Tescilli Makarnalık Buğday (Triticum durum) Çeşitlerinin Sarı Pas (Puccinia striiformis f. sp. tritici) Hastalığına Karşı Dayanıklılığının Belirlenmesi

Abstract

Puccinia striiformis f. sp. tritici (Pst)’nin neden olduğu sarı pas hastalığı, buğday bitkisinin en yıkıcı hastalığı olup, bu hastalık ile mücadelede en etkili kontrol yöntemi dayanıklı bitki kullanılmasıdır. Bunun için yaygın olarak yetiştirilen çeşitlerin mevcut hastalık popülasyonuna karşı veya virülensliği yüksek olan Pst ırk/ırklarına karşı test edilmesi gerekmektedir. Çalışma kapsamında, Türkiye'de tescilli 54 adet makarnalık buğday çeşidinde doğal şartlarda gerçekleşen sarı pas enfeksiyonu değerlendirilmiştir. Çalışma, 2017 ve 2018 yıllarında Akdeniz Üniversitesi yerleşkesinde iki tekerrür olarak gerçekleştirilmiştir. Hastalık değerlendirmesinde “modifiye cobb” skalası kullanılmış ve ardından belirlenen hastalık reaksiyona göre enfeksiyon katsayısı hesaplanmıştır. Çalışmadan elde edilen sonuçlara göre, her iki yılda da çeşitlerin çoğunun sarı pas enfeksiyonuna orta derecede hassas olduğu belirlenmiştir. İlave olarak, Kızıltan 91, Altın 40/98, Yılmaz 98, İmren, Çeşit 1252 ve Kunduru 1149 makarnalık buğday çeşitleri mevcut hastalık popülasyonuna karşı tarla koşullarında dayanıklılık reaksiyonu göstermiştir. Yürütülecek ileri ki çalışmalarda bu çeşitlerde bulunan direnç geni veya genlerinin moleküler yöntemlerle belirlenmesi gerekmektedir. Ayrıca, Türkiye’de tespit edilen sarı pas ırklarına karşı ıslah programlarında geliştirilen yeni çeşitlerin test edilmesi hastalığın kontrolü açısından önemlidir.

Keywords

• Makarnalık buğday
• sarı pas
• Puccinia striiformis f. sp. tritici
• doğal enfeksiyon
• dayanıklılık

Assessment of Registered Durum Wheat (Triticum durum) Cultivars for Resistance to Yellow Rust (Puccinia striiformis f. sp. tritici) Disease

Abstract

Yellow rust disease caused by Puccinia striiformis f. sp. tritici (Pst) is the most destructive disease of wheat and the most effective control method against the disease is the use of resistant plants. For this reason, the cultivars commonly grown should be tested against the current disease population or Pst race/races of high virulence. In this study, the infection of yellow rust on 54 durum wheat cultivars registered in Türkiye was evaluated under natural conditions. This study was carried out with two replicates on the campus of Akdeniz University during the 2017-2018 years. For the disease assessment, the modified Cobb scale was used and the infection of the coefficient was calculated according to the disease reaction determined. Based on the results of this study, it was found that most of the varieties were moderately susceptible and susceptible to the natural yellow rust infection in both years. Additionally, Kızıltan 91, Altın 40/98, Yılmaz 98, İmren, Çeşit 1252, and Kunduru 1149 varieties were resistant to the current disease population under field conditions. In further studies, it is necessary to determine the resistance gene or genes found in these cultivars by molecular methods. Additionally, it is important that new varieties developed in breeding programs were analyzed for Pst races identified in our country for the control of the disease.

Keywords

• Durum wheat
• yellow rust
• Puccinia striiformis f. sp. tritici
• natural infection
• resistance

References

1. Afshari, F., 2013. Race analysis of Puccinia striiformis f. sp. tritici in Iran. Archives of Phytopathology and Plant Protection, 46(15): 1785-1796.
2. Agrios, G.N., 2004. Plant Pathology. Academic Press, New York.
3. Ali, S., Rahman, H., Shah, S.J.A., Shah, S.M., 2009. Assessment of field resistance using host-pathogen interaction phenotype for wheat yellow rust. African Crop Science Journal, 17(4): 213-221.
4. Anonim, 2021. Bitkisel Üretim İstatistikleri. Türkiye İstatistik Kurumu, (https://data.tuik.gov.tr/Bulten/ Index?p=Bitkisel-Uretim-Istatistikleri-2020-33737), (Erişim tarihi: 19.12.2021).
5. Anonymous, 2021. Crops and Livestock Products. Food and Agriculture Organization of the United Nations, (https://www.fao.org/faostat/en/#data/QCL), (Erişim tarihi: 19.12.2021).
6. Bahri, B., Shah, S.J.A., Hussain, S., Leconte, M., Enjalbert, J., de Vallavieille-Pope, C., 2011. Genetic diversity of the wheat yellow rust population in Pakistan and its relationship with host resistance. Plant Pathology, 60(4): 649-660.
7. Braun, H.J., Atlin, G., Payne, T., 2010. Multi location testing as a tool to identify plant response to global climate change. In: M.P., Reynolds (Ed.), Climate Change and Crop Production, CABI, Oxfordshire, UK, pp. 115-138.
8. Braun, H.J., Saari, E.E., 1992. An assessment of the potential of Puccinia striiformis f. sp. tritici to cause yield losses in wheat on the Anatolian plateau of Turkey. Vortrage fur Planzenzuchhtg, 24(8): 121-123.

