Effect of Short Multiwalled Carbon Nanotubes on Escherichia coli K-12 Strain

Yıl 2022, Cilt: 5 Sayı: 2, 43 - 48, 27.06.2022

Tahsin Ertaş Bircan Dinç

Öz

Objective: Multi-walled carbon nanotubes (MWNTs), obtained by the graphene structure forming multi-walled cylindrical structures, find wide usage areas in different areas with their large surface areas, hydrophobic structures, and high electron conductivity. Although their drug transport properties are evaluated, they are used in biosensors and in synthesizing antibacterial materials with polymers. There are many data on the toxicities of MWNTs with different physicochemical properties. In addition to these, evaluation of antibacterial effects has become very important in recent years. In this study, it was aimed to evaluate the antibacterial effect of MDNTs on E. coli K12 strain, which are shorter than most of the MWNTs in the literature, which we have previously applied to different cell lines, nematodes and which have a very limited toxic effect up to high concentrations.
Methods: Different concentrations ranging from 20 to 1000 µg/ml were prepared from MDNTs and applied to E. coli K12 strain. Colony count, bacterial optical density and microscopic images were evaluated.
Results: Compared with the control, it was observed that it affected the proliferation at every concentration from 10 µg/ml to 100 µg/ml, and the antibacterial effect was most pronounced at 50 and 100 µg/ml concentrations. There was no significant difference in inhibition dose between 10 and 20 µg/ml. Growth inhibition was observed to be greater than 20% after 90 minutes at high doses.
Conclusion: Bacteria grown in liquid and solid agar revealed different inhibition properties at low and high concentrations. Depending on the concentration, it was determined that the absorbance and viability values of E. coli cells decreased in both experiments. In addition, decreases in bacterial colonies, and in bacterial activity performed with DAPI staining and image analyzes are quite evident.

Anahtar Kelimeler

Multi-Walled Carbon Nanotube, E. coli, inhibition, density, antibacterial effect

Proje Numarası

37475

Kısa Çok Duvarlı Karbon Nanotüplerin Escherichia coli K-12 ‘ye Etkisi

Öz

Amaç: Grafen yapının iç içe silindirler oluşturması ile elde edilen çok duvarlı karbon nanotüpler (ÇDNT), geniş yüzey alanları, hidrofobik yapıları, yüksek elektron iletkenlikleri ile geniş kullanım alanı bulmaktadırlar. İlaç taşıma özellikleri değerlendirilmekle birlikte, biyosensörlerde ve polimerlerle birlikte antibakteriyel malzeme sentezlenmesinde kullanılmaktadırlar. Farklı fizikokimyasal özelliklere sahip ÇDNT’lerin toksisitelerine dair çok sayıda veri vardır. Bunların yanında son yıllarda antibakteriyel etkilerinin değerlendirilmesi oldukça önemli hale gelmiştir. Buradaki araştırmada daha önce farklı hücre soylarına ve yuvarlak solucanlara uyguladığımız; yüksek konsantrasyonlara kadar oldukça sınırlı bir toksik etkiye sahip olduğunu bildiğimiz, literatürde bulunan ÇDNT çeşitlerinin çoğuna göre daha kısa ÇDNT’ lerin, E. coli K12 üzerindeki anti bakteriyel etkisi değerlendirilmek istenmiştir.
Yöntem: ÇDNT’ lerden 20 - 100 µg/ml aralığında değişen farklı konsantrasyonlar hazırlanmış ve E. coli K12’ye uygulanmıştır. Koloni sayımı, bakteriyel konsantrasyon ve mikroskobik görüntüler değerlendirilmiştir.
Bulgular: Kontrol ile karşılaştırıldığında 10 µg/ml’den 100 µg/ml’ye, her konsantrasyonda ÇDNT’lerin bakterilerin çoğalmasını etkilediği, 50 ve 100 µg/ml konsantrasyonlarda en çok antibakteriyel etkinin ortaya çıktığı görülmüştür. 10 ve 20 µg/ml arasında, inhibisyon dozu olarak belirgin bir fark izlenmemiştir. Yüksek dozlarda 90. dakikadan sonra çoğalma inhibisyonunun %20'den fazla olduğu görülmüştür.
Sonuç: Sıvı ve katı besiyerinde çoğaltılan bakteriler düşük ve yüksek konsantrasyonlarda farklı inhibisyon özellikleri ortaya çıkarmıştır. Konsantrasyona bağlı olarak E. coli K-12’nin absorbans ve canlılık değerlerinde azalma olduğu her iki deneyde de tespit edilmiştir. Ayrıca DAPI boyama ile ÇDNT uygulanan bakteri aktivitesinde ve mikroskopta yapılan görüntü analizlerinde de, nanotüp miktarındaki artışa bağlı olarak bakteri kolonilerindeki azalmalar oldukça belirgindir.

Anahtar Kelimeler

Çok Duvarlı Karbon Nanotüp, E. coli, inhibisyon, yoğunluk, anti bakteriyel etki

Destekleyen Kurum

İstanbul Üniversitesi Bilimsel Araştırmalar Projeleri Birimi

Proje Numarası

37475

Teşekkür

Değerli katkılarından ötürü MSc. Recep ÜSTÜNSOY'a teşekkür ederiz.

Kaynakça

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