F. and Adams, A.M. 1977. Characteristics of the micoplate method of enzyme-linked immunosorbent detection of plant viruses. J.Gen. Virol.34: 475- 481 (ELISA) for the
- Crescenz A., Nuzzaci M., Levy L., Piazzolla P. and Hadidi A. 1995. Plum pox virus in sweet cherry. Acta Hort. 386: 219-225.
- Desvignes J.C. and Boye R., 1989. Different diseases caused by the chlorotic leafspot virus on the fruit trees. Acta Horticulture 235: 31-38
- Desvignes, J.C., 1999. Virus Diseases of Fruit Trees. Ctif. Ed., France.
- Flegg, C.L. and Clark, M.F. 1979. The detection of apple chlorotic leafspot virus by a modified procedure of enzyme – linked immunosorbent assay. Ann. Appl. Biol. 91: 61-65
- Jakubczyk, H., Lata, B., Sadowski, A. and Whitehead,
Detection of Viruses Infecting Sweet Cherry Cultivars and Elites at the Institute of Agriculture-Kyustendil, Bulgaria
Abstract
Keywords
References
- Anonymous, 2014. Fruit production in 2013. Bulletin № 267, Agro statistics. Ministry of Agriculture and Food -Sofia
- Amari, K.; Sanchez-Pina, M. A. and Pallas,V., 2004. Vertical transmission of Prunus necrotic ringspot virus by gametes in apricot. Acta Horticulturae 657: 109-113.
- Bassi D., Martelli G.P. 2003.Correlation between breeding techniques and gamic transmission of viruses in fruit tree crops. In : Myrta A. (ed.), Di Terlizzi B. (ed.), Savino V. (ed.). Virus and virus-like diseases of stone fruits, with particular reference to the Mediterranean region. Bari : CIHEAM, 2003. p. 9-13. (Options Méditerranéennes : Série B. Etudes et Recherches; n. 45).
- Borisova A.,Kamenova I.,Borovinova M., 2013. Viral and fungal diseases of stone fruit species in Kyustendil region. Journal of Mountain Agriculture on the Balkans, vol. 16, 1, 164-182.
- Clark, M.F. and Adams, A.M. 1977. Characteristics of the micoplate method of enzyme-linked immunosorbent detection of plant viruses. J.Gen. Virol.34: 475- 481 (ELISA) for the
- Crescenz A., Nuzzaci M., Levy L., Piazzolla P. and Hadidi A. 1995. Plum pox virus in sweet cherry. Acta Hort. 386: 219-225.
- Desvignes J.C. and Boye R., 1989. Different diseases caused by the chlorotic leafspot virus on the fruit trees. Acta Horticulture 235: 31-38
- Desvignes, J.C., 1999. Virus Diseases of Fruit Trees. Ctif. Ed., France.
- Flegg, C.L. and Clark, M.F. 1979. The detection of apple chlorotic leafspot virus by a modified procedure of enzyme – linked immunosorbent assay. Ann. Appl. Biol. 91: 61-65
- Jakubczyk, H., Lata, B., Sadowski, A. and Whitehead,
There are 10 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | March 1, 2014 |
| Submission Date | January 26, 2015 |
| Published in Issue | Year 2014 Volume: 1 Issue: Özel Sayı-1 |
