Patateste (Solanum tuberosum L.) in vitro Mikrotuberizasyon Üzerine Fotoperiyot, Jasmonik Asit ve Aktif Kömürün Etkileri

Year 2017, Volume: 27 Issue: 3, 318 - 325, 29.09.2017

Seray Kenar Gökçen Baysal Furtana Şeküre Şebnem Ellialtıoğlu Rukiye Tıpırdamaz

https://doi.org/10.29133/yyutbd.285085

Abstract

Bu çalışmada, ekonomik değeri olan Solanum tuberosum L. cv. Marfona’da in vitro mikrotuber oluşumu üzerinde fotoperiyot ile ortama ilave edilen Jasmonik asit (JA), aktif kömür (AC) ve agarın etkileri araştırılmıştır. Bu amaçla çalışma 4 aşamada gerçekleştirilmiştir: Tuberlerden sürgün oluşumu, in vitro sürgün ucu kültürü, mikroçoğaltım ve mikrotuberizasyon. Sürgün ucu kültürü ile in vitro çoğaltılan bitkilerden alınan tek boğum eksplantları JA (0.0-0.2 mg L-1), agar (%0.5, %0.7) ve AC (0, %0.2) içeren çift fazlı Murashige and Skoog (MS) ortamlarında 2 farklı fotoperiyotta kısa gün (8/16 saat aydınlık/karanlık veya sürekli karanlık) kültüre alınmıştır. En yüksek mikrotuber verimi (%137) ve en yüksek ortalama mikrotuber ağırlığı (183 mg); % 0.2 AC, %0.5 agar içeren çift fazlı MS ortamında ve karanlıkta elde edilmiştir. AC’nin mikrotuber oluşumu üzerindeki olumlu etkisinin JA’den daha fazla olduğu ve AC’nin karanlıkta daha etkili olduğu belirlenmiştir.

Keywords

Patates (Solanum tuberosum L.), in vitro Mikrotuberizasyon, Çift fazlı ortam, Jasmonik Asit, Aktif Kömür

The Effects of Photoperiod, Jasmonic Acid and Activated Charcoal on in vitro microtuberization in Potatoes (Solanum tuberosum L.)

Abstract

In this study, effects of added agar, jasmonic acid (JA), activate charcoal (AC) with photoperiod regime on in vitro microtuber formation in Solanum tuberosum cv. Marfona. Study has actualized as 4 main steps; shoot formation of the tubers of S. tuberosum, in vitro shoot-tip culture, micropropagation and microtuberization. The effects of JA (0.0-0.2 mgL-1), agar (0.5% or 0.7%), AC (0 and 0.2%) microtuberization were investigated. The highest microtuber yield (137%) and the highest microtuber average (183 mg/tuber) were obtained at the double-layer Murashige and Skoog (MS) medium which contains 0.2% AC, 0.5% agar under darkness conditions. It has been determined that positive effect of AC on microtuber formation is higher than JA. AC was found much more effective in the dark.

Keywords

Potato (Solanum tuberosum L.), in vitro, Microtuberization, Double-layer medium, Jasmonic acid, Activated charcoal

