Farklı Zenginleştirici Emülsiyonların Rotifer (Brachionus plicatilis) Yığın Kültüründe Kullanımı

Yıl 2022, , 49 - 59, 01.03.2022

Hasan Batuhan Emre Özdoğan , Sevgi Savaş

https://doi.org/10.22392/actaquatr.950155

Cited By: 1

Öz

Bu çalışmada, rotifer Brachionus plicatilis kültüründe 2 formüle emülsiyon (Emülsiyon-I ve Emülsiyon-II) ve 3 farklı ticari ürün (Olio w-3, Red Pepper ve (n-3) Top Rich) kullanımının popülasyon artışı üzerine etkisi araştırılmıştır. Rotiferler için oluşturulan deneme grupları sırasıyla; DHA-Gold (Kontrol), DHA-Gold + Emülsiyon-I (1. Grup), DHA-Gold + Emülsiyon-II (2. Grup), DHA-Gold + Olio w-3 (3. Grup), DHA-Gold + Red Pepper (4. Grup), DHA-Gold + (n-3) Top Rich (5. Grup) olarak belirlenmiştir. Farklı besleme rejimine göre rotiferlerin populasyon artışının belirlenmesi için 1 L kültür hacminde yığın kültür, başlangıç yoğunluğu 100 birey/ml olacak şekilde 7 gün süre ile gerçekleştirilmiş ve günlük olarak birey sayımları yapılmıştır. Deneme sonunda rotiferlerde en yüksek birey sayısı, büyüme hızı ve fekondite oranı DHA-Gold + Emülsiyon-II ile zenginleştirilen grupta sırasıyla 1250 ± 11,54 birey/ml, 0,37 ± 0,00 bölünme/gün ve 0,38 ± 0,01 yumurta/birey olarak tespit edilmiş olup, söz konusu değerler kontrol grubundan istatistiksel olarak önemli derecede farlı bulunmuştur (P<0.05). Emülsiyon ilavesinin rotiferin popülayon artışı, büyüme hızı ve fekondite oranını olumlu yönde etkilediği belirlenmiştir.

Anahtar Kelimeler

Rotifer, Brachionus plicatilis, popülasyon artışı, emülsiyon

Use of Different Enrichment Emulsions in Rotifer (Brachionus plicatilis) Batch Culture

Öz

In this study, the effect of using 2 formulated emulsions (Emulsion-I and Emulsion-II) and 3 different commercial products (Olio w-3, Red Pepper, and (n-3) Top Rich) on population growth in rotifer Brachionus plicatilis culture was investigated. Experimental groups created for rotifers, respectively; DHA-Gold (Control), DHA-Gold + Emulsion-I (1st Group), DHA-Gold + Emulsion-II (2nd Group), DHA-Gold + Olio w-3 (3rd Group), DHA-Gold + Red Pepper (4th Group), DHA-Gold + (n-3) Top Rich (5th Group). To determine the population growth of rotifers according to different feeding regimes, bulk culture in 1 L culture volume was carried out with an initial density of 100 individuals/ml for 7 days and individual counts were made daily. At the end of the experiment, the highest individual number, growth rate, and fecundity ratio in the rotifers were determined as 1250 ± 11.54 individuals/ml, 0.37 ± 0.00 divisions/day, and 0.38 ± 0.01 eggs/individual in the group enriched with DHA-Gold + Emulsion-II, respectively, and these values were found to be statistically significantly different from the control group (P<0.05). It was determined that the addition of emulsion positively affected the population increase, growth rate, and fecundity rate of rotifer.

Anahtar Kelimeler

Rotifer, Brachionus plicatilis, population growth, emulsion

Kaynakça

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