9. Cat, A., Tekin, M., Akan, K., Akar, T., Catal, M., 2021. Races of Puccinia striiformis f. sp. tritici identified from the coastal areas of Turkey. Canadian Journal of Plant Pathology, 43(Sup2): 323-332.
10. Chen, W.Q., Wu, L.R., Liu, T.G., Xu, S.C., Jin, S.L., Peng, Y.L., Wang, B.T., 2009. Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Disease, 93(11): 1093-1101.
11. Chen, X.M., 2005. Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat. Canadian Journal of Plant Pathology, 27(3): 314-337.
12. Çetin, L., Düşünceli, F., Albustan, S., Bolat, N., Yıldırım, A.F., Hekimhan, H., Camcı, H., Ekiz, H., 2000. 1995-1998 yılları arasında Orta Anadolu buğday alanlarında sarı pas (Puccinia striiformis) virulanslarının dört lokasyonda kapan norserileriyle belirlenmesi. Orta Anadolu'da Hububat Tarımının Sorunları ve Çözüm Yolları Sempozyumu, 8-11 Haziran, Konya, s. 414-417.
13. de Vallavieille-Pope, C., Ali, S., Leconte, M., Enjalbert, J., Delosand, M., Rouzet, J., 2012. Virulence dynamics and regional structuring of Puccinia striiformis f. sp. tritici in France between 1984 and 2009. Plant Disease, 96(1): 131-140.
14. Gad, M.A., Li, H., Alam, M.A., Sajjad, M., Li, M., 2019. Geographical distribution and virulence phenotypes of Puccinia striiformis f. sp. tritici from wheat in Yunnan, China. ScienceAsia, 45(6): 572-580.
15. Gebreslasie, Z.S., Huang, S., Zhan, G.M., Badebo, A., Zeng, Q.D., Wu, J.H., Wang, Q.L., Liu, S. J., Huang, L.L., Wang, X.J., Kang, Z.S., Han, D.J., 2020. Stripe rust resistance genes in a set of Ethiopian bread wheat cultivars and breeding lines. Euphytica, 216(2): 1-14.
16. Ghanbarnia, K, Gourlie, R., Amundsen, E., Aboukhaddour, R., 2021. The changing virulence of stripe rust in Canada from 1984 to 2017. Phytopathology, 111(10): 1840-1850.
17. Hovmøller, M.S., Patpour, M., Rodriguez-Algaba, J., Thach, T., Justesen, A.F., Hansen, J.G., 2020. Global Rust Reference Center (GRRC) Annual Report 2019: Stem- and Yellow Rust Genotyping and Race Analyses. (https://agro.au.dk/fileadmin/www.grcc. au.dk/International_Services/Pathotype_YR_results/GRRC_annual_report_2019.pdf), Aarhus University, Denmark, (Erişim tarihi: 18.11.2021).
18. Iqbal, A., Khan, M.R., Ismail, M., Khan, S., Jalal, A., Imtiaz, M., Ali, S., 2020. Molecular and field-based characterization of yellow rust resistance in exotic wheat germplasm. Pakistan Journal of Agricultural Sciences, 57(6): 1457-1467.
19. Kasa, D., Negash, T., 2021. Evaluation of ethiopian wheat germplasm against yellow rust (Puccinia striiformis) disease under field condition. Agriculture, Forestry and Fisheries, 10(2): 61-65.
20. Li, Q., Wang, B., Chao, K., Guo, J., Song, J., Yue, W., Li, Q., 2016. Molecular detection of stripe rust resistance gene (s) in 115 wheat cultivars (lines) from the Yellow and Huai River valley wheat region. Journal of Phytopathology, 164(11-12): 946-958.
21. Mamluk, O.F., Cetin, L., Braun, H.J., Bolat, N., Bertschinger, L., Makkouk, K.M., Yildirim, A.F., Saari, E.E., Zencirci, N., Albustan, S., Cali, S., Beniwal, S.S., Dusunceli, F., 1997. Current status of wheat and barley diseases of Central Anatolia Plateau of Turkey. Phytopathologia Mediterranea, 36(3): 167-181.
22. Mert, Z., Düşünceli, F., Akan, K., Cetin, L., Yazar, S., Bolat, N., Yorgancılar, A., Ünsal, R., Ercan, B., Özseven, I., Demir, L., Dinçer, N., Ay, H., Tekdal, S., Kılıç, H., Bayramoğlu, H., Sermet, C., Öztürk, I., Tülek, A., Küçüközdemir, U., İlkhan, A., 2012. An overview of the network for important cereal diseases management research in Turkey between 2003 and 2011. The Proceedings of 13th International Cereal Rusts and Powdery Mildews Conference, 28 August-01 September, Beijing, China, pp. 208-209.