References

• Aslam A, Iqbal J (2010). Combined effect of cytokinin and sucrose on in vitro tuberization parameters of two cultivars, diamant and red norland of potato (Solanum Tuberosum). Pakistan Journal of Botany, 42 (2): 1093-1102.
• Beyazova S (1999). Production of microtubers in potato (Solanum tuberosum). Yüksek Lisans Tezi, Ortadoğu Teknik Üniversitesi, Ankara.
• Bhatia AK, Pandita ML, Khurana SC (1992). Plant growth substances and sprouting conditions-ıı. effect on tuber yield and multiplication rate in seed potato production. Journal of Indian Potato Association, 19: 154-156.
• Burrows GE, Tyrl RJ (2001). Toxic plants of North America. Iowa State University Press, 1342.
• Carrera E, Bou J, Garcia- Martinez JL, Prat S (2000). Changes in GA 20-oxidase gene expression strongly affect stem length, tuber induction and tuber yield of potato plants. The Plant Journal, 22(3); 247-256.
• Castro G, Guillermina A, Agüero C, Tizio R (2000). Interaction between jasmonic and gibberellic acids on in vitro microtuberization of potato plantlets. Potato Research, 43: 83-88.
• Chevre AM, Gill SS, Mouras A, Salesses G (1983). In vitro vegetative multiplication of chestnut. The Journal of Horticultural Science and Biotechnology, 58: 23-29.
• Garner N, Blake J (1989). The induction and development of potato microtubers in vitro on media free of growth regulating substances. Annals of Botany, 63: 663-674.
• Harmey MA, Ikuma H, Bonner WD (1996). Near ultra-violet spectrum of white potato mitochondria. Nature, 209: 174-175.
• Hoque ME (2010). In vitro tuberization in potato (Solanum tuberosum L.). Plant Omics Journal, 3(1): 7-11.
• Jackson SD, Willmitzer L (1994). Jasmonic acid spraying does not induce tuberisation in short-day-requiring potato sspecies kept in non-inducing conditions. Planta, 194(2): 155-159.
• Jackson SD (1999). Multiple signaling pathways control tuber induction in potato. Plant Physiology, 119: 1–8.
• Jasik J, Klerk GJ (2006). Effect of methyl jasmonate on morphology and dormancy development in lily bublets regenerated in vitro. Journal of Plant Growth Regulation, 25: 45-51.
• Jasik J, Mantell SH (2000). Effects of jasmonic acid and its methyl ester on in vitro microtuberization of three food yam (Dioscorea) species. Plant Cell Reports, 19: 863-867.
• Kenar S (2013). Patateste (Solanum tuberosum L.) in vitro mikrotuberizasyon üzerine jasmonik asit-giberellik asit etkileşiminin araştırlması. Yüksek Lisans Tezi, Hacettepe Üniversitesi, Ankara.
• Kenar S, Tıpırdamaz R (2012). Patateste (Solanum tuberosum L.) in vitro mikrotuberizasyon üzerine bazı faktörlerin etkilerinin araştırılması, Kırgızistan Birinci Uluslararası Biyoloji Kongresi, 24-26 Eylül, Bişkek, Kırgızistan.
• Khuri S, Moorby J (1995). Investigations in to the role of sucrose in potato cv. Estima microtuber production in vitro. Annals of Botany, 75: 295-303.
• Khuri S, Moorby J (1996). Nodal segments or microtubers as explants for in vitro microtuber production of potato. Plant Cell, Tissue and Organ Culture, 45: 215-222.
• Kim SK, Kim JT, Jang SW, Lee SC, Lee BH, Lee IJ (2005). Exogenous effect of gibberellins and jasmoate on tuber enlargement of Dioscorea opposite. Agron Resource, 3: 39-44.
• Kruskal WH, Wallis WA (1952). Use of ranks in one-criterion variance analysis. Journal of the American Statistical Association, 47(260): 583-621.
• Lajayer HM, Esmaielpour B, Chamani E (2011). Hinokitiol and activated charcoal ınfluence the microtuberization and growth of potato (Solanum tuberasum Cv. Agria) plantlets in vitro. Australian Journal of Crop Science, 5(11): 1481-1485.
• Martinez-Garcia JF, Garcia-Martinez JL, Bou J, Prat S (2002). The interaction of gibberellins and photoperiod in the control of potato tuberization. Plant Growth Regulation, 20: 377-386.
