In vivo antianemic study of two species of Murva on Phenylhydrazine induced anaemia in rats

Yıl 2020, Cilt: 6 Sayı: 2, 243 - 247, 30.08.2020

Raju Asirvatham Aparna Ann Mathew Dawn V Tomy

https://doi.org/10.30569/adiyamansaglik.694909

Öz

Aim: The present study was carried out to evaluate the antianemic effects of methanol extracts of Marsdenia tenacissima and Sansevieria roxburghiana on Phenylhydrazine (PHZ) induced anaemia in rats.
Materials and Methods: PHZ (40mg/kg, i.p) injection was administered to all rats on Day 0 and Day 1 day except the group I. Group II was disease control, group III to IV treated with 100, 200 mg/Kg of methanol extract of M. tenacissima whereas group V & VI received 50, 100 mg/kg of methanol extract of S. roxburghiana. Group VII received the higher doses of both extracts continuously from the day 3 to 28. RBC, Haematocrit and Haemoglobin were analysed on day 0,2,7,14,21 and 28.
Results: Continuous 28 days oral administration extracts raised the RBC, haemoglobin, and Haematocrit periodically in rats which were treated with PHZ.
Conclusion: These plants possess antianemic activity and can be used for rapid recovery and management of anaemia.

Anahtar Kelimeler

Murva, Marsdenia tenacissima, Sansevieria roxburghiana, Phenylhydrazine, Antianemic activity

Sıçanlarda fenilhidrazin ile indüklenmiş anemi üzerine iki Murva türünün in vivo antianemik etkileri

Öz

Amaç: Bu çalışma, farelerde Marsdenia tenacissima ve Sansevieria roxburghiana'nın metanol ekstraktlarının fenilhidrazin (PHZ) ile indüklenen anemi üzerindeki antianemik etkilerini değerlendirmek için yapıldı.
Gereç ve Yöntem: Grup I hariç 0. ve 1. gün tüm sıçanlara PHZ (40 mg/kg, i.p) enjeksiyonu uygulandı. Grup II hastalık kontrolüdür, grup III ila IV, M. tenacissima'nın 100, 200 mg/kg metanol ekstraktı ile tedavi edilirken, grup V & VI 50, 100 mg/kg S. roxburghiana metanol ekstraktı almıştır. Grup VII, her iki ekstrakttan en yüksek dozları 3. ila 28. günler boyunca sürekli olarak aldı. Eritrosit, hematokrit ve hemoglobin 0, 2, 7, 14, 21 ve 28. günlerde analiz edildi.
Bulgular: PHZ ile tedavi edilen sıçanlarda periyodik olarak 28 günlük oral metanol ekstraktlarının alınması eritrosit, hematokrit ve hemoglobini yükseltti.
Sonuç: Bu bitkiler antianemik aktiviteye sahiptir ve aneminin hızlı iyileşmesi ve tedavisi için kullanılabilirler.

Anahtar Kelimeler

Murva, Marsdenia tenacissima, Sansevieria roxburghian, Fenilhidrazin, Antianemik aktivite

Kaynakça

• 1. Tettamanti M, Lucca U, Gandini F, Recchia A, Mosconi P, Apolone G, Nobili A, Tallone M V, Detoma P, Giacomin A, Clerico M, Tempia P, Savoia L, Fasolo G, Ponchio L, Della Porta MG, Riva E. Prevalence, incidence and types of mild anaemia in the elderly: the "Health and Anaemia" population-based study. Haematologica. 2010; 95(11): 1849-56.
• 2. Vijay G, Sankaran, Mitchell J Weiss. Anaemia: Progress in molecular mechanisms and therapy. Nat Med. 2015; 21(3): 221–230.
• 3. Pereira C A, Roscani M G, Zanati S G, Matsubara B B. Anaemia, heart failure and evidence-based clinical management. Arq Bras Cardiol. 2013; 101(1): 87-92.
• 4. Madeddu C, Gramignano G, Astara G, Demontis R, Sanna E, Atzeni V, Macciò A. Pathogenesis and Treatment Options of Cancer Related Anaemia: Perspective for a Targeted Mechanism-Based Approach. Front Physiol. 2018; 20(9): 1294.
• 5. Nikita H, Michael W R, Charina G. The Cardiomyopathy of Iron Deficiency. Tex Heart Inst J. 2006; 33(3): 340–344.
• 6. Jorge E T, Carlos R, Gabriel Cao, Jorge Fernando G, Felix F, Lee Mizzen, Fernando P D. Ferric carboxymaltose-mediated attenuation of Doxorubicin-induced cardiotoxicity in an iron deficiency rat model. Chemother Res Pract. 2014; 2014: 570241.
• 7. Huang MY, Zhang LL, Ding J, Lu J J. Anticancer drug discovery from Chinese medicinal herbs. Chinese medicine. 2018; 13: 35.
• 8. Varsha T, Anita S, Abhishek T. Phytopharmacological Overview on Controversial Drug: Murva. Traditional and Folk Herbal Medicine: Recent Researches. 2018; 2: 475 - 526.
• 9. Hatapakki B C, Hukkeri V. Antipyretic activity of Root of Marsdenia tenacissima in Rats. Journal of Natural Remedies. 2011; 11(2): 98-102.
• 10. Noorunissa B. Botanical Sources of Murva. ENVIS newsletter on medicinal plants. 2014; 7(1-4); 14-15.
• 11. Arabinda N, Subrata De. Phytopharmacognostic investigation of Marsdenia tenacissima (roxb) moon. World Journal of Pharmaceutical Research. 2014; 3(9); 891-904.
• 12. Manikandaselvi S, David raj C, Aravind S, Ravikumar R, Thinagarbabu R, Nandhini S. Anti-anemic activity of sprouts of Vigna radiata in male albino rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2015; 7(11): 263-267.
• 13. Gummalla P, Sravani K, Vasanthi C H, Siva Sankar Reddy K, Navyaja K, Priya V K. Evaluation of anti-anaemic and anti-allergic activity of aqueous and methanolic extracts of Ziziphus jujuba fruits in rodents. International journal of experimental pharmacology. 2015; 5(2): 91-95.
• 14. Subramani P, Gan Siaw Thing, Sokkalingam A D. Polyherbal formulation: Concept of Ayurveda. Pharmacogn Rev. 2014; 8(16): 73–80.
• 15. Droucoula G C, Kouakou S L, Miezan Bile Aka Patrice, Tchimon Danho Perrine, Yapi Houphouët Felix. Antianemic properties of Trema guineensis aqueous and ethanolic extracts. European Journal of Biotechnology and Bioscience, 2017; 5(2): 20-24.ogy and Bioscience, 2017; 5(2): 20-24.nce, 2017; 5(2): 20-24.