Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts

Year 2018, , 253 - 259, 31.10.2018

Nuraniye Eruygur Esra Uçar

https://doi.org/10.19159/tutad.425732

Cited By: 2

Abstract

In this study, inhibition ability of Veronica officinalis extracts against
Alzheimer’s disease-related enzymes
acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), diabetes
mellitus related enzymes α-glucosidase and α-amylase and antioxidant of Veronica
officinalis were investigated. To the best of our knowledge, there are no
previous studies on the enzyme inhibition activities of the V. officinalis
extracts. For this aim, V. officinalis
extracted with methanol and water by maceration method and their antioxidant
activities were evaluated by DPPH (2,2-Diphenyl-1-picrylhydrazyl), ABTS [2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging
assays, total phenol, total flavonoid content, iron chelating and ferric
reducing power assays. The enzyme inhibition activity was performed with
96-well plate method. According to the obtained results; the plant extracts
have good antioxidant effects. In the same time, the water extract have
stronger inhibition activity against AChE, BuChE and α-glucosidase, α-amylase,
and tyrosinase than methanol extract. The experimental findings indicated that
the water extract exerted the higher α-glucosidase, α-amylase and tyrosinase
(82.07 ± 2.91, 63.61 ± 3.93 and 47.47 ± 0.53 at 2 mg mL^-1,
respectively) inhibition than reference drugs. The obtained results demonstrate
that this plant has a significant potential for improving pharmaceutical
formulations. 

Keywords

Antioxidant, alzheimer’s, diabetes, tyrosinase, Veronica officinalis

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