Evaluation of lung toxicity in rats kept in coal mine ambience by in vivo respiration records: An Experimental Study

Yıl 2019, , 1500 - 1510, 15.08.2019

Saliha Ayşenur Çam Seyfullah Oktay Arslan

https://doi.org/10.30569/adiyamansaglik.596944

Öz

OBJECTIVE: Mine
workers exposed to dusts or toxic gasses in occupational atmospheric
conditions. Pneumoconiosis and other lung diseases are charecterized by the
pathogenesis of coal dust-caused pulmonary toxicity associated with reactive
oxygen species (ROS).

 
MATERIALS AND METHODS:
This study was conducted to
investigate the respiration failures and fibrosis in rats after being exposed
to coal dust and gases in mine atmosphere. Another aim was to study the therapeutic
effect of erdosteine as antioxidant therapy. Rats were exposed to mine ambience
for four week, and then they were breathed in the clean air for four week. The
respiratory functions of rats were recorded once a week for eight week, as in
vivo. The fibrosis of lung tissue levels, the oxidant/antioxidant status, and
cytokines of inflammation in bronchoalveolar lavage fluids (BALFs) were
evaluated, at the end of the processes.

RESULTS: We observed to be the beginning of respiratory
abnormalities in animals exposed to coal dust in second week. The end of fourth
week, there were the increase of respiratory frequency and along with the
decrease of respiratory depth. The respiratory failures were not improved in
clean ambience, moreover apnea were appearance in the end of six week (the
second week of clean air). Deaths were 28% in animals. Erdosteine administer to
rats could not fully abolished to the pulmonary toxicity, however could able to
hold to toxicity, and also there were not dies in rats administered to
erdosteine. Coal dust exposure was resulted in fibrosis with higher hydroxyproline
(HP) levels, cytokine inflammation with higher interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α)
levels, and lipid peroxidation with an increased malondialdehyde (MDA) levels, according
to the healthy. A dramatically run out of endogen antidote sulfide pools (GSH),
and an increased MPO activity were dedected in the mine dusts and gasses
exposure group, according to the healthy animals. High biochemical index of
toxicity were partly balanced by erdosteine.

CONCLUSIONS: Our experimental findings support the hypothesis
that ROS is induced coal workers' pneumoconiosis. Re-oxygenation cannot be getting
it together to reverse the pulmonary toxicity. On the top of it, it can make
its pathogenesis further exaggerating and even worse. On this account we heartily
speculate that re-oxygenation should be by steps in mine workers. In addition
the antioxidant therapy may be partly a choice be able to tolerate the coal dust-induced
lung toxicity of mine workers.

Anahtar Kelimeler

Respiratory functions, antioxidant therapy, , re-oxygenation, oxidative stress, coal mine ambience

Kömür madeni ortamında tutulan sıçanlarda akciğer toksisitesinin in vivo solunum kayıtları ile değerlendirilmesi: Deneysel Bir Çalışma

Öz

Amaç: Maden işçileri çalışma ortamı
koşullarında tozlara veya zehirli gazlara maruz kalmaktadır. Pnömokonyoz ve
diğer akciğer hastalıkları, kömür tozu kaynaklı pulmoner toksisitenin reaktif
oksijen türleriyle (ROS) ilişkili patogenezi ile karakterizedir.

 Yöntem:
Bu çalışma, maden atmosferinde kömür tozu ve gazlarına maruz kaldıktan sonra
sıçanlarda solunum yetersizliği ve akciğer fibrozunu araştırmak için yapıldı.
Diğer bir amaç, erdosteinin antioksidan tedavi olarak terapötik etkisini
araştırmaktı. Sıçanlar, dört hafta boyunca maden ortamına maruz bırakıldı ve
daha sonra dört hafta boyunca temiz havada kaldılar. Sıçanların solunum
fonksiyonları, in vivo olarak, sekiz hafta boyunca haftada bir kez kaydedildi.
İşlem sonunda, akciğer dokusu seviyelerinin fibrozisi, oksidan / antioksidan
durumu ve bronkoalveoler lavaj sıvılarında (BALF) inflamasyonun sitokinleri
değerlendirildi.

Bulgular:
İkinci haftada kömür tozuna maruz kalan hayvanlarda solunum anormalliklerinin
başladığı gözlendi. Dördüncü haftanın sonunda, solunum sıklığının artması ve
solunum derinliğinin azalması vardı. Solunum yetersizlikleri temiz ortamda
iyileşmedi, ayrıca altı haftanın sonunda (temiz havanın ikinci haftasında) apne
ortaya çıktı. Hayvanlarda ölümler % 28 idi. Ratlara uygulanan Erdostein,
akciğer toksisitesini tamamen ortadan kaldıramadı, ancak toksisitedeki şiddeti
azalttı ve ayrıca erdostein uygulanan sıçanlarda ölen yoktu. Kömür tozuna maruz
kalma, yüksek hidroksiprolin (HP) seviyelerine sahip fibrozis, yüksek
interlökin-6 (IL-6) ve tümör nekroz faktörü-alfa (TNF-α) seviyelerinde sitokin
iltihabı ve artmış malondialdehit (MDA) ile lipit peroksidasyonuyla sonuçlandı.
Sağlıklı hayvanlara göre, endojen sülfit havuzları (GSH) dramatik bir şekilde
tükendi ve artmış bir MPO aktivitesi tespit edildi. Yüksek biyokimyasal
toksisite indeksi kısmen erdostein ile dengelenmiştir.

Sonuç: Deneysel bulgularımız, ROS'un
kömür işçilerinin pnömokonyozu oluşturduğu hipotezini desteklemektedir. Akciğer
toksisitesini tersine çevirmek için yeniden oksijenasyon yarar sağlamaz.
Üstelik, patogenezini daha da kötüleştiriyor. Bu nedenle, yeniden
oksijenasyonun maden işçilerinde adım adım olması gerektiğini kuvvetle
düşünüyoruz. Ek olarak, antioksidan tedavi, kısmen, maden işçilerinin kömür
tozu kaynaklı akciğer toksisitesini tolere edebilmesi için bir seçenek olabilir.

Anahtar Kelimeler

Solunum fonksiyonları, antioksidan tedavi, re-oksijenasyon, oksidatif stres, kömür madeni ambiyansı

Kaynakça

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