Farklı Zenginleştirici Emülsiyonların Rotifer (Brachionus plicatilis) Yığın Kültüründe Kullanımı

Yıl 2022, Cilt: 18 Sayı: 1, 49 - 59, 01.03.2022

Hasan Batuhan Emre Özdoğan , Sevgi Savaş

https://doi.org/10.22392/actaquatr.950155

Cited By: 1

Öz

Bu çalışmada, rotifer Brachionus plicatilis kültüründe 2 formüle emülsiyon (Emülsiyon-I ve Emülsiyon-II) ve 3 farklı ticari ürün (Olio w-3, Red Pepper ve (n-3) Top Rich) kullanımının popülasyon artışı üzerine etkisi araştırılmıştır. Rotiferler için oluşturulan deneme grupları sırasıyla; DHA-Gold (Kontrol), DHA-Gold + Emülsiyon-I (1. Grup), DHA-Gold + Emülsiyon-II (2. Grup), DHA-Gold + Olio w-3 (3. Grup), DHA-Gold + Red Pepper (4. Grup), DHA-Gold + (n-3) Top Rich (5. Grup) olarak belirlenmiştir. Farklı besleme rejimine göre rotiferlerin populasyon artışının belirlenmesi için 1 L kültür hacminde yığın kültür, başlangıç yoğunluğu 100 birey/ml olacak şekilde 7 gün süre ile gerçekleştirilmiş ve günlük olarak birey sayımları yapılmıştır. Deneme sonunda rotiferlerde en yüksek birey sayısı, büyüme hızı ve fekondite oranı DHA-Gold + Emülsiyon-II ile zenginleştirilen grupta sırasıyla 1250 ± 11,54 birey/ml, 0,37 ± 0,00 bölünme/gün ve 0,38 ± 0,01 yumurta/birey olarak tespit edilmiş olup, söz konusu değerler kontrol grubundan istatistiksel olarak önemli derecede farlı bulunmuştur (P<0.05). Emülsiyon ilavesinin rotiferin popülayon artışı, büyüme hızı ve fekondite oranını olumlu yönde etkilediği belirlenmiştir.

Anahtar Kelimeler

Rotifer, Brachionus plicatilis, popülasyon artışı, emülsiyon

Use of Different Enrichment Emulsions in Rotifer (Brachionus plicatilis) Batch Culture

Öz

In this study, the effect of using 2 formulated emulsions (Emulsion-I and Emulsion-II) and 3 different commercial products (Olio w-3, Red Pepper, and (n-3) Top Rich) on population growth in rotifer Brachionus plicatilis culture was investigated. Experimental groups created for rotifers, respectively; DHA-Gold (Control), DHA-Gold + Emulsion-I (1st Group), DHA-Gold + Emulsion-II (2nd Group), DHA-Gold + Olio w-3 (3rd Group), DHA-Gold + Red Pepper (4th Group), DHA-Gold + (n-3) Top Rich (5th Group). To determine the population growth of rotifers according to different feeding regimes, bulk culture in 1 L culture volume was carried out with an initial density of 100 individuals/ml for 7 days and individual counts were made daily. At the end of the experiment, the highest individual number, growth rate, and fecundity ratio in the rotifers were determined as 1250 ± 11.54 individuals/ml, 0.37 ± 0.00 divisions/day, and 0.38 ± 0.01 eggs/individual in the group enriched with DHA-Gold + Emulsion-II, respectively, and these values were found to be statistically significantly different from the control group (P<0.05). It was determined that the addition of emulsion positively affected the population increase, growth rate, and fecundity rate of rotifer.

