Year 2019, Volume 44, Issue 2, Pages 309 - 317 2019-04-15

PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS
SOĞUK PRES FINDIK POSALARINDAN ÜRETİLMİŞ TRİPTİK PEPTİTLERİN IN VITRO ACE İNHİBE EDİCİ AKTİVİTELERİ ÜZERİNE ÖN INCELEMELER

İbrahim Gülseren [1] , Bilal Çakır [2]

56 144

Hazelnut (Corylus avellana L.) is a rich source of proteins which are concentrated in deoiled meals during hazelnut oil manufacture. Here, an in vitro attempt was made to evaluate the biological functionality of tryptic hazelnut peptides. In our in silico investigations, a specific subset of hazelnut proteins was predicted to demonstrate ACE-inhibitory and DPP-IV-inhibitory activities. Consequently, hazelnut protein concentrates were produced from cold press deoiled meals and subjected to tryptic hydrolysis (37°C). The progress of trypsinolysis was monitored using size exclusion chromatography (SEC), fast protein liquid chromatography (FPLC) and Raman spectroscopy. While the protein hydrolyzates had limited ACE-inhibitory activity, after 240 min of trypsinolysis, ACE-inhibitory activity was approximately 40% at a relatively low protein concentration level. Although further clarifications are necessary, current findings demonstrated hazelnut meal could be considered as a valuable source of ACE-inhibitory peptides that can be utilized in the formulation of functional foods and food supplements. 

Fındık (Corylus avellana L.) proteince zengin bir kaynaktır ve soğuk pres fındık yağı üretimi sürecinde oluşan fındık posaları proteince de zenginleşmektedir. Bu çalışmada, triptik fındık peptitlerinin biyolojik fonksiyonlarını değerlendirmek için in vitro analizlerde bulunulmuştur. Grubumuzun önceki çalışmalarında, in silico yöntemlerle fındık peptitlerinin biyoaktivitelerini incelemek için fındık proteinlerinin spesifik bir alt kümesi incelenmiş ve ACE- ve DPP-IV-önleyici aktivitelerin varlığı öngörülmüştür. Bu bulgulara bağlı olarak, soğuk pres yönteminde ortaya çıkan fındık posalarından, fındık proteini konsantreleri üretilmiş ve bu konsantreler triptik hidrolize (37°C) tabi tutulmuştur. Tripsinoliz prosesi, boyutsal ayrım kromatografisi (SEC), hızlı protein sıvı kromatografisi (FPLC) ve Raman spektroskopisi kullanılarak takip edilmiştir. Enzimatik muamele görmemiş protein konsantrelerinin sınırlı ACE inhibe edici aktivitesi varken, 240 dakikalık tripsinolizde oluşan peptitlerin ACE-inhibitör aktivitesi, düşük protein konsantrasyonlarında dahi yaklaşık %40 düzeyinde gerçekleşmiştir. Mevcut bulgular, fındık posasının ACE-inhibitör peptitler açısından önemli bir kaynak olarak değerlendirilebileceğini ve fındık posasından hidrolizatlarının fonksiyonel gıdaların ve gıda takviyelerinin formülasyonunda kullanılabileceğini göstermiştir.

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Primary Language en
Subjects Science
Published Date 2019
Journal Section Articles
Authors

Author: İbrahim Gülseren
Institution: İSTANBUL SABAHATTİN ZAİM ÜNİVERSİTESİ, MÜHENDİSLİK VE DOĞA BİLİMLERİ FAKÜLTESİ
Country: Turkey


Author: Bilal Çakır
Institution: İSTANBUL SABAHATTİN ZAİM ÜNİVERSİTESİ, MÜHENDİSLİK VE DOĞA BİLİMLERİ FAKÜLTESİ
Country: Turkey


Dates

Publication Date: April 15, 2019

Bibtex @research article { gida499453, journal = {GIDA}, issn = {1300-3070}, eissn = {1309-6273}, address = {Gıda Teknolojisi Derneği}, year = {2019}, volume = {44}, pages = {309 - 317}, doi = {10.15237/gida.GD18125}, title = {PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS}, key = {cite}, author = {Gülseren, İbrahim and Çakır, Bilal} }
APA Gülseren, İ , Çakır, B . (2019). PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS. GIDA, 44 (2), 309-317. DOI: 10.15237/gida.GD18125
MLA Gülseren, İ , Çakır, B . "PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS". GIDA 44 (2019): 309-317 <http://dergipark.org.tr/gida/issue/43567/499453>
Chicago Gülseren, İ , Çakır, B . "PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS". GIDA 44 (2019): 309-317
RIS TY - JOUR T1 - PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS AU - İbrahim Gülseren , Bilal Çakır Y1 - 2019 PY - 2019 N1 - doi: 10.15237/gida.GD18125 DO - 10.15237/gida.GD18125 T2 - GIDA JF - Journal JO - JOR SP - 309 EP - 317 VL - 44 IS - 2 SN - 1300-3070-1309-6273 M3 - doi: 10.15237/gida.GD18125 UR - https://doi.org/10.15237/gida.GD18125 Y2 - 2019 ER -
EndNote %0 THE JOURNAL OF FOOD PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS %A İbrahim Gülseren , Bilal Çakır %T PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS %D 2019 %J GIDA %P 1300-3070-1309-6273 %V 44 %N 2 %R doi: 10.15237/gida.GD18125 %U 10.15237/gida.GD18125
ISNAD Gülseren, İbrahim , Çakır, Bilal . "PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS". GIDA 44 / 2 (April 2019): 309-317. https://doi.org/10.15237/gida.GD18125
AMA Gülseren İ , Çakır B . PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS. GIDA. 2019; 44(2): 309-317.
Vancouver Gülseren İ , Çakır B . PRELIMINARY INVESTIGATIONS IN VITRO ACE-INHIBITORY ACTIVITIES OF TRYPTIC PEPTIDES PRODUCED FROM COLD PRESS DEOILED HAZELNUT MEALS. GIDA. 2019; 44(2): 317-309.