23. Mert, Z., Nazari, K., Karagoz, E., Akan, K., Ozturk, I., Tulek, A., 2016. First incursion of the warrior race of wheat stripe rust (Puccinia striiformis f. sp. tritici) to Turkey in 2014. Plant Diseases, 100(2): 528.
24. Nagarajan, S., 1986. Race 13 (67S8) of Puccinia striiformis virulent on Triticum spelta var. album in India. Plant Disease, 70(2): 173.
25. Pathan, A.K., Park, R.F., 2006. Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars. Euphytica, 149(3): 327-342.
26. Perronne, R., Diguet, S., de Vallavieille-Pope, C., Leconte, M., Enjalbert, J., 2017. A framework to characterize the commercial life cycle of crop varieties: Application to the case study of the influence of yellow rust epidemics on French bread wheat varieties. Field Crop Research, 209: 159-167.
27. Peterson, R.F., Campbell, A.B., Hannah, A.E., 1948. A diagrammatic scale for rust intensity on leaves and stems of cereals. Canadian Journal of Research, 26(5): 496-500.
28. Sandhu, R., Singh, B., Dey, T., Pandey, M.K., 2021. Assessment of some Indian wheat genotypes and breeding lines for adult plant resistance (APR) against stripe rust. International Journal of Chemical Studies, 9(2): 31-35.
29. Shewaye, Y., Mohammed, H., 2021. Screening and evaluation of bread wheat (Triticum aestivum L.) genotypes resistance to stripe rust. African Journal of Agricultural Research, 17(5): 766-779.
30. Sun, Q., Wei, Y., Ni, Z., Xie, C., Yang, T., 2002. Microsatellite marker for yellow rust resistance gene Yr5 in wheat introgressed from spelt wheat. Plant Breeding, 121(6): 539-541.
31. Tabassum, S., Ashraf, M., Chen, X., 2010. Evaluation of Pakistan wheat germplasms for stripe rust resistance using molecular markers. Science. China Life Sciences, 53(9): 1123-1134.
32. Tekin, M., Cat, A., Akan, K., Akar, T., Catal, M., 2021. A new virulent race of wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) on the resistance gene Yr5 in Turkey. Plant Disease, 105(10): 3292.
33. Wan, A.M., Chen, X.M., 2014. Virulence characterization of Puccinia striiformis f. sp. tritici using a new set of Yr single-gene line differentials in the United States in 2010. Plant Disease, 98(11): 1534-1542.
34. Wan, A.M., Chen, X.M., Yuen, J., 2016. Races of Puccinia striiformis f. sp. tritici in the United States in 2011 and 2012 and comparison with races in 2010. Plant Disease, 100(5): 966-975.
35. Wellings, C.R., McIntosh, R.A., 1990. Puccinia striiformis f. sp. tritici in Australasia: pathogenic changes during the first 10 years. Plant Pathology, 39(2): 316-325.
36. Zeng, Q.D., Han, D.J., Wang, Q.L., Yuan, F.P., Wu, J.H., Zhang, L., Wang, X.J., Huang, L.L., Chen, X.M., Kang, Z.S., 2014. Stripe rust resistance and genes in Chinese wheat cultivars and breeding lines. Euphytica, 196(2): 271-284.
37. Zeybek, A., Yiğit, F., 2004. Determination of virulence genes frequencies in wheat stripe rust (Puccinia striiformis f. sp. tritici) populations during natural epidemics in the regions of Southern Aegean and Western Mediterranean in Turkey. Pakistan Journal of Biological Sciences, 7(11): 1967-1971.
38. Zhan, G., Chen, X., Kang, Z., Huang, L., Wang, M., Wan, A., Cheng, P., Cao, S., Jin, S., 2012. Comparative virulence phenotypes and molecular genotypes of Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen in China and the United States. Fungal Biology, 116(6): 643-653.
39. Zhang, G.S., Zhao, Y.Y., Kang, Z.S., Zhao, J., 2020. First report of a Puccinia striiformis f. sp. tritici race virulent to wheat stripe rust resistance gene Yr5 in China. Plant Disease, 104(1): 284.
40. Zheng, S.G., Li, Y.F., Lu, L., Liu, Z., Zhang, C., Ao, D.H., Li, L.R., Zhang, C.Y., Liu, R., Luo, C.P., Wu, Y., Zhang, L., 2017. Evaluating the contribution of Yr genes to stripe rust resistance breeding through marker-assisted detection in wheat. Euphytica, 213(2): 1-16.