Anahtar Kelimeler

Rotifer, Brachionus plicatilis, population growth, emulsion

Kaynakça

• Anonim (2020a). DSM Birght Science. İnternet Sitesi: https://www.dsm.com/markets/anh/en_US/products/products-solutions/products-solutions dhagold.html (Son erişim tarihi: 15.06.2020).
• Anonim (2020b). Bernaqua. İnternet Sitesi: https://www.bernaqua.com (Son erişim tarihi: 15.06.2020).
• Anonim (2020c). Rich Nutritionally Rich Hatchery Diets. İnternet Sitesi: http://rich.gr (Son erişim tarihi: 15.06.2020).
• Araujo, F. G., Rosa, P. V. (2016). Docosahexaenoic acid (C22:6n-3) alters cortisol response after air exposure in Prochilodus lineatus (Valenciennes) larvae fed on enriched artemia. Aquaculture Nutrition, 23, 1216-1224. https://doi.org/10.1111/anu.12490
• Campoverde, C., & Estevez, A. (2017). The effect of live food enrichment with docosahexaenoic acid (22: 6n-3) rich emulsions on growth, survival and fatty acid composition of meagre (Argyrosomus regius) larvae. Aquaculture, 478, 16-24. https://doi.org/10.1016/j.aquaculture.2017.05.012
• Conceição, L. E., Yúfera, M., Makridis, P., Morais, S., & Dinis, M. T. (2010). Live feeds for early stages of fish rearing. Aquaculture research, 41 (5), 613-640. https://doi.org/10.1111/j.1365-2109.2009.02242.x
• Cruz-Cruz, I., Maldonado-García, M., Rebollar-Prudente, R., Estrada-Godínez, J. A., Pacheco-Vega, J. M., & Cadena-Roa, M. (2019). Nutritional value and population growth of Brachionus plicatilis fed with endemic microalgae from North Pacific. Latin American Journal Of Aquatic Research, 47 (1), 42-51. https://doi.org/10.3856/vol47-issue1-fulltext-6
• Dhert, P., Rombaut, G., Suantika, G., & Sorgeloos, P. (2001). Advancement of rotifer culture and manipulation techniques in Europe. Aquaculture, 200(1-2), 129-146. https://doi.org/10.1016/S0044-8486(01)00697-4
• Eryalçın, K. M. (2018). Effects of different commercial feeds and enrichments on biochemical composition and fatty acid profile of rotifer (Brachionus plicatilis, Müller 1786) and Artemia franciscana. Turkish Journal of Fisheries and Aquatic Sciences, 18 (1), 81-90. https://doi.org/10.4194/1303-2712-v18_1_09
• Estevez, A., & Giménez, G. (2017). Optimization of emulsion properties and enrichment conditions used in live prey enrichment. Aquaculture Nutrition, 23 (6), 1264-1273. https://doi.org/10.1111/anu.12501
• Ferreira, M., Maseda, A., Fábregas, J., & Otero, A. (2008). Enriching rotifers with “premium” microalgae. Isochrysis aff. galbana clone T-ISO. Aquaculture, 279 (1), 126-130. https://doi.org/10.1016/j.aquaculture.2008.03.044
• Flores‐Burgos, J., Sarma, S. S. S., & Nandini, S. (2005). Effect of single species or mixed algal (Chlorella vulgaris and Scenedesmus acutus) diets on the life table demography of Brachionus calyciflorus and Brachionus patulus (Rotifera: Brachionidae). Acta hydrochimica et hydrobiologica, 33 (6), 614-621. https://doi.org/10.1002/aheh.200500602
• Fuentes-Quesada, J. P., & Lazo, J. P. (2018). The effect of lipid type on lipid digestion enzymes during larval development of the California halibut, Paralichthys californicus. Aquaculture, 488, 49-60. https://doi.org/10.1016/j.aquaculture.2018.01.018
• Giménez Papiol, G., & Estévez, A. (2019). Effects of dietary arachidonic and eicosapentaenoic acids on common dentex (Dentex dentex Linnaeus 1758) larval performance. Journal of the World Aquaculture Society, 50 (5), 908-921. https://doi.org/10.1111/jwas.12599
• Giri, S. S., Sahoo, S. K., Sahu, B. B., Sahu, A. K., Mohanty, S. N., Mukhopadhyay, P. K., & Ayyappan, S. (2002). Larval survival and growth in Wallago attu (Bloch and Schneider): Effects of light, photoperiod and feeding regimes. Aquaculture, 213 (1&4), 151–161. https://doi.org/10.1016/S0044-8486(02)00012-1
