Oksipösedanin kaynaklı antikanser aktivitenin in siliko ve in vitro değerlendirilmesi: Mitotoksisite?

Yıl 2023, , 153 - 161, 31.12.2023

Ali Ergüç , Hayati Okur , Fuat Karakuş , Gökay Albayrak , Ege Arzuk , Şüra Baykan

https://doi.org/10.30569/adiyamansaglik.1325975

Öz

Amaç: Çalışmanın amacı, farklı ortamlarda Oksipösedanin (OKS) aracılı antikanser aktivitedeki değişiklikleri değerlendirmektir. İkinci amaç, OKS’inin elektron transfer zincirine (ETZ) karşı afinitesini öngörmektir.
Gereç ve Yöntem: MTT ve LDH sızma deneyleri OKS ile gerçekleştirilmiştir. Ayrıca, OKS’inin ETZ komplekslerine karşı afinitesini öngörmek için moleküler kenetlenme çalışmaları uygulanmıştır.
Bulgular: Glukoz içeren ortamda 250 µM OKS canlılığı azaltmıştır. Galaktoz içeren ortamda ≥50 µM OKS hücre canlılığını azalmıştır. Galaktoz içeren ortamda ≥50 µM OKS membran parçalanmasını artırmıştır. Moleküler kenetlenme çalışmaları, OKS'inin Kompleks I ve IV'ün inhibisyon bölgelerine bağlanma kapasitesine sahip olabileceğini göstermektedir.
Sonuç: Galaktoz içeren ortam, OKS aracılı sitotoksisiteyi artırmıştır. Ön sonuçlar, antikanser aktivitede mitotoksisitenin yer alabileceğini göstermektedir. Ayrıca OKS, Kompleks I ve IV'ün seçici inhibisyonu nedeni ile ETZ disfonksiyonuna neden olabilmektedir.

Anahtar Kelimeler

Oksipösedanin, Mitotoksisite, Antikanser aktivite, İn siliko

Etik Beyan

There was no data obtained from animal or human experiments for this article.

Destekleyen Kurum

Çalışma, herhangi bir kurum tarafından desteklenmemiştir.

Proje Numarası

Çalışma, herhangi bir proje tarafından desteklenmemiştir.

Teşekkür

All of the authors gratefully thank Prof. Ayşe NALBANTSOY (Bioengineering Department, Ege University) for the laboratory facilities.

In silico and in vitro evaluation of oxypeucedanin-induced anticancer activity: Mitotoxicity?

Öz

Aim: This study aims to evaluate the alterations in Oxypeucedanin (OXY)-mediated anticancer activity in different media. Second aim is to predict the affinity of OXY to electron transfer chain (ETC) complexes.
Materials and Methods: MTT and LDH leakage assays were performed with OXY. Molecular docking studies were also conducted to predict the affinity of OXY to ETC complexes.
Results: 250 µM OXY reduced viability in glucose media. ≥50 µM OXY decreased viability in galactose media. ≥50 µM OXY increased membrane disruption in galactose media. Molecular docking studies also showed that OXY might possess the capacity to bind to the inhibition sites of Complex I and IV.
Conclusion: Galactose-conditioned media exacerbated the OXY-mediated cytotoxicity. Preliminary results suggested that mitotoxicity might take part in anticancer activity. Furthermore, OXY might cause ETC dysfunctions due to selective inhibition of Complex I and IV.

Anahtar Kelimeler

Oxypeucedanin, Mitotoxicity, Anticancer activity, In silico

Etik Beyan

There was no data obtained from animal or human experiments for this article.

Destekleyen Kurum

All of the authors declared that this article has received no financial support.

Proje Numarası

Çalışma, herhangi bir proje tarafından desteklenmemiştir.

Teşekkür

All of the authors gratefully thank Prof. Ayşe NALBANTSOY (Bioengineering Department, Ege University) for the laboratory facilities.

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