Optimal Concentration of Clove Oil in Anesthetization of Goldfish (Carassius auratus) and its Effect on Stocking Density for Fish Transportation

Öz

In this study, the effective concentrations of clove oil for deep anesthesia (30 min) and transportation (15 h) of goldfish (Carassius auratus) with an average weight of 4.16±0.61g were determined. Besides, the effect of clove oil on stocking density in transporting fish for 15 hours was determined. Depending on the concentrations and application time of clove oil, induction time ranged from 2 to 11 min while recovery time varied between 5 and 17 min. Depending on the concentrations and application time of clove oil, the respiratory rate of fish increased from 73/min to 104/min with the addition of clove oil in the anesthetic-free water. 6-12 µL/L for light anesthesia and 18-24 µL/L for deep anesthesia at a 30 min period, 6-9 µL/L for light anesthesia and 12-18 µL/L for deep anesthesia at a 15 h period are determined concentrations of clove oil that can be used safely. The toxic concentrations of clove oil were determined as 42 µL/L for 30 min application, 21 µL/L for 15 h application. Adding clove oil to the transport water did not affect the survival rate of the fish during the 15 h transport period. When transporting juvenile goldfish in 3/4 oxygen compressed nylon bags for 15 h, the most suitable stocking rate is 80 or 100 fish/L with a 98-99% survival rate.

Anahtar Kelimeler

• goldfish
• clove oil
• anesthesia
• stocking density
• transportation

Karanfil Yağının Japon Balığının (Carassius auratus) Anestezisinde Optimal Konsantrasyonu ve Balığın Taşınmasında Stoklama Yoğunluğuna Etkisi

Öz

Bu çalışmada, karanfil yağının ortalama ağırlığı 4,16±0,61 g olan japon balığının (Carassius auratus) derin anestezi (30 dk) ve taşınması (15 s) için etkili olan konsantrasyonları tespit edilmiştir. Ayrıca karanfil yağının balıkların 15 s süreli taşınmasında stoklama yoğunluğu üzerindeki etkisi belirlenmiştir. Karanfil yağının konsantrasyonu ve uygulama süresine bağlı olarak anesteziye giriş süresi 2 ile 11 dk, kendine gelme süresi ise 5 ile 17 dk arasında değişmiştir. Balıkların solunum sayısı anestezi içermeyen suda 73/dk iken, karanfil yağı uygulanmış olanlarda konsantrasyon ve uygulama süresine bağlı olarak 104/dk’ya kadar çıkmıştır. Karafil yağının 30 dk sürede hafif anestezi için 6-12 µL/L, derin anestezi için 18-24 µL/L; 15 s sürede hafif anestezi için 6-9 µL/L, derin anestezi için 12-18 µL/L konsantrasyonları güvenle kullanılabilir. Karanfil yağının toksik konsantrasyonları 30 dk uygulama için 42 µL/L, 15 s uygulama için 21 µL/L olarak saptanmıştır. Balıkların 15 s süreli taşınmasında taşıma suyuna karanfil yağı ilavesinin balığın yaşama oranı üzerine bir etkisi olmamıştır. Jüvenil japon balıklarının 3/4 oksijen basılmış naylon torbalarda 15 s süre ile taşınmasında, %98-99 yaşama oranı ile en uygun stoklama oranları 80 veya 100 adet/L’dir.

Anahtar Kelimeler

• japon balığı
• karanfil yağı
• anestezi
• stoklama yoğunluğu
• taşıma

Kaynakça

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