• Hagiwara, A., & Marcial, H. S. (2019). The use of non-Brachionus plicatilis species complex rotifer in larviculture. Hydrobiologia, 844(1), 163-172. https://doi.org/10.1007/s10750-018-3837-z
• Hamre, K., Srivastava, A., Rønnestad, I., Mangor‐Jensen, A., & Stoss, J. (2008). Several micronutrients in the rotifer Brachionus sp. may not fulfil the nutritional requirements of marine fish larvae. Aquaculture Nutrition, 14 (1), 51-60. https://doi.org/10.1111/j.1365-2095.2007.00504.x
• Hamre, K., Yufera, M., Ronnestad, I., Boglione, C., Conceicao, L.E.C., Izquierdo, M.S. (2013). Fish larval nutrition and feed formulation knowledge gaps and bottlenecks for advances in larval rearing. Reviews in Aquaculture. 5, 526–558. https://doi.org/10.1111/j.1753-5131.2012.01086.x
• Jafari, M., Kamarudin, M. S., Saad, C. R., Arshad, A., Oryan, S., & Guilani, M. H. T. (2011). Effects of different diets on growth, survival and body composition of Rutilus frisii kutum larvae. Journal of Fisheries and Aquatic Science, 6 (6), 662–668. https://doi.org/ 10.3923/jfas.2011.662.668
• Kolkovski, S., Lazo, J., Leclercq, D., & Izquierdo, M. (2009). Fish larvae nutrition and diet: new developments. In New Technologies in Aquaculture (pp. 315-369). Woodhead Publishing. https://doi.org/10.1533/9781845696474.3.315
• Kostopoulou, V., Miliou, H., Katis, G., & Verriopoulos, G. (2006). Changes in the population structure of the lineage ‘Nevada’belonging to the Brachionus plicatilis species complex, batch-cultured under different feeding regimes. Aquaculture International, 14 (5), 451-466. https://doi.org/10.1007/s10499-006-9048-z
• Maehre, H. K., Hamre, K., Elvevoll, E. O. (2013). Nutrient evaluation of rotifers and zooplankton: feed for marine fish larvae. Aquaculture Nutrition, 19, 301-311. https://doi.org/10.1111/j.1365-2095.2012.00960.x
• Ogello, E. O., Kim, H. J., Suga, K., & Hagiwara, A. (2016). Lifetable demography and population growth of the rotifer Brachionus angularis in Kenya: influence of temperature and food density. African Journal of Aquatic Science, 41 (3), 329-336. https://doi.org/10.2989/16085914.2016.1186590
• Okunsebor, S.A. and Ayuma, V. (2011). Growths, survival rate and condition factor of Heteroclaris hatchinling fed cultured Moina micra, Shell free Artemia and combination of both as starter feed. Livestock Research for Rural Development. 23 (3), 519-525.
• Pan, L., Xi, Y. L., Cao, H. Y., Peng, B., & Wang, J. X. (2014). Combined effects of temperature and prey (Brachionus angularis) density on life-table demography and population growth of Asplanchna brightwelli (Rotifera). In Annales de Limnologie-International Journal of Limnology (Vol. 50, No. 4, pp. 261-268). EDP Sciences. https://doi.org/10.1051/limn/2014021
• Peña-Aguado, F., Nandini, S., & Sarma, S. S. S. (2005). Differences in population growth of rotifers and cladocerans raised on algal diets supplemented with yeast. Limnologica, 35 (4), 298-303. https://doi.org/10.1016/j.limno.2005.08.002
• Penglase, S., Hamre, K., Sweetman, J. W., Nordgreen, A. (2011). A new method to increase and maintain the concentration of selenium in rotifers (Brachionus spp.). Aquaculture, 315, 144-153. https://doi.org/10.1016/j.aquaculture.2010.09.007
• Qi, Z., Dierckens, K., Defoirdt, T., Sorgeloos, P., Boon, N., Bao, Z., & Bossier, P. (2009). Effects of feeding regime and probionts on the diverting microbial communities in rotifer Brachionus culture. Aquaculture International, 17 (4), 303-315. https://doi.org/10.1007/s10499-008-9202-x
• Rasdi, N. W., Ikhwannuddin, M., Azani, N., Ramlee, A., Yuslan, A., Suhaimi, H., ... & Arshad, A. (2020). The effect of different feeds on the growth, survival and reproduction of rotifer, Brachionus plicatilis. Journal of Envıronmental Bıology, 41(5), 1275-1280. https://doi.org/10.22438/jeb/41/5(SI)/MS_20
