Farklı dezenfektan solüsyonlarının bal arıları (Apis mellifera )üzerindeki etkilerinin araştırılması

Year 2021, Volume: 92 Issue: 2, 143 - 151, 15.06.2021

Sedat Sevin , Ahmet Ceylan , Özge Özgenç , Gökhan Akdeniz , Fatih Yılmaz , Dilek Kabakcı , Ender Yarsan

https://doi.org/10.33188/vetheder.852336

Cited By: 1

Abstract

Bu çalışmada arıcılıkta kullanılan biyosidal ve nano gümüş içerikli dezenfektanların arılar üzerine toksik etkilerinin araştırılması amaçlanmıştır. Kovanların dezenfeksiyonunda kullanılan biyosidal içerikli ve nano gümüş içerikli preparatlar ticari firmalardan temin edildi. In vivo denemeler için 3 grup oluşturuldu. Kontrol grubuna (Grup 1; n=10) sadece şurup (1/1 oranında sakkaroz-su) verildi. Deneme gruplarından birine biyosidal içerikli preparat (Grup 2; n=10) ve diğerine nano gümüş içerikli preparat (Grup 3; n=10) oral yolla arı başına 2 µl olacak şekilde otomatik pipet ile verildi. Uygulamadan 24 saat sonra tüm gruplarda ölen arılar sayıldı ve histomorfolojik analizler için gruplarda canlı kalan arıların mideleri alındı. Saha denemeleri Ordu Arıcılık Enstitüsü Müdürlüğü arılığında gerçekleştirildi. Herhangi bir uygulama yapılmayan kontrol grubu (Grup 1) ile birlikte biyosidal içerikli (Grup 2) ve nano gümüş içerikli (Grup 3) preparatların uygulandığı iki uygulama grubu oluşturuldu. Preparatlar gruplara püskütme şeklinde uygulandı ve arı ölümleri kaydedildi. Saha koşullarında kovanlara uygulanan iki dezenfektan, kontrol grubuna göre daha çok arı ölümlerine sebep oldu. En fazla arı ölümleri Nano gümüş içerikli dezenfektanın uygulandığı kovanlarda görüldü. Laboratuar denemelerinde nano gümüş içerikli preparatın yüksek oranda arı ölümlerine ve histomorfolojik incelemelerde mide epitelinde ciddi hasarlara neden olduğu belirlendi. Çalışma sonuçları, dezenfektan maddelerin arılar üzerine doğrudan uygulanmasının kolonide ciddi ölümlere neden olduğunu göstermiştir. Biyosidal ve kimyasal tabanlı preparatlar ile yapılacak kovan dezenfeksiyonu, arıların bulunmadığı dönemde gerçekleştirilmelidir

Keywords

Arı, Arı sağlığı, dezenfektan, nano gümüş, biyosidal

Investigation of the effects of different disinfectant solutions on honey bees (Apis mellifera )

Abstract

In this study, it was aimed to investigate the toxic effects of biocidal and nano silver-containing disinfectants, which were used in beekeeping, on bees. Biocidal and nano-silver-containing preparations used in disinfection of hives were obtained from commercial companies. Syrup (1/1 sucrose-water) was given to the control group (Group 1; n = 10). Biocidal preparation (Group 2; n = 10) and nano-silver containing preparation (Group 3; n = 10) were given to one of the experimental groups via an automatic pipette, orally 2 µl per bee. 24 hours after the application, the bees that died in all groups were counted and the midgut tissues of the bees that survived in the groups were taken for histomorphological analysis. No application was performed in the control group (Group 1). Different disinfection solution was used in the group 2 (biocidal ingredient) and Group 3 (nano silver contents). The preparations were applied to the groups by spraying and bee deaths were recorded. Two disinfectants applied to the hives under field conditions, were found to cause more bee deaths than the control group. The highest bee death was in the nano silver group. In laboratory trials, the nano-silver-containing preparation was observed to cause high number of bee deaths and serious damage to the midgut epithelium in histomorphological examinations. The results of the study showed that direct application of disinfectant substances on bees caused serious deaths in the colony. Biocidal and chemical based preparations and hive disinfection should be applied in the empty beehives.

Keywords

Bee, Bee health, Disinfectant, Nano silver, Biocidal

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