• Moeinil MJ, Armin M, Asgharipour MR, Yazdi SK (2011). Effects of different plant growth regulators and potting mixes on micro-propagation and mini-tuberization of potato plantlets, Advances in Environmental Biology, 5(4): 631-638.
• Nelson DL, Cox MM (2005). Lehninger Biyokimyanın İlkeleri, 3. Baskıdan çeviri, (çeviri: Kılıç N), Palme yayıncılık.
• Nistor A, Campeanu G, Atanasiu N, Chiru N, Karácsonyi D (2010). Influence of potato genotypes on “in vitro” production of microtubers. Romanian Biotechnological Letters, 15: 3.
• Öztürk G (2003). Patateste (Solanum tuberosum L.) in Vitro koşullarda mikro yumru üretimine farklı besin ortamlarının etkisi. Yüksek Lisans Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
• Phillips BJ, Hughes JA, Phillips JC, Walters DG, Anderson D, Tahourdin CSM (1996). A study of the toxic hazard that might be associated with the consumption of green potato tops. Food Chemistry and Toxicology, 34: 439–448.
• Pierik RLM (1987). In Vitro culture of higer plants. Martinus Nijhoff Publishers, Dordrect, 344.
• Pruski K, Duplessis P, Lewis T, Astatkie T, Nowak J (2001). Jasmonate effect on in vitro tuberization of potato (Solanum tuberosum L.) cultivars under light and dark conditions. Potato Research 44: 315-325.
• Pruski K, Duplessis P, Lewis T, Astatkie T, Nowak J, Struik PC (2002). Jasmonate effect on in vitro tuberization of potato (Solanum tuberosum L.) cultivars under light and dark conditions. Potato Research, 44: 315-325.
• Ranalli P (1997). Innovative propagation methods in seed tuber multiplication programmes. Potato Research, 40: 439-453.
• Rayirath UP, Lada RR, Caldwell CD, Asideu SK, Sibley KJ (2011). Role of ethylene and jasmonic acid on rhizome ınduction and growth in rhubarb (Rheum rhabarbaum L.). Plant Cell Tissue and Organ Culture, 105: 253-263.
• Romanov GA, Aksenova NP, Konstantinova TN, Golyanovskaya SA, Kossman J, Willmitzer L (2000). Effect of indole-3-acetic acid and kinetin on tuberisation parameters of different cultivars and transgenic lines of potato in vitro. Plant Growth Regulation, 32: 245-251.
• Sangwan RS, Detrez C, Sangwan-Norreel BS (1987). In vitro culture of shoot-tip meristems ın some hıgher plants. Symposium on In Vitro Problems Related to Mass Propagation of Horticultural Plants.
• Seabrook JEA, Colpman S, Levy D (1993). Effect of photoperiod on in vitro tuberization of potato (Solanum tuberosum L.). Plant Cell, Tissue and Organ Culture, 34(1): 43-51.
• Sokal R, Rohlf FJ (1995). Biometry, The Principles and Practice of Statistics in Biological Research, Third Edition, WH Freeman and Co, New York, USA.
• Taiz L, Zeiger E (2008). Bitki Fizyolojisi, 3. Baskıdan Çeviri (Çeviri editorü: Türkan İ), Palme Yayıncılık, Ankara.
• Takahashi K, Fujino K, Kikuta Y, Koda Y (1994). Expansion of potato cells in response to jasmonic acid. Plant Science, 100: 3-8.
• Tianyu Z, Junlian Z, Di W, Li W, Yansen C, Ximei D, Yuhui L, Youzhong L (2006). Study on the optimization of İnducing potato microtuber system in different varieties. Chinese Agricultural Science Bulletin, 10.
• Tican A, Chiru N, Ianosi M, Ivanovici DE, Sand C (2008). In Vitro culture and micro / mini tubers behavior of romanian potato varieties christian and roclas on microtubers production. 17th Triennial Conference Of European Assocation Fot Potato Research, July 6-10, Braşov, Romania.
• Vreugdenhil D, Xu X, Lammeren AAM (1998). Cell division and cell enlargement during potato tuber formation. Journal of Experimental Botany, 49: 320, 573–582.
• Zhang Z, Zhou W (2005). The role of GA, IAA and BAP in the regulation of in vitro shoot growth and microtuberization in potato. Acta Physiologiae Plantarum, 27(3B): 363-369.
• Zhang ZJ, Li HZ, Zhou WJ, Takeuchi Y, Yoneyama K (2006). Effect of 5-aminolevulinic acid on development and salt tolerance of potato (Solanum tuberosum L.) microtubers in vitro. Plant Growth Regulation, 49: 27–34.