• Rebolledo, U. A., Nandini, S., Sarma, S. S. S., Reyes, J. C. R., & de Oca, G. A. R. M. (2018). Demographic and competition studies on Brachionus ibericus and Proales similis in relation to salinity and algal (Nannochloropsis oculata) density. Aquaculture International, 26 (2), 629-644. https://doi.org/10.1007/s10499-017-0233-z
• Rehberg-Haas, S., Meyer, S., Lippemeier, S., Schulz, C. (2015). A comparasion among different Pavlova sp. products for cultivation of Brachionus plicatilis. Aquaculture, 435, 424-430. https://doi.org/10.1016/j.aquaculture.2014.10.029
• Román-Padilla, J., Rodríguez-Rúa, A., Ponce, M., Manchado, M., & Hachero-Cruzado, I. (2017). Effects of dietary lipid profile on larval performance and lipid management in Senegalese sole. Aquaculture, 468, 80-93. https://doi.org/10.1016/j.aquaculture.2016.10.005
• Sahandi, J., & Jafaryan, H. (2011). Rotifer (Brachionus plicatilis) culture in batch system with suspension of algae (Nannochloropsis oculata) and bakery yeast (Saccharomyces cerevisiae). Aquaculture, Aquarium, Conservation & Legislation, 4(4), 526-529.
• Sandeep, K. P., Vasagam, K. K., & Dayal, J. S. (2015). Live Feeds and its Role in Health Management in the Larviculture of Brackishwater Finfish and Shellfishes. Dr. KK Vijayan, 121.
• Sargent, J. R., McEvoy, L. A., & Bell, J. G. (1997). Requirements, presentation and sources of polyunsaturated fatty acids in marine fish larval feeds. Aquaculture, 155 (1-4), 117-127. https://doi.org/10.1016/S0044-8486(97)00122-1
• Sargent, J., McEvoy, L., Estevez, A., Bell, G., Bell, M., Henderson, J., & Tocher, D. (1999). Lipid nutrition of marine fish during early development: current status and future directions. Aquaculture, 179 (1), 217-229. https://doi.org/10.1016/S0044-8486(99)00191-X
• Saidi, H., Morales‐Medina, R., Abrehouch, A., Fahd, S., Guadix Escobar, E. M., & Pérez‐Gálvez, R. (2018). Effect of the supplementation of live preys enriched in cod liver oil on the survival rate, growth and fatty acid profile of meagre (Argyrosomus regius) larvae. Aquaculture Research, 49 (3), 1133-1141. https://doi.org/10.1111/are.13563
• Varghese, M., & Krishnan, L. (2010). Reproductive potential of the rotifer, Brachionus rotundiformis Tschugunoff in relation to salinity, feed type and feed concentration. Indian Journal of Fisheries, 57 (1), 31-37.
• Villalta, M., Estévez, A., & Bransden, M. P. (2005). Arachidonic acid enriched live prey induces albinism in Senegal sole (Solea senegalensis) larvae. Aquaculture, 245 (1-4), 193-209. https://doi.org/10.1016/j.aquaculture.2004.11.035
• Waqalevu, V., Honda, A., Dossou, S., Khoa, T. N. D., Matsui, H., Mzengereza, K., ... & Kotani, T. (2019). Effect of oil enrichment on Brachionus plicatilis rotifer and first feeding red sea bream (Pagrus major) and Japanese flounder (Paralichthys olivaceus). Aquaculture, 510, 73-83. https://doi.org/10.1016/j.aquaculture.2019.05.039
• Yin, X. W., & Zhao, W. (2008). Studies on life history characteristics of Brachionus plicatilis OF Müller (Rotifera) in relation to temperature, salinity and food algae. Aquatic Ecology, 42(1), 165-176. https://doi.org/10.1007/s10452-007-9092-4
• Yoshimatsu, T., & Hossain, M. A. (2014). Recent advances in the high-density rotifer culture in Japan. Aquaculture international, 22 (5), 1587-1603. https://doi.org/10.1007/s10499-014-9767-5
• Zhang, D.M., Yoshimatsu, T., Furuse, M., 2005. Effect of L-carnitine enrichment on the population growth, egg ratio and body size of marine rotifer, Brachionus rotundiformis. Aquaculture, 248, 51-57. https://doi.org/10.1016/j.aquaculture.2005.04.019